Anti-Bag3 抗体

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Bag3 antibody (AB47124)

Immunocytochemistry/Immunofluorescence of A431 cells stained with Anti-Bag3 antibody (ab47124) green. Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bag3 antibody (AB47124)

Immunohistochemistry of formalin fixed paraffin embedded human pancreas tissue sections using ab47124 at 1/2000. A, normal pancreas. B and C, pancreatic cancer. Hematoxylin-eosin counterstain.

• IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Anti-Bag3 antibody (AB47124)

Frozen mouse muscle tissue section stained for BAG3 expression using ab47124 at 1/2000. The tissue section was fixed in 3.8% paraformaldehyde prior to staining. BAG3 localizes with Z-disk proteins."

• WB

Unknown

Western blot - Anti-Bag3 antibody (AB47124)

Tissue lysates, normalized for total protein (20 μg/lane), were from a 4 week old male mouse. BAG3 expression was detected at highest levels in skeletal (quadriceps and diaphragm) and smooth (heart) muscle specimens.

All lanes:

Western blot - Anti-Bag3 antibody (ab47124) at 1/2000 dilution

Lane 1:

Thymus lysates, total protein (20ug/lane)

Lane 2:

Spleen lysates, total protein (20ug/lane)

Lane 3:

Skeletal(quadriceps) lysates, total protein (20ug/lane)

Lane 4:

Skeletal(diaphragm) lysates, total protein (20ug/lane)

Lane 5:

Smooth (heart) muscle lysates, total protein (20ug/lane)

Predicted band size: 62 kDa

Observed band size: 86 kDa

false

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Bag3 antibody (AB47124)

ICC/IF image of ab47124 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab47124) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

• WB

Supplier Data

Western blot - Anti-Bag3 antibody (AB47124)

All lanes:

Western blot - Anti-Bag3 antibody (ab47124)

All lanes:

Hela lysate at 0.5 mg/mL

Predicted band size: 62 kDa

false