Anti-BAFF 抗体 (ab8396)
Key features and details
- Rabbit polyclonal to BAFF
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-BAFF antibody
BAFF 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to BAFF -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, WBmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide, corresponding to amino acids 254-269 of Human BAFF.
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ポジティブ・コントロール
- HL60 cell lysate
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 7.2
Preservative: 0.02% Sodium azide -
Concentration information loading...
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精製度
Ion Exchange Chromatography -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
軽鎖の種類
unknown -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab8396の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
Use a concentration of 1 µg/ml.
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WB |
Use a concentration of 0.25 - 1 µg/ml. Detects a band of approximately 34 kDa.
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特記事項 |
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ICC/IF
Use a concentration of 1 µg/ml. |
WB
Use a concentration of 0.25 - 1 µg/ml. Detects a band of approximately 34 kDa. |
ターゲット情報
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機能
Cytokine that binds to TNFRSF13B/TACI and TNFRSF17/BCMA. TNFSF13/APRIL binds to the same 2 receptors. Together, they form a 2 ligands -2 receptors pathway involved in the stimulation of B-and T-cell function and the regulation of humoral immunity. A third B-cell specific BAFF-receptor (BAFFR/BR3) promotes the survival of mature B-cells and the B-cell response. -
組織特異性
Abundantly expressed in peripheral blood Leukocytes and is specifically expressed in monocytes and macrophages. Also found in the spleen, lymph node, bone marrow, T-cells and dendritic cells. A lower expression seen in placenta, heart, lung, fetal liver, thymus, and pancreas. -
配列類似性
Belongs to the tumor necrosis factor family. -
翻訳後修飾
The soluble form derives from the membrane form by proteolytic processing.
N-glycosylated. -
細胞内局在
Secreted and Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 10673 Human
- Entrez Gene: 24099 Mouse
- Entrez Gene: 498666 Rat
- Omim: 603969 Human
- SwissProt: Q9Y275 Human
- SwissProt: Q9WU72 Mouse
- Unigene: 525157 Human
- Unigene: 596110 Human
see all -
別名
- ApoL related ligand TALL 1 antibody
- B cell Activating Factor antibody
- B lymphocyte stimulator antibody
see all
画像
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All lanes : Anti-BAFF antibody (ab8396) at 1 µg/ml
Lane 1 : Human HL60 cell lysate
Lane 2 : Murine spleen tissue lysate
Observed band size: 34 kDa why is the actual band size different from the predicted? -
ab8396 at 1µg/ml staining BAFF in HL60 cells by ICC/IF
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab8396 は 3 報の論文で使用されています。
- Cao Y et al. BAFF is involved in the pathogenesis of IgA nephropathy by activating the TRAF6/NF-?B signaling pathway in glomerular mesangial cells. Mol Med Rep 21:795-805 (2020). PubMed: 31974601
- Walls A et al. Characterization of B-Cells in tonsils of patients diagnosed with pediatric autoimmune neuropsychiatric disorder associated streptococcus. Int J Pediatr Otorhinolaryngol 80:49-52 (2016). WB ; Human . PubMed: 26746612
- Panchanathan R & Choubey D Murine BAFF expression is up-regulated by estrogen and interferons: implications for sex bias in the development of autoimmunity. Mol Immunol 53:15-23 (2013). PubMed: 22784990