Anti-BCAS3 抗体 (ab71162)
Key features and details
- Rabbit polyclonal to BCAS3
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-BCAS3 antibody
BCAS3 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to BCAS3 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IP, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rhesus monkey, Orangutan
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免疫原
A synthetic peptide corresponding to a region between residue 870 and the C terminus (residue 928) of human Breast carcinoma amplified sequence 3 (BAB15156.1)
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ポジティブ･コントロール
- Whole cell lysate from MCF7 cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab71162の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/2000 - 1/10000. Detects a band of approximately 117 kDa (predicted molecular weight: 101 kDa).
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| IP |
Use at 2-5 µg/mg of lysate.
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| IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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| 特記事項 |
|---|
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WB
1/2000 - 1/10000. Detects a band of approximately 117 kDa (predicted molecular weight: 101 kDa). |
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IP
Use at 2-5 µg/mg of lysate. |
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IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
ターゲット情報
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組織特異性
Expressed in stomach, liver, lung, kidney, prostate, testis, thyroid gland, adrenal gland, brain, heart, skeletal muscle, colon, spleen, small intestine, placenta, blood leukocyte and mammary epithelial cells. Overexpressed in most breast cancer cell lines. -
関連疾患
Note=A chromosomal aberration involving BCAS3 has been found in some breast carcinoma cell lines. Translocation t(17;20)(q23;q13) with BCAS4. -
配列類似性
Belongs to the WD repeat BCAS3 family.
Contains 3 WD repeats. -
発生段階
Fetal. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 54828 Human
- Entrez Gene: 192197 Mouse
- Omim: 607470 Human
- SwissProt: Q9H6U6 Human
- SwissProt: Q8CCN5 Mouse
- Unigene: 655028 Human
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別名
- 1500019F07Rik antibody
- 2610028P08Rik antibody
- AU021018 antibody
see all
画像
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Immunoprecipitation - Anti-BCAS3 antibody (ab71162)Immunoprecipitation/ Western Blot of Breast carcinoma amplified sequence 3.
Lane 1: ab71162 at 3µg/mg whole cell lysate. Lane 2: Control IgG.
All lanes: Whole cell lysate from MCF7 cells at 1mg for IP, 20% of IP loaded. Detection: Chemiluminescence with an exposure time of 1 second. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCAS3 antibody (ab71162)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling BCAS3 with ab71162 at 1/200 (1µg/ml). Detection: DAB. -
Western blot - Anti-BCAS3 antibody (ab71162)All lanes : Anti-BCAS3 antibody (ab71162) at 0.04 µg/ml
Lane 1 : Whole cell lysate from MCF7 cells at 50 µg
Lane 2 : Whole cell lysate from MCF7 cells at 15 µg
Lane 3 : Whole cell lysate from MCF7 cells at 5 µg
Developed using the ECL technique.
Predicted band size: 101 kDa
Observed band size: 117 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab71162 は論文での使用が確認できていません。