Anti-Bassoon/BSN 抗体 [SAP7F407] (ab82958)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bassoon/BSN antibody [SAP7F407] (ab82958)](https://www.abcam.co.jp/ps/products/82/ab82958/Images/ab82958-209060-anti-bassoon-antibody-sap7f407-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bassoon/BSN antibody [SAP7F407] (ab82958)")
Key features and details
- Mouse monoclonal [SAP7F407] to Bassoon/BSN
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG2a
製品の概要
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製品名
Anti-Bassoon/BSN antibody [SAP7F407]
Bassoon/BSN 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [SAP7F407] to Bassoon/BSN -
由来種
Mouse -
アプリケーション
適用あり: WB, ICC/IF, IHC-Pmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- Rat brain tissue extract This antibody gave a positive result in IHC in the following FFPE tissue: Human normal cerebral cortex.
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特記事項
This product was changed from ascites to tissue culture supernatant on 22nd May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.2
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
精製度
Tissue culture supernatant -
特記事項（精製）
Purified from TCS. -
ポリ/モノ
モノクローナル -
クローン名
SAP7F407 -
アイソタイプ
IgG2a -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab82958の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use at an assay dependent concentration. Predicted molecular weight: 416 kDa.
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| ICC/IF | (2) |
Use at an assay dependent concentration.
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| IHC-P | (8) |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 特記事項 |
|---|
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WB
Use at an assay dependent concentration. Predicted molecular weight: 416 kDa. |
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ICC/IF
Use at an assay dependent concentration. |
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IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Is thought to be involved in the organization of the cytomatrix at the nerve terminals active zone (CAZ) which regulates neurotransmitter release. Seems to act through binding to ERC2/CAST1. Essential in regulated neurotransmitter release from a subset of brain glutamatergic synapses. Involved in the formation of the retinal photoreceptor ribbon synapses. -
組織特異性
Exclusively expressed in brain. -
翻訳後修飾
Myristoylated. The N-terminal myristoylation is not sufficient for presynaptic localization. -
細胞内局在
Cytoplasm. Cell junction > synapse > synaptosome. Cytoplasm > cytoskeleton. Localized to the active zone of presynaptic density. - Information by UniProt
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参照データベース
- Entrez Gene: 8927 Human
- Entrez Gene: 12217 Mouse
- Entrez Gene: 29138 Rat
- Omim: 604020 Human
- SwissProt: Q9UPA5 Human
- SwissProt: O88737 Mouse
- SwissProt: O88778 Rat
- Unigene: 194684 Human
see all -
別名
- Bassoon protein antibody
- bsn antibody
- BSN_HUMAN antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bassoon/BSN antibody [SAP7F407] (ab82958)This image is courtesy of an abreview submitted by Carl Hobbs, Kings's College London, United Kingdom.
IHC-P image of Bassoon/BSN staining on mouse cerebellum sections using ab82958 (1:1500). The sections were deparaffinized and subjected to heat mediated antigen retrieval using HEIR. The sections were blocked using 1% BSA for 10 mins at 21°C. ab82958 was diluted 1:1500 and incubated with the sctions for 2 hours at 21°C. The secondary antibody used was goat polyclonal to mouse IgG conjugated to Biotin (1:200).
This image was generated using the ascites version of the product.
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![Western blot - Anti-Bassoon/BSN antibody [SAP7F407] (ab82958)](https://www.abcam.co.jp/ps/products/82/ab82958/Images/ab82958-97687-anti-bassoon-antibody-sap7f407-western-blot.jpg)
Western blot - Anti-Bassoon/BSN antibody [SAP7F407] (ab82958)Anti-Bassoon/BSN antibody [SAP7F407] (ab82958) at 1/500 dilution + rat brain tissue extract
Developed using the ECL technique.
Predicted band size: 416 kDaThis image was generated using the ascites version of the product.
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![Immunocytochemistry/ Immunofluorescence - Anti-Bassoon/BSN antibody [SAP7F407] (ab82958)](https://www.abcam.co.jp/ps/products/82/ab82958/Images/ab82958-97686-anti-bassoon-antibody-sap7f407-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Bassoon/BSN antibody [SAP7F407] (ab82958)Bassoon/BSN localization in the rat brain cerebellar molecular layer using Bassoon/BSN monoclonal antibody (ab82958).
This image was generated using the ascites version of the product.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bassoon/BSN antibody [SAP7F407] (ab82958)](https://www.abcam.co.jp/ps/products/82/ab82958/Images/ab82958-207281-anti-bassoon-antibody-sap7f407-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bassoon/BSN antibody [SAP7F407] (ab82958)This image is courtesy of an abreview submitted by Carl Hobbs, Kings's College London, United Kingdom.IHC-P image of Bassoon/BSN staining on rat cerebellum sections using ab82958 (1:1000). The sections were deparaffinized and subjected to heat mediated antigen retrieval using citric acid. The sections were blocked using 1% BSA for 10 mins at 21°C. ab82958 was diluted 1:1000 and incubated with the sections for 2 hours at 21°C. The secondary antibody used was goat polyclonal to mouse IgG conjugated to biotin (1:200).
This image was generated using the ascites version of the product.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bassoon/BSN antibody [SAP7F407] (ab82958)](https://www.abcam.co.jp/ps/products/82/ab82958/Images/ab82958-205109-anti-bassoon-antibody-sap7f407-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bassoon/BSN antibody [SAP7F407] (ab82958)IHC image of Bassoon/BSN staining in Human normal cerebral cortex formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab82958, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
This image was generated using the ascites version of the product.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (68)
ab82958 は 68 報の論文で使用されています。
- Zhang C et al. The synaptic basis of activity-dependent eye-specific competition. Cell Rep 42:112085 (2023). PubMed: 36753422
- Masato A et al. DOPAL initiates αSynuclein-dependent impaired proteostasis and degeneration of neuronal projections in Parkinson's disease. NPJ Parkinsons Dis 9:42 (2023). PubMed: 36966140
- Tran KM et al. A Trem2R47H mouse model without cryptic splicing drives age- and disease-dependent tissue damage and synaptic loss in response to plaques. Mol Neurodegener 18:12 (2023). PubMed: 36803190
- Strong CE et al. Functional brain region-specific neural spheroids for modeling neurological diseases and therapeutics screening. Commun Biol 6:1211 (2023). PubMed: 38017066
- Aggarwal A et al. Glutamate indicators with improved activation kinetics and localization for imaging synaptic transmission. Nat Methods 20:925-934 (2023). PubMed: 37142767