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リコンビナントRabMAb

Anti-BAFF 抗体 [EPR22238] - BSA and Azide free (ab245833)

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Immunoprecipitation - Anti-BAFF antibody [EPR22238] - BSA and Azide free (ab245833)
  • Flow Cytometry - Anti-BAFF antibody [EPR22238] - BSA and Azide free (ab245833)
  • Immunocytochemistry/ Immunofluorescence - Anti-BAFF antibody [EPR22238] - BSA and Azide free (ab245833)
  • Anti-BAFF antibody [EPR22238] - BSA and Azide free (ab245833)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22238] to BAFF - BSA and Azide free
  • Suitable for: WB, ICC/IF, IP, Flow Cyt
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Unconjugated

製品の概要

  • 製品名

    Anti-BAFF antibody [EPR22238] - BSA and Azide free
    BAFF 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR22238] to BAFF - BSA and Azide free

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, ICC/IF, IP, Flow Cytmore details
    適用なし: IHC-P

  • 種交差性

    交差種: Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • ICC/IF: U937 cells. Flow Cyt: HL-60 cells. IP: U937 whole cell lysate.

  • 特記事項

    ab245833 is the carrier-free version of ab224710.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab245833の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
Use at an assay dependent concentration. Detects a band of approximately 30, 32 kDa (predicted molecular weight: 31 kDa).
ICC/IF
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.
特記事項
WB
Use at an assay dependent concentration. Detects a band of approximately 30, 32 kDa (predicted molecular weight: 31 kDa).
ICC/IF
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.
追加情報
Is unsuitable for IHC-P.

ターゲット情報

  • 機能

    Cytokine that binds to TNFRSF13B/TACI and TNFRSF17/BCMA. TNFSF13/APRIL binds to the same 2 receptors. Together, they form a 2 ligands -2 receptors pathway involved in the stimulation of B-and T-cell function and the regulation of humoral immunity. A third B-cell specific BAFF-receptor (BAFFR/BR3) promotes the survival of mature B-cells and the B-cell response.

  • 組織特異性

    Abundantly expressed in peripheral blood Leukocytes and is specifically expressed in monocytes and macrophages. Also found in the spleen, lymph node, bone marrow, T-cells and dendritic cells. A lower expression seen in placenta, heart, lung, fetal liver, thymus, and pancreas.

  • 配列類似性

    Belongs to the tumor necrosis factor family.

  • 翻訳後修飾

    The soluble form derives from the membrane form by proteolytic processing.
    N-glycosylated.

  • 細胞内局在

    Secreted and Cell membrane.
  • Information by UniProt

  • 参照データベース

    • 別名

      • ApoL related ligand TALL 1 antibody
      • B cell Activating Factor antibody
      • B lymphocyte stimulator antibody
      • B-cell-activating factor antibody
      • BAFF antibody
      • BLyS antibody
      • CD 257 antibody
      • CD257 antibody
      • CD257 antigen antibody
      • Delta BAFF antibody
      • Dendritic cell derived TNF like molecule antibody
      • Dendritic cell-derived TNF-like molecule antibody
      • DTL antibody
      • DTL precursor antibody
      • PRO738 antibody
      • soluble form antibody
      • TALL 1 antibody
      • TALL-1 antibody
      • TALL1 antibody
      • THANK antibody
      • TN13B_HUMAN antibody
      • TNF and APOL related leukocyte expressed ligand 1 antibody
      • TNF homolog that activates apoptosis antibody
      • TNF homolog that activates apoptosis NKFB and JNK. antibody
      • TNF- and APOL-related leukocyte expressed ligand 1 antibody
      • TNFSF13B antibody
      • TNFSF20 antibody
      • TNLG7A antibody
      • Tumor necrosis factor (ligand) superfamily member 13b antibody
      • Tumor necrosis factor ligand 7A antibody
      • Tumor necrosis factor ligand superfamily member 13b antibody
      • Tumor necrosis factor ligand superfamily member 20 antibody
      • Tumor necrosis factor like protein ZTNF4 antibody
      • Tumor necrosis factor superfamily member 13B antibody
      • UNQ401 antibody
      • ZTNF4 antibody
      see all

    画像

    • Immunoprecipitation - Anti-BAFF antibody [EPR22238] - BSA and Azide free (ab245833)
      Immunoprecipitation - Anti-BAFF antibody [EPR22238] - BSA and Azide free (ab245833)

      BAFF was immunoprecipitated from 0.35 mg of U937 (human histiocytic lymphoma cell line) whole cell lysate with ab224710 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab224710 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.

      Lane 1: U937 whole cell lysate 10 µg (Input). 

      Lane 2: ab224710 IP in U937 whole cell lysate. 

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab224710 in U937 whole cell lysate.

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 20 seconds.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab224710).

    • Flow Cytometry - Anti-BAFF antibody [EPR22238] - BSA and Azide free (ab245833)
      Flow Cytometry - Anti-BAFF antibody [EPR22238] - BSA and Azide free (ab245833)

      Flow cytometric analysis of Daudi (human Burkitt's lymphoma cell line; left panel) and HL-60 (human promyelocytic leukemia cell line, right panel) cell lines labeling BAFF with ab224710 at 1/500 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.

      Negative control: Daudi. (PMID: 20951740)

      Gated on viable cells.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab224710).

    • Immunocytochemistry/ Immunofluorescence - Anti-BAFF antibody [EPR22238] - BSA and Azide free (ab245833)
      Immunocytochemistry/ Immunofluorescence - Anti-BAFF antibody [EPR22238] - BSA and Azide free (ab245833)

      Immunofluorescent analysis of 100% methanol-fixed U937 (human histiocytic lymphoma cell line) cells labeling BAFF with ab224710 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on U937 cells.

      The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

      Confocal image showing membranous staining in U-937 cells.

      We don’t recommend PFA fixation, since the result shows some background staining in the nuclear.

      Negative control: HEK-293 PMID: 17393395.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab224710).

    • Anti-BAFF antibody [EPR22238] - BSA and Azide free (ab245833)
      Anti-BAFF antibody [EPR22238] - BSA and Azide free (ab245833)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab245833 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab245833 は論文での使用が確認できていません。

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