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リコンビナントRabMAb

Anti-BATF3 抗体 [EPR26558-38] (ab302568)

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Western blot - Anti-BATF3 antibody [EPR26558-38] (AB302568)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BATF3 antibody [EPR26558-38] (AB302568)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BATF3 antibody [EPR26558-38] (AB302568)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BATF3 antibody [EPR26558-38] (AB302568)
  • Immunocytochemistry/ Immunofluorescence - Anti-BATF3 antibody [EPR26558-38] (ab302568)
  • Flow Cytometry (Intracellular) - Anti-BATF3 antibody [EPR26558-38] (AB302568)
  • Flow Cytometry (Intracellular) - Anti-BATF3 antibody [EPR26558-38] (AB302568)
  • Immunoprecipitation - Anti-BATF3 antibody [EPR26558-38] (AB302568)
  • Anti-BATF3 antibody [EPR26558-38] (AB302568)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR26558-38] to BATF3
  • Suitable for: ICC/IF, IP, IHC-P, Flow Cyt (Intra), WB
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 647 Carrier Free

製品の概要

  • 製品名

    Anti-BATF3 antibody [EPR26558-38]
    BATF3 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR26558-38] to BATF3

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: ICC/IF, IP, IHC-P, Flow Cyt (Intra), WBmore details

  • 種交差性

    交差種: Human
    非交差種: Mouse, Rat

  • 免疫原

    Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Whole cell lysates: KARPAS-299 (human T cell lymphoma cell), HDLM-2 (human Hodgkin lymphoma), SR (human pleural effusion lymphoblast). IHC-P: Human tissues: tonsil, and anaplastic large cell lymphoma; HDLM-2. ICC/IF: KARPAS-299. Flow cyt. intr.: KARPAS-299, peripheral blood mononuclear cell (PBMC). IP: KARPAS-299.

  • 特記事項

    ab302568 does not react in WB and IHC-P with mouse and rat species. 

     

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab302568の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ICC/IF
1/500.
IP
1/30.
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cyt (Intra)
1/500.
WB
1/1000. Detects a band of approximately 18 kDa (predicted molecular weight: 14 kDa).
特記事項
ICC/IF
1/500.
IP
1/30.
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cyt (Intra)
1/500.
WB
1/1000. Detects a band of approximately 18 kDa (predicted molecular weight: 14 kDa).

ターゲット情報

  • 関連性

    BATF3 belongs to the bZIP family and contains 1 bZIP domain. Functions as a negative regulator of AP-1-mediated transcription by heterodimerizing with JUN and binding DNA at 12-O-tetradecanoylphorbol-13-acetate response elements (TRE) (consensus: 5'-TGA[CG]TCA-3'). Represses IL2 and MMP1 promoter activities.

  • 細胞内局在

    Nuclear

  • 参照データベース

    • 別名

      • 21 kD small nuclear factor isolated from T cells antibody
      • 21 kDa small nuclear factor isolated from T-cells antibody
      • ATF like 3 antibody
      • B-ATF-3 antibody
      • basic leucine zipper transcription factor, ATF-like 3 antibody
      • BATF3 antibody
      • FLJ36352 antibody
      • FLJ37535 antibody
      • hCG 39202 antibody
      • JDP1 antibody
      • Jun dimerization protein 1 antibody
      • Jun dimerization protein p21SNFT antibody
      • JUNDM1 antibody
      • SNFT antibody
      see all

    画像

    • Western blot - Anti-BATF3 antibody [EPR26558-38] (AB302568)
      Western blot - Anti-BATF3 antibody [EPR26558-38] (AB302568)
      All lanes : Anti-BATF3 antibody [EPR26558-38] (ab302568) at 1/1000 dilution

      Lane 1 : KARPAS-299 (human T cell lymphoma cell) whole cell lysate
      Lane 2 : HDLM-2 (human Hodgkin lymphoma) whole cell lysate
      Lane 3 : SR (human pleural effusion lymphoblast) whole cell lysate
      Lane 4 : Ramos (human Burkitt's lymphoma b lymphocyte) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

      Predicted band size: 14 kDa
      Observed band size: 18 kDa why is the actual band size different from the predicted?


