Anti-Bcl10 抗体 [EPR8587] (ab150380)
![Western blot - Anti-Bcl10 antibody [EPR8587] (ab150380)](https://www.abcam.co.jp/ps/products/150/ab150380/Images/ab150380-384458-anti-bcl10-antibody-epr8587-western-blot-hela-ramos-a549.jpg "Western blot - Anti-Bcl10 antibody [EPR8587] (ab150380)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8587] to Bcl10
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Bcl10 antibody [EPR8587]
Bcl10 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR8587] to Bcl10 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details
適用なし: Flow Cyt -
種交差性
交差種: Human -
免疫原
Recombinant fragment corresponding to Human Bcl10 aa 1-250.
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ポジティブ･コントロール
- WB: Recombinant Human Bcl10 protein (ab124575); Ramos, HeLa, HuT-78 and Raji cell lysates. IHC-P: Human MALT and tonsil tissues.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR8587 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab150380の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 26 kDa.
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| IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 特記事項 |
|---|
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WB
1/1000 - 1/10000. Predicted molecular weight: 26 kDa. |
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IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Promotes apoptosis, pro-caspase-9 maturation and activation of NF-kappa-B via NIK and IKK. May be an adapter protein between upstream TNFR1-TRADD-RIP complex and the downstream NIK-IKK-IKAP complex. Is a substrate for MALT1. -
組織特異性
Ubiquitous. -
関連疾患
Note=A chromosomal aberration involving BCL10 is recurrent in low-grade mucosa-associated lymphoid tissue (MALT lymphoma). Translocation t(1;14)(p22;q32). Although the BCL10/IgH translocation leaves the coding region of BCL10 intact, frequent BCL10 mutations could be attributed to the Ig somatic hypermutation mechanism resulting in nucleotide transitions.
Note=Defects in BCL10 are involved in various types of cancer. -
配列類似性
Contains 1 CARD domain. -
翻訳後修飾
Phosphorylated. Phosphorylation results in dissociation from TRAF2 and binding to BIRC2/c-IAP2. -
細胞内局在
Cytoplasm > perinuclear region. Membrane raft. Appears to have a perinuclear, compact and filamentous pattern of expression. Also found in the nucleus of several types of tumor cells. Colocalized with DPP4 in membrane rafts. - Information by UniProt
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参照データベース
- Entrez Gene: 8915 Human
- Omim: 603517 Human
- SwissProt: O95999 Human
- Unigene: 193516 Human
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別名
- AI132454 antibody
- B cell CLL/lymphoma 10 antibody
- B cell lymphoma/leukemia10 antibody
see all
画像
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Western blot - Anti-Bcl10 antibody [EPR8587] (ab150380)All lanes : Anti-Bcl10 antibody [EPR8587] (ab150380) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : BCL10 knockout HeLa cell lysate
Lane 3 : Romas cell lysate
Lane 4 : A549 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 26 kDa
Observed band size: 32 kDa why is the actual band size different from the predicted?Lanes 1-4: Merged signal (red and green). Green - ab150380 observed at 32 kDa. Red - loading control, ab7291 observed at 52 kDa.
ab150380 Anti-Bcl10 antibody [EPR8587] was shown to specifically react with Bcl10 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab261797 (knockout cell lysate ab257144) was used. Wild-type and Bcl10 knockout samples were subjected to SDS-PAGE. ab150380 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-Bcl10 antibody [EPR8587] (ab150380)](https://www.abcam.co.jp/ps/products/150/ab150380/Images/ab150380-156969-anti-bcl10-antibody-epr8587-western-blot.jpg)
Western blot - Anti-Bcl10 antibody [EPR8587] (ab150380)All lanes : Anti-Bcl10 antibody [EPR8587] (ab150380) at 1/1000 dilution
Lane 1 : Ramos cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : HuT-78 cell lysate
Lane 4 : Raji cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labeled goat anti-rabbit antibody at 1/2000 dilution
Predicted band size: 26 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl10 antibody [EPR8587] (ab150380)](https://www.abcam.co.jp/ps/products/150/ab150380/Images/ab150380-156968-anti-bcl10-antibody-epr8587-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl10 antibody [EPR8587] (ab150380)Immunohistochemical analysis of paraffin embedded Human MALT tissue labeling Bcl10 with ab150380 at a 1/250 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl10 antibody [EPR8587] (ab150380)](https://www.abcam.co.jp/ps/products/150/ab150380/Images/ab150380-156967-anti-bcl10-antibody-epr8587-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl10 antibody [EPR8587] (ab150380)Immunohistochemical analysis of paraffin embedded Human tonsil tissue labeling Bcl10 with ab150380 at a 1/250 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Anti-Bcl10 antibody [EPR8587] (ab150380)](https://www.abcam.co.jp/ps/products/150/ab150380/Images/ab150380-5-benefits-of-recombinant-antibodies.png)
Anti-Bcl10 antibody [EPR8587] (ab150380)
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab150380 は論文での使用が確認できていません。