AB69404

Anti-Bak 抗体

Anti-Bak antibody

(1 Review)

(6 Publications)

Rabbit Polyclonal BAK antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human BAK1 conjugated to Keyhole Limpet Haemocyanin.

別名を表示する

BAK, BCL2L7, CDN1, BAK1, Bcl-2 homologous antagonist/killer, Apoptosis regulator BAK, Bcl-2-like protein 7, Bcl2-L-7

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bak antibody (AB69404)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bak antibody (AB69404)

Immunohistochemical analysis of human cervical cancer tissue using 1/50 ab69404.

Immunocytochemistry/ Immunofluorescence - Anti-Bak antibody (AB69404)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Bak antibody (AB69404)

Immunofluorescent staining of HeLa cells using ab69404. nuclei were counter-stained using DRAQ5.

Unknown

Western blot - Anti-Bak antibody (AB69404)

All lanes:

Western blot - Anti-Bak antibody (ab69404) at 1/1000 dilution

All lanes:

A431 cell lysate

Predicted band size: 23 kDa

Observed band size: 28 kDa

false

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

IHC-P, WB, ICC/IF

applications

免疫原

Synthetic Peptide within Human BAK1 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q16611

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

style

left-column

style

left-column

出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

バッファー組成

Preservative: 0.09% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

style

left-column

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

The Bak protein also known as Bcl-2 homologous antagonist killer plays a mechanical role in the mitochondrial apoptosis pathway. It often functions as a pro-apoptotic member of the Bcl-2 protein family. Bak is expressed widely in the body being present in tissues such as the brain and heart. The molecular weight of Bak is approximately 23 kilodaltons. Bak interacts with other mitochondrial proteins to trigger cell death processes.

Biological function summary

Bak engages in promoting apoptosis by disrupting the mitochondrial outer membrane potential. It is part of the Bcl-2 family complex which balances cell survival and cell death. Bak collaborates with Bax protein to form pores in the mitochondrial membrane releasing cytochrome c and other apoptotic factors. This process initiates the cascade of caspases leading to programmed cell death.

Pathways

Bak is a critical component of the intrinsic apoptotic pathway. It directly interacts with pro-apoptotic proteins like Bax and anti-apoptotic proteins such as Bcl-2 and Bcl-xL. Bak's action in the apoptotic pathway involves a delicate balance between survival and death signals affecting cellular homeostasis. In addition Bak's interaction with the p53 pathway emphasizes its role in response to DNA damage ensuring damaged cells do not proliferate uncontrollably.

Malfunction or dysregulation of Bak can lead to cancer and neurodegenerative disorders. In cancer reduced Bak activity may allow cells to evade apoptosis promoting tumor survival and growth. Conversely in diseases like Parkinson's excessive Bak activity may result in enhanced neuronal apoptosis. Bak's interaction with proteins such as Bcl-2 also makes it a potential target for therapies aimed at restoring apoptotic balance in affected cells.

style

left-column

style

left-column

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

ターゲットの情報

Plays a role in the mitochondrial apoptotic process. Upon arrival of cell death signals, promotes mitochondrial outer membrane (MOM) permeabilization by oligomerizing to form pores within the MOM. This releases apoptogenic factors into the cytosol, including cytochrome c, promoting the activation of caspase 9 which in turn processes and activates the effector caspases.

See full target information BAK1

style

left-column

文献 (6)

Recent publications for all applications. Explore the full list and refine your search

Blood cancer journal 11:111 PubMed34099621

2021

The combination of CUDC-907 and gilteritinib shows promising in vitro and in vivo antileukemic activity against FLT3-ITD AML.

Applications

Unspecified application

Species

Unspecified reactive species

Xinan Qiao,Jun Ma,Tristan Knight,Yongwei Su,Holly Edwards,Lisa Polin,Jing Li,Juiwanna Kushner,Sijana H Dzinic,Kathryn White,Jian Wang,Hai Lin,Yue Wang,Liping Wang,Guan Wang,Jeffrey W Taub,Yubin Ge

Frontiers in veterinary science 8:570982 PubMed33748203

2021

Canine Papillomavirus 2 E6 Does Not Interfere With UVB-Induced Upregulation of p53 and p53-Regulated Genes.

Applications

Unspecified application

Species

Unspecified reactive species

Sarah Quinlan,Susan May,Ryan Weeks,Hang Yuan,Jennifer Luff

BMC cancer 20:665 PubMed32677906

2020

Intracellular expression of arginine deiminase activates the mitochondrial apoptosis pathway by inhibiting cytosolic ferritin and inducing chromatin autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Qingyuan Feng,Xuzhao Bian,Xuan Liu,Ying Wang,Huiting Zhou,Xiaojing Ma,Chunju Quan,Yi Yao,Zhongliang Zheng

Experimental and therapeutic medicine 15:235-241 PubMed29375685

2018

BNIP3/Bcl-2-mediated apoptosis induced by cyclic tensile stretch in human cartilage endplate-derived stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Chao Yuan,Luqiao Pu,Zhiliang He,Jian Wang

Oncology reports 38:2705-2716 PubMed29048629

2017

Inhibition of neuroblastoma proliferation by PF-3758309, a small-molecule inhibitor that targets p21-activated kinase 4.

Applications

Unspecified application

Species

Unspecified reactive species

Zhiheng Li,Xiaolu Li,Lixiao Xu,Yanfang Tao,Chun Yang,Xiaolan Chen,Fang Fang,Yi Wu,Xin Ding,He Zhao,Mei Li,Guanghui Qian,Yunyun Xu,Junli Ren,Weiwei Du,Jian Wang,Jun Lu,Shaoyan Hu,Jian Pan

Oncogene 36:6863-6872 PubMed28869605

2017

Suppression of Sirt1 sensitizes lung cancer cells to WEE1 inhibitor MK-1775-induced DNA damage and apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

G Chen,B Zhang,H Xu,Y Sun,Y Shi,Y Luo,H Jia,F Wang

View all publications

style

left-column

style

left-column

style

right-column

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Bak 抗体

Anti-Bak antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bak antibody (AB69404)

Immunocytochemistry/ Immunofluorescence - Anti-Bak antibody (AB69404)

Western blot - Anti-Bak antibody (AB69404)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (6)

代替製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

組合せ製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Abcam product promise