Anti-BAP1 抗体 (ab167250)
Key features and details
- Mouse polyclonal to BAP1
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-BAP1 antibody
BAP1 一次抗体 製品一覧 -
製品の詳細
Mouse polyclonal to BAP1 -
由来種
Mouse -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Human -
免疫原
Recombinant full length protein corresponding to amino acids 1-729 of Human BAP1 (NP_004647.1).
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ポジティブ･コントロール
- BAP1 transfected 293T cell lysate; HeLa cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.4
Constituent: 99% PBS -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab167250の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 80 kDa.
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| ICC/IF |
Use a concentration of 10 µg/ml.
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| 特記事項 |
|---|
|
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 80 kDa. |
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ICC/IF
Use a concentration of 10 µg/ml. |
ターゲット情報
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機能
Deubiquitinating enzyme that plays a key role in chromatin by mediating deubiquitination of histone H2A and HCFC1. Catalytic component of the PR-DUB complex, a complex that specifically mediates deubiquitination of histone H2A monoubiquitinated at 'Lys-119' (H2AK119ub1). Does not deubiquitinate monoubiquitinated histone H2B. Acts as a regulator of cell growth by mediating deubiquitination of HCFC1 N-terminal and C-terminal chains, with some specificity toward 'Lys-48'-linked polyubiquitin chains compared to 'Lys-63'-linked polyubiquitin chains. Deubiquitination of HCFC1 does not lead to increase stability of HCFC1. Interferes with the BRCA1 and BARD1 heterodimer activity by inhibiting their ability to mediate ubiquitination and autoubiquitination. It however does not mediate deubiquitination of BRCA1 and BARD1. Acts as a tumor suppressor. -
組織特異性
Highly expressed in testis, placenta and ovary. Expressed in breast. -
配列類似性
Belongs to the peptidase C12 family. BAP1 subfamily. -
翻訳後修飾
Phosphorylated upon DNA damage, probably by ATM or ATR. -
細胞内局在
Cytoplasm. Nucleus. Mainly nuclear. Binds to chromatin. - Information by UniProt
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参照データベース
- Entrez Gene: 8314 Human
- Omim: 603089 Human
- SwissProt: Q92560 Human
- Unigene: 106674 Human
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別名
- BAP 1 antibody
- Bap1 antibody
- BAP1_HUMAN antibody
see all
画像
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Western blot - Anti-BAP1 antibody (ab167250)All lanes : Anti-BAP1 antibody (ab167250) at 1 µg
Lane 1 : BAP1 transfected 293T cell lysate
Lane 2 : Non-transfected lysate
Lysates/proteins at 15 µl per lane.
Developed using the ECL technique.
Predicted band size: 80 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-BAP1 antibody (ab167250)Immunofluorescence analysis of HeLa cells labeling BAP1 with ab167250 at 10µg/mL.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (1)
ab167250 は 1 報の論文で使用されています。
- Sahtoe DD et al. BAP1/ASXL1 recruitment and activation for H2A deubiquitination. Nat Commun 7:10292 (2016). PubMed: 26739236
