Anti-basal cell Cytokeratin 抗体 [RCK103] (ab9222)
Key features and details
- Mouse monoclonal [RCK103] to basal cell Cytokeratin
- Suitable for: Flow Cyt, IHC-Fr
- Reacts with: Dog, Human, Zebrafish
- Isotype: IgG1
製品の概要
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製品名
Anti-basal cell Cytokeratin antibody [RCK103] -
製品の詳細
Mouse monoclonal [RCK103] to basal cell Cytokeratin -
由来種
Mouse -
特異性
This antibody recognizes (amongst others) cytokeratin 5. It stains basal cells in combined and stratified epithelial tissues. It also recognizes the stem cell population, including the so-called amplifying cells in the prostate epithelium.
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アプリケーション
適用あり: Flow Cyt, IHC-Frmore details -
種交差性
交差種: Dog, Human, Zebrafish -
免疫原
Tissue, cells or virus corresponding to Human basal cell Cytokeratin. Mix of cell preparations containing human cytokeratins.
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特記事項
Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 – 7.8. The individual human cytokeratins are designated 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading...
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精製度
Protein G purified -
一次抗体 備考
Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 – 7.8. The individual human cytokeratins are designated 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays. -
ポリ/モノ
モノクローナル -
クローン名
RCK103 -
ミエローマ
Sp2/0-Ag14 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab9222の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Flow Cyt |
Use 1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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IHC-Fr |
1/100 - 1/200.
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特記事項 |
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Flow Cyt
Use 1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
IHC-Fr
1/100 - 1/200. |
画像
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Indirect immunofluorescence staining of frozen section of dog skin ab9222 at dilution 1/100 showing positive staining in basal cells of epidermis.
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Immunohistochemistry on frozen sections of human skin
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Overlay histogram showing A431 cells stained with ab9222 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab9222, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in A431 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
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Immunofluorescence staining of a 7 days old zebrafish embryo, showing positive reactivity in epithelia lining the intestine and internal organs
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab9222 は 4 報の論文で使用されています。
- Tamberg N et al. Keap1-MCM3 interaction is a potential coordinator of molecular machineries of antioxidant response and genomic DNA replication in metazoa. Sci Rep 8:12136 (2018). PubMed: 30108253
- Kuzinski J et al. Modulation of vH+-ATPase is part of the functional adaptation of sheep rumen epithelium to high-energy diet. Am J Physiol Regul Integr Comp Physiol 303:R909-20 (2012). Flow Cyt ; Sheep . PubMed: 22972839
- Halkidou K et al. Upregulation and nuclear recruitment of HDAC1 in hormone refractory prostate cancer. Prostate 59:177-89 (2004). PubMed: 15042618
- Verhagen AP et al. Differential expression of keratins in the basal and luminal compartments of rat prostatic epithelium during degeneration and regeneration. Prostate 13:25-38 (1988). PubMed: 2458583