Anti-Bak 抗体 (ab69404)
Key features and details
- Rabbit polyclonal to Bak
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Bak antibody
Bak 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Bak -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IF, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide derived from the sequence of human Bak, conjugated to KLH.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab69404の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (1) |
1/1000. Detects a band of approximately 28 kDa (predicted molecular weight: 23 kDa).
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| ICC/IF |
Use at an assay dependent concentration.
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| IHC-P |
1/50.
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| 特記事項 |
|---|
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WB
1/1000. Detects a band of approximately 28 kDa (predicted molecular weight: 23 kDa). |
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ICC/IF
Use at an assay dependent concentration. |
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IHC-P
1/50. |
ターゲット情報
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機能
In the presence of an appropriate stimulus, accelerates programmed cell death by binding to, and antagonizing the anti-apoptotic action of BCL2 or its adenovirus homolog E1B 19k protein. Low micromolar levels of zinc ions inhibit the promotion of apoptosis. -
組織特異性
Expressed in a wide variety of tissues, with highest levels in the heart and skeletal muscle. -
配列類似性
Belongs to the Bcl-2 family. -
ドメイン
Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family. -
細胞内局在
Mitochondrion membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 578 Human
- Omim: 600516 Human
- SwissProt: Q16611 Human
- Unigene: 485139 Human
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別名
- Apoptosis regulator BAK antibody
- BAK antibody
- BAK like antibody
see all
画像
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Western blot - Anti-Bak antibody (ab69404)Anti-Bak antibody (ab69404) at 1/1000 dilution + A431 cell lysate
Predicted band size: 23 kDa
Observed band size: 28 kDa why is the actual band size different from the predicted? -
Immunocytochemistry/ Immunofluorescence - Anti-Bak antibody (ab69404)Immunofluorescent staining of HeLa cells using ab69404. nuclei were counter-stained using DRAQ5. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bak antibody (ab69404)Immunohistochemical analysis of human cervical cancer tissue using 1/50 ab69404.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab69404 は 5 報の論文で使用されています。
- Quinlan S et al. Canine Papillomavirus 2 E6 Does Not Interfere With UVB-Induced Upregulation of p53 and p53-Regulated Genes. Front Vet Sci 8:570982 (2021). PubMed: 33748203
- Feng Q et al. Intracellular expression of arginine deiminase activates the mitochondrial apoptosis pathway by inhibiting cytosolic ferritin and inducing chromatin autophagy. BMC Cancer 20:665 (2020). PubMed: 32677906
- Yuan C et al. BNIP3/Bcl-2-mediated apoptosis induced by cyclic tensile stretch in human cartilage endplate-derived stem cells. Exp Ther Med 15:235-241 (2018). PubMed: 29375685
- Li Z et al. Inhibition of neuroblastoma proliferation by PF-3758309, a small-molecule inhibitor that targets p21-activated kinase 4. Oncol Rep 38:2705-2716 (2017). PubMed: 29048629
- Chen G et al. Suppression of Sirt1 sensitizes lung cancer cells to WEE1 inhibitor MK-1775-induced DNA damage and apoptosis. Oncogene 36:6863-6872 (2017). PubMed: 28869605