AB229190

Anti-BANK1 抗体 [EPR20788]

Anti-BANK1 antibody [EPR20788]

RabMAb
Recombinant
詳細を見る

Be the first to review this product! Submit a review

(1 Publication)

Rabbit Recombinant Monoclonal BANK1 antibody. Suitable for Flow Cyt (Intra), IHC-P and reacts with Human samples. Cited in 1 publication.

別名を表示する

B-cell scaffold protein with ankyrin repeats, BANK1

3 Images

Flow Cytometry (Intracellular) - Anti-BANK1 antibody [EPR20788] (AB229190)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-BANK1 antibody [EPR20788] (AB229190)

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized Jurkat (Human T cell leukemia T lymphocyte, Left panel) / Daudi (human Burkitt's lymphoma lymphoblast, Right panel) cell lines labeling BANK1 with ab229190 at 1/400 (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti rabbit IgG (Alexa Fluor^ï¿½ 488, ab150077), at 1/2000 dilution was used as the secondary antibody.

The Jurkat cell line is a negative control for BANK1.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BANK1 antibody [EPR20788] (AB229190)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BANK1 antibody [EPR20788] (AB229190)

Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling BANK1 with ab229190 at 1/4000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining is weak in the germinal center but strong in the mantle zones of human tonsil (PMID : 24879116). Counter stained with Hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BANK1 antibody [EPR20788] (AB229190)

Immunohistochemical analysis of paraffin-embedded human Large B cell lymphoma tissue labeling BANK1 with ab229190 at 1/4000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining in human Large B Cell Lymphoma cells (PMID : 24879116) is observed. Counter stained with Hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR20788

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

Flow Cyt (Intra), IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/400", "FlowCytIntra-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/4000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }

style

left-column

製品の詳細

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

style

left-column

style

left-column

style

left-column

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

ターゲットの情報

Involved in B-cell receptor (BCR)-induced Ca(2+) mobilization from intracellular stores. Promotes Lyn-mediated phosphorylation of IP3 receptors 1 and 2.

See full target information BANK1

style

left-column

文献 (1)

Recent publications for all applications. Explore the full list and refine your search

The FEBS journal 291:5214-5235 PubMed39434428

2024

TBK1 is involved in M-CSF-induced macrophage polarization through mediating the IRF5/IRF4 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Yuanyuan Li,Le Ji,Chang Liu,Juanjuan Li,Di Wen,Zhongyao Li,Lishuang Yu,Moran Guo,Shaoran Zhang,Weisong Duan,Le Yi,Yue Bi,Hui Bu,Chunyan Li,Yakun Liu

View all publications

style

left-column

style

left-column

style

right-column

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-BANK1 抗体 [EPR20788]

Anti-BANK1 antibody [EPR20788]

Flow Cytometry (Intracellular) - Anti-BANK1 antibody [EPR20788] (AB229190)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BANK1 antibody [EPR20788] (AB229190)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BANK1 antibody [EPR20788] (AB229190)

関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

製品プロトコール

ターゲットの情報

文献 (1)

代替製品

Primary Antibodies

Primary Antibodies

組合せ製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise