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AB50984

Anti-BARD1 抗体 [2059C4a]

Anti-BARD1 antibody [2059C4a]

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(4 Publications)

Mouse Monoclonal BARD1 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human BARD1.

別名を表示する

BRCA1-associated RING domain protein 1, BARD-1, RING-type E3 ubiquitin transferase BARD1, BARD1

2 Images
Western blot - Anti-BARD1 antibody [2059C4a] (AB50984)
  • WB

Unknown

Western blot - Anti-BARD1 antibody [2059C4a] (AB50984)

All lanes:

Western blot - Anti-BARD1 antibody [2059C4a] (ab50984) at 1/50 dilution

All lanes:

HEK293 whole cell lysate at 50 µg

Secondary

All lanes:

Anti mouse IgG antibody at 1/2500 dilution

Predicted band size: 87 kDa

Observed band size: 100 kDa

false

Immunoprecipitation - Anti-BARD1 antibody [2059C4a] (AB50984)
  • IP

Unknown

Immunoprecipitation - Anti-BARD1 antibody [2059C4a] (AB50984)

ab50984 at 1/50 dilution precipitating RIPA lysate of HeLa cells at 500μg; visualised with anti mouse IgG antibody at 1/2500 diltuion.

All lanes:

Immunoprecipitation - Anti-BARD1 antibody [2059C4a] (ab50984)

Predicted band size: 87 kDa

false

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

2059C4a

アイソタイプ

IgG1

キャリアフリー

No

交差種

Human

アプリケーション

WB, IP

applications

免疫原

Recombinant Fragment Protein within Human BARD1. The exact immunogen used to generate this antibody is proprietary information.

Q99728

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/50", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/50", "WB-species-notes": "<p></p>" } } }
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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein G
精製に関する特記事項
Protein G purified from culture of supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (~95%) fetal bovine serum. Filtered through a 0.22 µm membrane.
バッファー組成
pH: 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 1% BSA
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

E3 ubiquitin-protein ligase. The BRCA1-BARD1 heterodimer specifically mediates the formation of 'Lys-6'-linked polyubiquitin chains and coordinates a diverse range of cellular pathways such as DNA damage repair, ubiquitination and transcriptional regulation to maintain genomic stability. Plays a central role in the control of the cell cycle in response to DNA damage. Acts by mediating ubiquitin E3 ligase activity that is required for its tumor suppressor function. Also forms a heterodimer with CSTF1/CSTF-50 to modulate mRNA processing and RNAP II stability by inhibiting pre-mRNA 3' cleavage.
See full target information BARD1
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文献 (4)

Recent publications for all applications. Explore the full list and refine your search

The EMBO journal 42:e113565 PubMed37305927

2023

BRCA1/BARD1 intrinsically disordered regions facilitate chromatin recruitment and ubiquitylation.

Applications

Unspecified application

Species

Unspecified reactive species

Samuel R Witus,Lisa M Tuttle,Wenjing Li,Alex Zelter,Meiling Wang,Klaiten E Kermoade,Damien B Wilburn,Trisha N Davis,Peter S Brzovic,Weixing Zhao,Rachel E Klevit

Cancer research communications 2:220-232 PubMed36187937

2022

BRCA1-associated RING domain-1 (BARD1) loss and GBP1 expression enhance sensitivity to DNA damage in Ewing sarcoma.

Applications

Unspecified application

Species

Unspecified reactive species

Lisa M Maurer,Jessica D Daley,Elina Mukherjee,Rosemarie E Venier,Claire M Julian,Nathanael G Bailey,Michelle F Jacobs,Chandan Kumar-Sinha,Haley Raphael,Nivitha Periyapatna,Kurt Weiss,Katherine A Janeway,Rajen Mody,Peter C Lucas,Linda M McAllister-Lucas,Kelly M Bailey

Cell biology international 46:922-932 PubMed35143098

2022

USP25 inhibits DNA damage by stabilizing BARD1 protein in a house dust mite-induced asthmatic model in vitro and in vivo.

Applications

Unspecified application

Species

Unspecified reactive species

Cong Gan,Ye Wang,Qian Zhao,Mi Kong,Jinnan Chen,Wanying Zhang,Lingxiao Tan,Man Tian

Proceedings of the National Academy of Sciences of 109:13650-5 PubMed22869732

2012

BRCA1 and HSP90 cooperate in homologous and non-homologous DNA double-strand-break repair and G2/M checkpoint activation.

Applications

WB

Species

Unspecified reactive species

Shane R Stecklein,Easwari Kumaraswamy,Fariba Behbod,Wenjia Wang,Vamsee Chaguturu,Lisa M Harlan-Williams,Roy A Jensen
View all publications
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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。
保証に関する詳細については利用規約をご確認ください。
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