Anti-BCAR1 (phospho Y762) 抗体 (ab41827)
Key features and details
- Rabbit polyclonal to BCAR1 (phospho Y762)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-BCAR1 (phospho Y762) antibody
BCAR1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to BCAR1 (phospho Y762) -
由来種
Rabbit -
特異性
This antibody detects a 130 kDa protein corresponding to the molecular mass of phosphorylated BCAR1 on SDS-PAGE immunoblots of pervanadate treated human A431 cells, but not in control cells. -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to Rat BCAR1 (phospho Y762).
Database link: Q63767 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol, 0.1% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
This antibody was cross-adsorbed to phospho-tyrosine coupled to agarose and to phospho-BCAR1(Tyr-751) peptide before affinity purification using phospho-BCAR1(Tyr-762) peptide (without carrier). -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab41827の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/2000. Predicted molecular weight: 130 kDa.
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| 特記事項 |
|---|
|
WB
1/2000. Predicted molecular weight: 130 kDa. |
ターゲット情報
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機能
Docking protein which plays a central coordinating role for tyrosine kinase-based signaling related to cell adhesion. Implicated in induction of cell migration. Overexpression confers antiestrogen resistance on breast cancer cells. -
組織特異性
Widely expressed with an abundant expression in the testis. Low level of expression seen in the liver, thymus, and peripheral blood leukocytes. The protein has been detected in a B-cell line. -
配列類似性
Belongs to the CAS family.
Contains 1 SH3 domain. -
ドメイン
Contains a central domain (substrate domain) containing multiple potential SH2-binding sites and a C-terminal domain containing a divergent helix-loop-helix (HLH) motif. The SH2-binding sites putatively bind CRK, NCK and ABL1 SH2 domains. The HLH motif is absolutely required for the induction of pseudohyphal growth in yeast and mediates heterodimerization with NEDD9.
A serine-rich region promotes activation of the serum response element (SRE).
The SH3 domain is necessary for the localization of the protein to focal adhesions and interacts with one proline-rich region of PTK2/FAK11. -
翻訳後修飾
PTK2/FAK1 activation mediates phosphorylation at the YDYVHL motif; phosphorylation is most likely catalyzed by SRC family members. SRC-family kinases are recruited to the phosphorylated sites and can phosphorylate other tyrosine residues. Tyrosine phosphorylation is triggered by integrin-mediated adhesion of cells to the extracellular matrix.
Dephosphorylated by PTPN14 at Tyr-128. -
細胞内局在
Cell junction, focal adhesion. Cytoplasm. Unphosphorylated form localizes in the cytoplasm and can move to the membrane upon tyrosine phosphorylation. - Information by UniProt
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参照データベース
- Entrez Gene: 9564 Human
- Omim: 602941 Human
- SwissProt: P56945 Human
- Unigene: 479747 Human
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別名
- BCAR 1 antibody
- Bcar1 antibody
- BCAR1_HUMAN antibody
see all
画像
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Western blot - Anti-BCAR1 (phospho Y762) antibody (ab41827)Western blot analysis of A431 cells serum starved overnight (lanes 1 & 3) or treated with pervanadate (1 mM) for 30 minutes (lanes 2, 4-6). The blot was probed with anti-BCAR1 (lanes 1 & 2) or anti-BCAR1 (Tyr-762) (lanes 3-6). The latter was used in the presence of no peptide (lane 4), phospho-p130 Cas (Tyr-762) peptide (lane 5) or phospho-BCAR1 (Tyr-751) peptide (lane 6).
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab41827 は 1 報の論文で使用されています。
- Zheng Y et al. Protein-tyrosine Kinase 6 Promotes Peripheral Adhesion Complex Formation and Cell Migration by Phosphorylating p130 CRK-associated Substrate. J Biol Chem 287:148-58 (2012). ICC/IF ; Human . PubMed: 22084245