Anti-BCOR 抗体 (ab245423)

False colour image of Western blot: Anti-BCOR antibody staining at 1/1000 dilution, shown in black; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab245423 was shown to bind specifically to BCOR. A band was observed at 192 kDa in wild-type HeLa cell lysates with no signal observed at this size in BCOR CRISPR-Cas9 edited cell line ab265125 (CRISPR-Cas9 edited cell lysate ab258327). The band observed in the CRISPR-Cas9 edited lysate lane below 192 kDa is likely to represent a truncated form of BCOR. This has not been investigated further and the functional properties of the gene product have not been determined. To generate this image, wild-type and BCOR CRISPR-Cas9 edited HeLa cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5 % BSA in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times before development with Optiblot (ECL reagent ab133456) and imaged with 20 minutes exposure time. Secondary antibodies used were HRP conjugated Goat anti-Rabbit (H+L) and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

BCOR was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab245423 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab245423 at 1 µg/ml.

Lane 1: ab245423 IP in HeLa whole cell lysate.

Lane 2: Control IgG IP in HeLa whole cell lysate.

Detection: Chemiluminescence with an exposure time of 3 seconds.