Anti-Bcl10 抗体 [4F8E8H8] (ab36585)
Key features and details
- Mouse monoclonal [4F8E8H8] to Bcl10
- Suitable for: IHC-P, WB, Flow Cyt, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG1
製品の概要
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製品名
Anti-Bcl10 antibody [4F8E8H8]
Bcl10 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [4F8E8H8] to Bcl10 -
由来種
Mouse -
アプリケーション
適用あり: IHC-P, WB, Flow Cyt, ICC/IFmore details -
種交差性
交差種: Mouse, Human -
免疫原
Ni-NTA purified recombinant human BCL-10 expressed in E. Coli strain BL21 (DE3).
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ポジティブ・コントロール
- MCF-7 cell extract. Human breast and liver carcinoma tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading...
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精製度
Protein G purified -
特記事項(精製)
Purified from tissue culture supernatant. -
ポリ/モノ
モノクローナル -
クローン名
4F8E8H8 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab36585の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
Use at an assay dependent concentration.
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WB |
1/500 - 1/2000. Detects a band of approximately 34 kDa (predicted molecular weight: 31 kDa).
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Flow Cyt |
1/200.
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ICC/IF |
1/200 - 1/1000.
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特記事項 |
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IHC-P
Use at an assay dependent concentration. |
WB
1/500 - 1/2000. Detects a band of approximately 34 kDa (predicted molecular weight: 31 kDa). |
Flow Cyt
1/200. |
ICC/IF
1/200 - 1/1000. |
ターゲット情報
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機能
Promotes apoptosis, pro-caspase-9 maturation and activation of NF-kappa-B via NIK and IKK. May be an adapter protein between upstream TNFR1-TRADD-RIP complex and the downstream NIK-IKK-IKAP complex. Is a substrate for MALT1. -
組織特異性
Ubiquitous. -
関連疾患
Note=A chromosomal aberration involving BCL10 is recurrent in low-grade mucosa-associated lymphoid tissue (MALT lymphoma). Translocation t(1;14)(p22;q32). Although the BCL10/IgH translocation leaves the coding region of BCL10 intact, frequent BCL10 mutations could be attributed to the Ig somatic hypermutation mechanism resulting in nucleotide transitions.
Note=Defects in BCL10 are involved in various types of cancer. -
配列類似性
Contains 1 CARD domain. -
翻訳後修飾
Phosphorylated. Phosphorylation results in dissociation from TRAF2 and binding to BIRC2/c-IAP2. -
細胞内局在
Cytoplasm > perinuclear region. Membrane raft. Appears to have a perinuclear, compact and filamentous pattern of expression. Also found in the nucleus of several types of tumor cells. Colocalized with DPP4 in membrane rafts. - Information by UniProt
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参照データベース
- Entrez Gene: 8915 Human
- Entrez Gene: 12042 Mouse
- Omim: 603517 Human
- SwissProt: O95999 Human
- SwissProt: Q9Z0H7 Mouse
- Unigene: 193516 Human
- Unigene: 239141 Mouse
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別名
- AI132454 antibody
- B cell CLL/lymphoma 10 antibody
- B cell lymphoma/leukemia10 antibody
see all
画像
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All lanes : Anti-Bcl10 antibody [4F8E8H8] (ab36585) at 1/500 dilution
Lane 1 : Cell lysates prepared from NIH/3T3 cells
Lane 2 : Cell lysates prepared from Hela cells
Lane 3 : Cell lysates prepared from MCF-7 cells
Lane 4 : Cell lysates prepared from Jurkat cells
Lysates/proteins at 100 µg per lane.
Secondary
All lanes : HRP-conjugated Goat polyclonal to mouse IgG1
Predicted band size: 31 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl10 antibody [4F8E8H8] (ab36585)Ab36585, at a 1/100 - 1/500 dilution, staining Bcl-10 in paraffin embedded human liver carcinoma tissue by immunohistochemistry with DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl10 antibody [4F8E8H8] (ab36585)Ab36585, at a 1/100 - 1/500 dilution, staining Bcl-10 in paraffin embedded human breast carcinoma tissue by immunohistochemistry with DAB staining.
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ab36585 at 1/1000 dilution staining Bcl10 in human Hela cells by Immunocytochemistry/ Immunofluorescence. Bcl10 show green staining in image and actin filament stained red with Alexa Fluor®555 conjugated secondary. Nuclei stained blue with DRAQ5 fluorescent DNA dye.
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Flow cytometric analysis of Hela cells using ab36585 at a 1/200 dilution (green) and negative control (purple).
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab36585 は論文での使用が確認できていません。