Anti-BCAR1 抗体 [M144] (ab31831)
![Western blot - Anti-BCAR1 antibody [M144] (ab31831)](https://www.abcam.co.jp/ps/products/31/ab31831/Images/ab31831-43067-anti-bcar1-antibody-m144-western-blot.jpg "Western blot - Anti-BCAR1 antibody [M144] (ab31831)")
Key features and details
- Mouse monoclonal [M144] to BCAR1
- Suitable for: IHC-P, Flow Cyt (Intra), WB
- Reacts with: Human
- Isotype: IgG1
製品の概要
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製品名
Anti-BCAR1 antibody [M144]
BCAR1 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [M144] to BCAR1 -
由来種
Mouse -
特異性
ab31831 detects a 130 kDa protein corresponding to the molecular mass of p130 Cas on SDS-PAGE immunoblots of human A431, endothelial, and Hct116 cells. -
アプリケーション
適用あり: IHC-P, Flow Cyt (Intra), WBmore details -
種交差性
交差種: Human -
免疫原
Recombinant fragment corresponding to Rat BCAR1 aa 600-850 (C terminal).
Database link: Q63767 -
エピトープ
ab31831 recognises an epitope located in teh C terminal region of BCAR1. -
ポジティブ･コントロール
- Human A431, endothelial, and Hct116 cells. IHC-P: Human testis tissue sections. Flow Cyt (intra): MCF7 cells
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol, 0.1% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
M144 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab31831の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
1/250.
|
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| Flow Cyt (Intra) |
1/100.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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| WB |
1/1000. Predicted molecular weight: 93 kDa.
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| 特記事項 |
|---|
|
IHC-P
1/250. |
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Flow Cyt (Intra)
1/100. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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WB
1/1000. Predicted molecular weight: 93 kDa. |
ターゲット情報
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機能
Docking protein which plays a central coordinating role for tyrosine kinase-based signaling related to cell adhesion. Implicated in induction of cell migration. Overexpression confers antiestrogen resistance on breast cancer cells. -
組織特異性
Widely expressed with an abundant expression in the testis. Low level of expression seen in the liver, thymus, and peripheral blood leukocytes. The protein has been detected in a B-cell line. -
配列類似性
Belongs to the CAS family.
Contains 1 SH3 domain. -
ドメイン
Contains a central domain (substrate domain) containing multiple potential SH2-binding sites and a C-terminal domain containing a divergent helix-loop-helix (HLH) motif. The SH2-binding sites putatively bind CRK, NCK and ABL1 SH2 domains. The HLH motif is absolutely required for the induction of pseudohyphal growth in yeast and mediates heterodimerization with NEDD9.
A serine-rich region promotes activation of the serum response element (SRE).
The SH3 domain is necessary for the localization of the protein to focal adhesions and interacts with one proline-rich region of PTK2/FAK11. -
翻訳後修飾
PTK2/FAK1 activation mediates phosphorylation at the YDYVHL motif; phosphorylation is most likely catalyzed by SRC family members. SRC-family kinases are recruited to the phosphorylated sites and can phosphorylate other tyrosine residues. Tyrosine phosphorylation is triggered by integrin-mediated adhesion of cells to the extracellular matrix.
Dephosphorylated by PTPN14 at Tyr-128. -
細胞内局在
Cell junction, focal adhesion. Cytoplasm. Unphosphorylated form localizes in the cytoplasm and can move to the membrane upon tyrosine phosphorylation. - Information by UniProt
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参照データベース
- Entrez Gene: 9564 Human
- Omim: 602941 Human
- SwissProt: P56945 Human
- Unigene: 479747 Human
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別名
- BCAR 1 antibody
- Bcar1 antibody
- BCAR1_HUMAN antibody
see all
画像
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Western blot - Anti-BCAR1 antibody [M144] (ab31831)Western blot analysis of human endothelial cells serum starved overnight (lanes 1, 3, & 5) or treated with pervanadate (1 mM) for 30 minutes (lanes 2, 4, & 6). The blot was probed with anti-BCAR1 (ab31831; lanes 1 & 2), anti-BCAR1 (Tyr-751) (lanes 3 & 4) or anti-BCAR1 (Tyr-762) (lanes 5 & 6).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCAR1 antibody [M144] (ab31831)](https://www.abcam.co.jp/ps/products/31/ab31831/Images/ab31831-200171-anti-bcar1-antibody-m144-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCAR1 antibody [M144] (ab31831)IHC image of BCAR1 staining in human testis formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab31831, 1/200 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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![Flow Cytometry (Intracellular) - Anti-BCAR1 antibody [M144] (ab31831)](https://www.abcam.co.jp/ps/products/31/ab31831/Images/ab31831-43066-anti-bcar1-antibody-m144-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-BCAR1 antibody [M144] (ab31831)Overlay histogram showing MCF7 cells stained with ab31831 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab31831, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab31831 は 5 報の論文で使用されています。
- Dybdal-Hargreaves NF et al. Correction: Regulation of E-cadherin localization by microtubule targeting agents: rapid promotion of cortical E-cadherin through p130CAS/Src inhibition by eribulin. Oncotarget 10:4719-4720 (2019). PubMed: 31384398
- Heumann A et al. High BCAR1 expression is associated with early PSA recurrence in ERG negative prostate cancer. BMC Cancer 18:37 (2018). IHC-P . PubMed: 29304771
- Beck TN et al. EGFR and RB1 as Dual Biomarkers in HPV-Negative Head and Neck Cancer. Mol Cancer Ther 15:2486-2497 (2016). PubMed: 27507850
- Yuan L & Liu X Platelets are associated with xenograft tumor growth and the clinical malignancy of ovarian cancer through an angiogenesis-dependent mechanism. Mol Med Rep 11:2449-58 (2015). IHC-P ; Human . PubMed: 25502723
- Miao Y et al. Expression of p130cas, E-cadherin and ß-catenin and their correlation with clinicopathological parameters in non-small cell lung cancer: p130cas over-expression predicts poor prognosis. Folia Histochem Cytobiol 50:392-7 (2012). PubMed: 23042269