Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
リコンビナントRabMAb

Anti-BCMA 抗体 [EPRBOB-R1-F1-24] (ab245940)

Submit a review Submit a question
Immunocytochemistry/ Immunofluorescence - Anti-BCMA antibody [EPRBOB-R1-F1-24] (ab245940)
  • Western blot - Anti-BCMA antibody [EPRBOB-R1-F1-24] (ab245940)
  • Immunoprecipitation - Anti-BCMA antibody [EPRBOB-R1-F1-24] (ab245940)
  • Flow Cytometry (Intracellular) - Anti-BCMA antibody [EPRBOB-R1-F1-24] (ab245940)
  • Anti-BCMA antibody [EPRBOB-R1-F1-24] (ab245940)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPRBOB-R1-F1-24] to BCMA
  • Suitable for: WB, ICC/IF, IP, Flow Cyt (Intra)
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-BCMA antibody [EPRBOB-R1-F1-24]
    BCMA 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPRBOB-R1-F1-24] to BCMA

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, ICC/IF, IP, Flow Cyt (Intra)more details
    適用なし: IHC-P

  • 種交差性

    交差種: Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • ICC/IF: U266B1 cells. WB: U266B1 whole cell lysate. IP: U266B1 whole cell lysate. Flow Cyt (intra): U266B1 cells.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab245940の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/1000. Predicted molecular weight: 20 kDa.
ICC/IF
1/100.
IP
1/30.
Flow Cyt (Intra)
1/60.
特記事項
WB
1/1000. Predicted molecular weight: 20 kDa.
ICC/IF
1/100.
IP
1/30.
Flow Cyt (Intra)
1/60.
追加情報
Is unsuitable for IHC-P.

ターゲット情報

  • 機能

    Receptor for TNFSF13B/BLyS/BAFF and TNFSF13/APRIL. Promotes B-cell survival and plays a role in the regulation of humoral immunity. Activates NF-kappa-B and JNK.

  • 組織特異性

    Expressed in mature B-cells, but not in T-cells or monocytes.

  • 関連疾患

    Note=A chromosomal aberration involving TNFRSF17 is found in a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(4;16)(q26;p13) with IL2.

  • 配列類似性

    Contains 1 TNFR-Cys repeat.

  • 細胞内局在

    Cell membrane. Endomembrane system. Perinuclear Golgi-like structures.
  • Information by UniProt

  • 参照データベース

    • 別名

      • B cell maturation antigen antibody
      • B cell maturation factor antibody
      • B cell maturation protein antibody
      • B-cell maturation protein antibody
      • BCM antibody
      • BCMA antibody
      • CD269 antibody
      • CD269 antigen antibody
      • TNFRSF17 antibody
      • TNR17_HUMAN antibody
      • Tumor necrosis factor receptor superfamily member 17 antibody
      see all

    画像

    • Immunocytochemistry/ Immunofluorescence - Anti-BCMA antibody [EPRBOB-R1-F1-24] (ab245940)
      Immunocytochemistry/ Immunofluorescence - Anti-BCMA antibody [EPRBOB-R1-F1-24] (ab245940)

      Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized U266B1 (human multiple myeloma B lymphocyte) cells labeling BCMA with ab245940 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining in U266B1 cell line. Negative control cells: Jurkat (PMID: 11104810. The nuclear counterstain is DAPI (blue).
      Counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at a 1/200 dilution (red). The negative control is the secondary antibody only.

    • Western blot - Anti-BCMA antibody [EPRBOB-R1-F1-24] (ab245940)
      Western blot - Anti-BCMA antibody [EPRBOB-R1-F1-24] (ab245940)
      All lanes : Anti-BCMA antibody [EPRBOB-R1-F1-24] (ab245940) at 1/300 dilution

      Lane 1 : Jurkat (human T cell leukemia T lymphocyte) whole cell lysate
      Lane 2 : U266B1 (human multiple myeloma B lymphocyte) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 20 kDa
      Observed band size: 19,23-30 kDa why is the actual band size different from the predicted?


      Exposure time: 3 minutes


      The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 23776238).

      Negative control: Jurkat (PMID: 11104810).

      Blocking/Dilution buffer: 5% NFDM/TBST.

    • Immunoprecipitation - Anti-BCMA antibody [EPRBOB-R1-F1-24] (ab245940)
      Immunoprecipitation - Anti-BCMA antibody [EPRBOB-R1-F1-24] (ab245940)

      BCMA was immunoprecipitated from 0.35 mg U266B1 (human multiple myeloma B lymphocyte) whole cell lysate with ab245940 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab245940 at 1/300 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used at 1/5000 dilution.

      Lane 1: U266B1 whole cell lysate 10 µg (Input).
      Lane 2: ab245940 IP in U266B1 whole cell lysate.
      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab245940 in U266B1 whole cell lysate.

      Blocking/Dilution buffer: 5% NFDM/TBST.
      Exposure time: 30 seconds.

    • Flow Cytometry (Intracellular) - Anti-BCMA antibody [EPRBOB-R1-F1-24] (ab245940)
      Flow Cytometry (Intracellular) - Anti-BCMA antibody [EPRBOB-R1-F1-24] (ab245940)

      Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized U266B1 (human multiple myeloma B lymphocyte) cell line labeling BCMA with ab245940 at 1/60 (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody. 

       

       

    • Anti-BCMA antibody [EPRBOB-R1-F1-24] (ab245940)
      Anti-BCMA antibody [EPRBOB-R1-F1-24] (ab245940)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab245940 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab245940 は論文での使用が確認できていません。

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    There are currently no Customer reviews or Questions for ab245940.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com