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リコンビナントRabMAb

Anti-Bag3 抗体 [EPR20207-58-4] (ab246225)

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Immunocytochemistry/ Immunofluorescence - Anti-Bag3 antibody [EPR20207-58-4] (ab246225)
  • Western blot - Anti-Bag3 antibody [EPR20207-58-4] (ab246225)
  • Flow Cytometry (Intracellular) - Anti-Bag3 antibody [EPR20207-58-4] (ab246225)
  • Anti-Bag3 antibody [EPR20207-58-4] (ab246225)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20207-58-4] to Bag3
  • Suitable for: WB, ICC/IF, Flow Cyt (Intra)
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-Bag3 antibody [EPR20207-58-4]
    Bag3 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR20207-58-4] to Bag3

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, ICC/IF, Flow Cyt (Intra)more details
    適用なし: IHC-P or IP

  • 種交差性

    交差種: Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Human skeletal muscle lysate; HeLa whole cell lysate. ICC/IF: HeLa cells. Flow Cyt (intra): HeLa cells.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab246225の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/1000. Detects a band of approximately 80 kDa (predicted molecular weight: 62 kDa).
ICC/IF
1/70.
Flow Cyt (Intra)
1/70.
特記事項
WB
1/1000. Detects a band of approximately 80 kDa (predicted molecular weight: 62 kDa).
ICC/IF
1/70.
Flow Cyt (Intra)
1/70.
追加情報
Is unsuitable for IHC-P or IP.

ターゲット情報

  • 機能

    Inhibits the chaperone activity of HSP70/HSC70 by promoting substrate release. Has anti-apoptotic activity.

  • 関連疾患

    Defects in BAG3 are the cause of myopathy myofibrillar BAG3-related (MFM-BAG3) [MIM:612954]. A neuromuscular disorder that results in early-onset, severe, progressive, diffuse muscle weakness associated with cardiomyopathy, severe respiratory insufficiency during adolescence, and a rigid spine in some patients. At ultrastructural level, muscle fibers display structural alterations consisting of replacement of the normal myofibrillar markings by small, dense granules, or larger hyaline masses, or amorphous material.

  • 配列類似性

    Contains 1 BAG domain.
    Contains 2 WW domains.
  • Information by UniProt

  • 参照データベース

    • 別名

      • BAG 3 antibody
      • BAG family molecular chaperone regulator 3 antibody
      • BAG-3 antibody
      • Bag3 antibody
      • BAG3_HUMAN antibody
      • Bcl 2 binding protein antibody
      • Bcl-2-associated athanogene 3 antibody
      • Bcl-2-binding protein Bis antibody
      • BCL2 associated athanogene 3 antibody
      • BCL2 binding athanogene 3 antibody
      • BIS antibody
      • CAIR 1 antibody
      • Docking protein CAIR 1 antibody
      • Docking protein CAIR-1 antibody
      • MFM6 antibody
      see all

    画像

    • Immunocytochemistry/ Immunofluorescence - Anti-Bag3 antibody [EPR20207-58-4] (ab246225)
      Immunocytochemistry/ Immunofluorescence - Anti-Bag3 antibody [EPR20207-58-4] (ab246225)

      Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) and HL-60 (human promyelocytic leukemia cell line) cells labeling Bag3 with ab246225 at 1/70 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic and weakly nuclear staining in HeLa cell line. Negative control: HL-60 (PMID: 17974966).

      The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red).

      Control: PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    • Western blot - Anti-Bag3 antibody [EPR20207-58-4] (ab246225)
      Western blot - Anti-Bag3 antibody [EPR20207-58-4] (ab246225)
      All lanes : Anti-Bag3 antibody [EPR20207-58-4] (ab246225) at 1/1000 dilution

      Lane 1 : Human skeletal muscle lysate
      Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
      Lane 3 : HL-60 (human promyelocytic leukemia cell line) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Predicted band size: 62 kDa
      Observed band size: 80 kDa why is the actual band size different from the predicted?



      Exposure time : Lane 1: 30 seconds; Lane 2: 170 seconds; Lane 3: 180 seconds.

      Blocking and dilution buffer: 5% NFDM/TBST.

      Either Goat Anti-Rabbit IgG H&L (HRP) (ab97051) or VeriBlot for IP Detection Reagent (HRP) (ab131366), were used for detection.

      The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 17974966).

      Negative control: HL-60 (PMID: 17974966).

    • Flow Cytometry (Intracellular) - Anti-Bag3 antibody [EPR20207-58-4] (ab246225)
      Flow Cytometry (Intracellular) - Anti-Bag3 antibody [EPR20207-58-4] (ab246225)

      Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HL-60 (human promyelocytic leukemia cell line) (Left) and HeLa (human epithelial cell line from cervix adenocarcinoma) (Right) cells labeling Bag3 with ab246225 at 1/70 dilution (red) compared witha Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077), at 1/2000 dilution was used as the secondary antibody. 

      Negative control: HL-60 (PMID: 17974966). 

       

       

    • Anti-Bag3 antibody [EPR20207-58-4] (ab246225)
      Anti-Bag3 antibody [EPR20207-58-4] (ab246225)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab246225 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab246225 は論文での使用が確認できていません。

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