      Exposure time: 81 seconds


      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Negative control: Ramos (PMID:34202976).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BATF3 antibody [EPR26558-38] (AB302568)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BATF3 antibody [EPR26558-38] (AB302568)

      Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling BATF3 with ab302568 at 1/500 dilution (0.942 µg/mL) followed by a ready to use Leica DS9800 (BOND™ Polymer Refine Detection kit). Positive staining in human tonsil (PMID: 28878352). The section was incubated with ab302568 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Leica DS9800 (BOND™ Polymer Refine Detection kit).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins was used.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BATF3 antibody [EPR26558-38] (AB302568)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BATF3 antibody [EPR26558-38] (AB302568)

      Immunohistochemical analysis of paraffin-embedded human Hodgkin's lymphoma tissue labeling BATF3 with ab302568 at 1/500 dilution (0.942 µg/mL) followed by a ready to use Leica DS9800 (BOND™ Polymer Refine Detection kit). Positive staining in human Hodgkin's lymphoma (PMID: 34202976, PMID:28878352). The section was incubated with ab302568 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Leica DS9800 (BOND™ Polymer Refine Detection kit).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins was used.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BATF3 antibody [EPR26558-38] (AB302568)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BATF3 antibody [EPR26558-38] (AB302568)

      Immunohistochemical analysis of paraffin-embedded human anaplastic large cell lymphoma tissue labeling BATF3 with ab302568 at 1/500 dilution (0.942 µg/mL) followed by a ready to use Leica DS9800 (BOND™ Polymer Refine Detection kit). Positive staining in human anaplastic large cell lymphoma (PMID: 34202976). The section was incubated with ab302568 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Leica DS9800 (BOND™ Polymer Refine Detection kit).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins was used. 

    • Immunocytochemistry/ Immunofluorescence - Anti-BATF3 antibody [EPR26558-38] (ab302568)
      Immunocytochemistry/ Immunofluorescence - Anti-BATF3 antibody [EPR26558-38] (ab302568)

      Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized KARPAS-299 (human anaplastic large cell lymphoma) cells labeling BATF3 with AB302568 at 1/500 dilution (0.942 µg/mL), followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preabsorbed antibody at 1/1000 dilution (2 µg/mL) (Green). Confocal image showing nuclear staining in KARPAS-299 cell line. Negative control: Ramos no staining is observed (PMID: 34202976). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (2.5 µg/mL) (Red). The Nuclear counterstain was DAPI (Blue).

      Secondary antibody control: Primary diluent was used instead of primary antibody, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preabsorbed at 1/1000 dilution (2 µg/mL).

    • Flow Cytometry (Intracellular) - Anti-BATF3 antibody [EPR26558-38] (AB302568)
      Flow Cytometry (Intracellular) - Anti-BATF3 antibody [EPR26558-38] (AB302568)

      Intracellular flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized Ramos (human Burkitt's lymphoma B lymphocyte, Left) / KARPAS-299 (human anaplastic large cell lymphoma, Right) cells labeling BATF3 with AB302568 at 1/500 dilution (0.1 µg) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody. Negative control: Ramos (PMID: 34202976).

    • Flow Cytometry (Intracellular) - Anti-BATF3 antibody [EPR26558-38] (AB302568)
      Flow Cytometry (Intracellular) - Anti-BATF3 antibody [EPR26558-38] (AB302568)

      Intracellular flow cytometric analysis of 2% paraformaldehyde fixed, 0.1% Tween-20 permeabilized human peripheral blood mononuclear cell (PBMC) cells labeling BATF3 with AB302568 at 1/500 dilution (0.1 µg)/ Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody. Cells were stained with anti-CD11c conjugated to Alexa Fluor® 647. Cells were fixed with 2% PFA for 10 min followed by intracellularly staining with rabbit IgG or ab302568.

    • Immunoprecipitation - Anti-BATF3 antibody [EPR26558-38] (AB302568)
      Immunoprecipitation - Anti-BATF3 antibody [EPR26558-38] (AB302568)

      BATF3 was immunoprecipitated from 0.35 mg KARPAS-299 (human T cell lymphoma cell) whole cell lysate with AB302568 at 1/30 dilution (2 µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using AB302568 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

      Lane 1: KARPAS-299 (human T cell lymphoma cell) whole cell lysate 10 µg (Inset)

      Lane 2: AB302568 IP in KARPAS-299 whole cell lysate

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of AB302568 in KARPAS-299 whole cell lysate

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 32 seconds

      Observed MW (kDa): 18. 

    • Anti-BATF3 antibody [EPR26558-38] (AB302568)
      Anti-BATF3 antibody [EPR26558-38] (AB302568)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab302568 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab302568 は論文での使用が確認できていません。

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