Anti-BAZ2A/TIP5 抗体 [EPR25419-10] - BSA and Azide free (ab290650)
![Western blot - Anti-BAZ2A/TIP5 antibody [EPR25419-10] - BSA and Azide free (ab290650)](https://www.abcam.co.jp/ps/products/290/ab290650/Images/ab290650-484250-WB1.jpg "Western blot - Anti-BAZ2A/TIP5 antibody [EPR25419-10] - BSA and Azide free (ab290650)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR25419-10] to BAZ2A/TIP5 - BSA and Azide free
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-BAZ2A/TIP5 antibody [EPR25419-10] - BSA and Azide free
BAZ2A/TIP5 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR25419-10] to BAZ2A/TIP5 - BSA and Azide free -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details
適用なし: ChIP,Flow Cyt (Intra),ICC/IF,IHC-P or IP -
種交差性
交差種: Human
非交差種: Mouse, Rat -
免疫原
Recombinant fragment within Human BAZ2A/TIP5 aa 1-200. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q9UIF9 -
ポジティブ･コントロール
- WB: HeLa (human cervix adenocarcinoma epithelial cell) whole cell fresh lysate, 293T (human embryonic kidney epithelial cell) whole cell fresh lysate, MDA-MB-231 (human breast adenocarcinoma epithelial cell) whole cell fresh lysate, PC-3 (human prostate adenocarcinoma epithelial cell) whole cell fresh lysate.
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特記事項
ab290650 is a carrier free version of ab290639.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 7.2
Constituent: 100% PBS -
キャリア・フリー
はい -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR25419-10 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab290650の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use at an assay dependent concentration. Predicted molecular weight: 208 kDa.
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| 特記事項 |
|---|
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WB
Use at an assay dependent concentration. Predicted molecular weight: 208 kDa. |
ターゲット情報
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関連性
BAZ2A is a bromodomain adjacent to a zinc finger domain protein. It may play a role in transcriptional regulation interacting with ISWI and may serve a specific role in maintaining or altering the chromatin structure of the rDNA locus. BAz2A colocalizes with the basal RNA polymerase I transcription factor UBF in the nucleolus. -
細胞内局在
Nuclear -
参照データベース
- Entrez Gene: 11176 Human
- Omim: 605682 Human
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別名
- Bromodomain adjacent to zinc finger domain 2A antibody
- Tip5 antibody
- Transcription termination factor I interacting protein 5 antibody
see all
画像
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Western blot - Anti-BAZ2A/TIP5 antibody [EPR25419-10] - BSA and Azide free (ab290650)All lanes : Anti-BAZ2A/TIP5 antibody [EPR25419-10] (ab290639) at 1/1000 dilution
Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell fresh lysate
Lane 2 : 293T (human embryonic kidney epithelial cell) whole cell fresh lysate
Lane 3 : MDA-MB-231 (human breast adenocarcinoma epithelial cell) whole cell fresh lysate
Lane 4 : PC-3 (human prostate adenocarcinoma epithelial cell) whole cell fresh lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 208 kDa
Observed band size: 270 kDa why is the actual band size different from the predicted?This data was develop using ab290639, the same antibody clone in different buffer formulation.
Blocking and diluting buffers and concentration was 5% NFDM/TBST.
The molecular weight observed is consistent with that described in the literature (PMID: 19578370)
Lysates were prepared from fresh materials and used for Western blotting immediately to minimize protein degradation.Exposure time:
Lane 1-3: 15 seconds
Lane 4: 26 seconds -
![Anti-BAZ2A/TIP5 antibody [EPR25419-10] - BSA and Azide free (ab290650)](https://www.abcam.co.jp/ps/products/290/ab290650/Images/ab290650-484338-Benefits-of-Recombinant-antibodies.jpg)
Anti-BAZ2A/TIP5 antibody [EPR25419-10] - BSA and Azide free (ab290650)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab290650 は論文での使用が確認できていません。
