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リコンビナント

Anti-Bcl-XL 抗体 [2H12] (ab77571)

Submit a review Submit a question References (4)
Western blot - Anti-Bcl-XL antibody [2H12] (ab77571)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-XL antibody [2H12] (ab77571)
  • Western blot - Anti-Bcl-XL antibody [2H12] (ab77571)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-XL antibody [2H12] (ab77571)
  • Anti-Bcl-XL antibody [2H12] (ab77571)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [2H12] to Bcl-XL
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-Bcl-XL antibody [2H12]
    Bcl-XL 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [2H12] to Bcl-XL

  • 由来種

    Mouse

  • アプリケーション

    適用あり: IHC-P, WBmore details
    適用なし: Flow Cyt,ICC or IP

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • IHC-P: Human colon carcinoma tissue and kidney tissue. WB: Ramos, HCT 116, C6 and NIH/3T3 whole cell lysate.

  • 特記事項

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab77571の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
1/1000. Predicted molecular weight: 26 kDa.
特記事項
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
1/1000. Predicted molecular weight: 26 kDa.
追加情報
Is unsuitable for Flow Cyt,ICC or IP.

ターゲット情報

  • 機能

    Potent inhibitor of cell death. Inhibits activation of caspases (By similarity). Appears to regulate cell death by blocking the voltage-dependent anion channnel (VDAC) by binding to it and preventing the release of the caspase activator, CYC1, from the mitochondrial membrane.
    Isoform Bcl-X(S) promotes apoptosis.

  • 組織特異性

    Bcl-X(S) is expressed at high levels in cells that undergo a high rate of turnover, such as developing lymphocytes. In contrast, Bcl-X(L) is found in tissues containing long-lived postmitotic cells, such as adult brain.

  • 配列類似性

    Belongs to the Bcl-2 family.

  • ドメイン

    The BH4 motif is required for anti-apoptotic activity. The BH1 and BH2 motifs are required for both heterodimerization with other Bcl-2 family members and for repression of cell death.

  • 翻訳後修飾

    Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity.

  • 細胞内局在

    Mitochondrion membrane. Nucleus membrane. Mitochondrial membranes and perinuclear envelope.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • Apoptosis regulator Bcl X antibody
    • Apoptosis regulator Bcl-X antibody
    • Apoptosis regulator BclX antibody
    • B cell lymphoma 2 like antibody
    • B2CL1_HUMAN antibody
    • Bcl 2 like 1 protein antibody
    • Bcl X antibody
    • Bcl xL antibody
    • BCL XL/S antibody
    • Bcl xS antibody
    • Bcl-2-like protein 1 antibody
    • Bcl2 Like 1 antibody
    • Bcl2 related gene antibody
    • Bcl2-L-1 antibody
    • BCL2L antibody
    • Bcl2l1 antibody
    • BCLX antibody
    • BclXL antibody
    • BclXs antibody
    • DKFZp781P2092 antibody
    • PPP1R52 antibody
    • Protein phosphatase 1 regulatory subunit 52 antibody
    see all

画像

  • Western blot - Anti-Bcl-XL antibody [2H12] (ab77571)
    Western blot - Anti-Bcl-XL antibody [2H12] (ab77571)
    All lanes : Anti-Bcl-XL antibody [2H12] (ab77571) at 1/1000 dilution

    Lane 1 : Ramos (human Burkitt's lymphoma B lymphocyte), whole cell lysate
    Lane 2 : HCT 116 (human colorectal carcinoma epithelial cell), whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/5000 dilution

    Predicted band size: 26 kDa
    Observed band size: 26 kDa



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-XL antibody [2H12] (ab77571)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-XL antibody [2H12] (ab77571)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling Bcl-XL with ab77571 at 1/100 dilution followed by ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879). Cytoplasmic staining on human kidney. The section was incubated with ab77571 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Western blot - Anti-Bcl-XL antibody [2H12] (ab77571)
    Western blot - Anti-Bcl-XL antibody [2H12] (ab77571)
    All lanes : Anti-Bcl-XL antibody [2H12] (ab77571) at 1/1000 dilution

    Lane 1 : C6 (rat glial tumor glial cell), whole cell lysate
    Lane 2 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/5000 dilution

    Predicted band size: 26 kDa
    Observed band size: 26 kDa



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    This blot was developed using a higher sensitivity ECL substrate.

    Exposure time: 37 seconds.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-XL antibody [2H12] (ab77571)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-XL antibody [2H12] (ab77571)

    Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue labeling Bcl-XL with ab77571 at 1/100 dilution followed by ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879). Cytoplasmic staining on human colon carcinoma. The section was incubated with ab77571 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Anti-Bcl-XL antibody [2H12] (ab77571)
    Anti-Bcl-XL antibody [2H12] (ab77571)

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (4)

ab77571 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab77571 は 4 報の論文で使用されています。

  • Cardile A  et al. Hyperforin Elicits Cytostatic/Cytotoxic Activity in Human Melanoma Cell Lines, Inhibiting Pro-Survival NF-κB, STAT3, AP1 Transcription Factors and the Expression of Functional Proteins Involved in Mitochondrial and Cytosolic Metabolism. Int J Mol Sci 24:N/A (2023). PubMed: 36674794
  • Xu G  et al. Glucocappasalin Induces G2/M-Phase Arrest, Apoptosis, and Autophagy Pathways by Targeting CDK1 and PLK1 in Cervical Carcinoma Cells. Front Pharmacol 12:671138 (2021). PubMed: 34093198
  • Zhong B  et al. Caspase-8 Induces Lysosome-Associated Cell Death in Cancer Cells. Mol Ther 28:1078-1091 (2020). PubMed: 32053770
  • Yin P  et al. ABT-737, a Bcl-2 Selective Inhibitor, and Chloroquine Synergistically Kill Renal Cancer Cells. Oncol Res 24:65-72 (2016). PubMed: 27178823

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