Anti-Bag3 抗体 (ab47124)
Key features and details
- Rabbit polyclonal to Bag3
- Suitable for: ICC/IF, IP, IHC-P, IHC-Fr, WB
- Reacts with: Mouse, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Bag3 antibody
Bag3 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Bag3 -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, IP, IHC-P, IHC-Fr, WBmore details -
種交差性
交差種: Mouse, Human -
免疫原
A recombinant protein fragment corresponding to the C terminal 196 amino acids of human BAG3.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
Preservative: 0.05% Sodium azide
Constituent: Whole serum -
Concentration information loading... -
精製度
Whole antiserum -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab47124の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
Use at an assay dependent concentration.
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| IP |
Use at an assay dependent concentration.
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| IHC-P |
1/1000 - 1/5000.
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| IHC-Fr |
Use at an assay dependent concentration.
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| WB | (1) |
1/1000 - 1/2000. Predicted molecular weight: 62 kDa.
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| 特記事項 |
|---|
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ICC/IF
Use at an assay dependent concentration. |
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IP
Use at an assay dependent concentration. |
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IHC-P
1/1000 - 1/5000. |
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IHC-Fr
Use at an assay dependent concentration. |
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WB
1/1000 - 1/2000. Predicted molecular weight: 62 kDa. |
ターゲット情報
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機能
Inhibits the chaperone activity of HSP70/HSC70 by promoting substrate release. Has anti-apoptotic activity. -
関連疾患
Defects in BAG3 are the cause of myopathy myofibrillar BAG3-related (MFM-BAG3) [MIM:612954]. A neuromuscular disorder that results in early-onset, severe, progressive, diffuse muscle weakness associated with cardiomyopathy, severe respiratory insufficiency during adolescence, and a rigid spine in some patients. At ultrastructural level, muscle fibers display structural alterations consisting of replacement of the normal myofibrillar markings by small, dense granules, or larger hyaline masses, or amorphous material. -
配列類似性
Contains 1 BAG domain.
Contains 2 WW domains. - Information by UniProt
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参照データベース
- Entrez Gene: 9531 Human
- Entrez Gene: 29810 Mouse
- Omim: 603883 Human
- SwissProt: O95817 Human
- SwissProt: Q9JLV1 Mouse
- Unigene: 523309 Human
- Unigene: 84073 Mouse
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別名
- BAG 3 antibody
- BAG family molecular chaperone regulator 3 antibody
- BAG-3 antibody
see all
画像
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Western blot - Anti-Bag3 antibody (ab47124)All lanes : Anti-Bag3 antibody (ab47124) at 1/2000 dilution
Lane 1 : Thymus lysates, total protein (20ug/lane)
Lane 2 : Spleen lysates, total protein (20ug/lane)
Lane 3 : Skeletal(quadriceps) lysates, total protein (20ug/lane)
Lane 4 : Skeletal(diaphragm) lysates, total protein (20ug/lane)
Lane 5 : Smooth (heart) muscle lysates, total protein (20ug/lane)
Predicted band size: 62 kDa
Observed band size: 86 kDa why is the actual band size different from the predicted?
Tissue lysates, normalized for total protein (20 µg/lane), were from a 4 week old male mouse. BAG3 expression was detected at highest levels in skeletal (quadriceps and diaphragm) and smooth (heart) muscle specimens. -
Immunocytochemistry/ Immunofluorescence - Anti-Bag3 antibody (ab47124)ICC/IF image of ab47124 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab47124) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Immunohistochemistry (Frozen sections) - Anti-Bag3 antibody (ab47124)Frozen mouse muscle tissue section stained for BAG3 expression using ab47124 at 1/2000. The tissue section was fixed in 3.8% paraformaldehyde prior to staining. BAG3 localizes with Z-disk proteins." -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bag3 antibody (ab47124)Immunohistochemistry of formalin fixed paraffin embedded human pancreas tissue sections using ab47124 at 1/2000. A, normal pancreas. B and C, pancreatic cancer. Hematoxylin-eosin counterstain. -
Western blot - Anti-Bag3 antibody (ab47124)Anti-Bag3 antibody (ab47124) + Hela lysate at 0.5 mg/ml
Predicted band size: 62 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-Bag3 antibody (ab47124)Immunocytochemistry/Immunofluorescence of A431 cells stained with Anti-Bag3 antibody (ab47124) green. Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (24)
ab47124 は 24 報の論文で使用されています。
- Galbiati M et al. Bicalutamide and Trehalose Ameliorate Spinal and Bulbar Muscular Atrophy Pathology in Mice. Neurotherapeutics 20:524-545 (2023). PubMed: 36717478
- Pan XW et al. Molecular subtyping and characterization of clear cell renal cell carcinoma by tumor differentiation trajectories. iScience 26:108370 (2023). PubMed: 38034348
- Piccolella M et al. Retinoic Acid Downregulates HSPB8 Gene Expression in Human Breast Cancer Cells MCF-7. Front Oncol 11:652085 (2021). PubMed: 34136389
- Lee S et al. Targeting HSF1 as a Therapeutic Strategy for Multiple Mechanisms of EGFR Inhibitor Resistance in EGFR Mutant Non-Small-Cell Lung Cancer. Cancers (Basel) 13:N/A (2021). PubMed: 34203709
- Tanboon J et al. Inflammatory features in sporadic late-onset nemaline myopathy are independent from monoclonal gammopathy. Brain Pathol 31:e12962 (2021). PubMed: 34043258