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Anti-Bad (phospho S99) 抗体 (ab28824)

Reviews (1)Q&A (2)References (16)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bad (phospho S99) antibody (ab28824)
  • Western blot - Anti-Bad (phospho S99) antibody (ab28824)

Key features and details

  • Rabbit polyclonal to Bad (phospho S99)
  • Suitable for: IHC-P, WB
  • Reacts with: Human
  • Isotype: IgG

製品の概要

  • 製品名

    Anti-Bad (phospho S99) antibody
    Bad 一次抗体 製品一覧

  • 製品の詳細

    Rabbit polyclonal to Bad (phospho S99)

  • 由来種

    Rabbit

  • 特異性

    Predicited to react with Mouse S136 and Rat S137

  • アプリケーション

    適用あり: IHC-P, WBmore details

  • 種交差性

    交差種: Human
    交差が予測される動物種: Mouse, Rat

  • 免疫原

    Synthetic peptide within Human Bad (phospho S99). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
    Database link: Q92934

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab28824の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-P
Use at an assay dependent concentration.
WB
1/500 - 1/1000. Predicted molecular weight: 19 kDa.
特記事項
IHC-P
Use at an assay dependent concentration.
WB
1/500 - 1/1000. Predicted molecular weight: 19 kDa.

ターゲット情報

  • 機能

    Promotes cell death. Successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. Can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2 (By similarity). Appears to act as a link between growth factor receptor signaling and the apoptotic pathways.

  • 組織特異性

    Expressed in a wide variety of tissues.

  • 配列類似性

    Belongs to the Bcl-2 family.

  • ドメイン

    Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.

  • 翻訳後修飾

    Phosphorylated on one or more of Ser-75, Ser-99, Ser-118 and Ser-134 in response to survival stimuli, which blocks its pro-apoptotic activity. Phosphorylation on Ser-99 or Ser-75 promotes heterodimerization with 14-3-3 proteins. This interaction then facilitates the phosphorylation at Ser-118, a site within the BH3 motif, leading to the release of Bcl-X(L) and the promotion of cell survival. Ser-99 is the major site of AKT/PKB phosphorylation, Ser-118 the major site of protein kinase A (CAPK) phosphorylation. Ser-75 is phosphorylated by AKT/PKB, protein kinase A and PIM2.

  • 細胞内局在

    Mitochondrion outer membrane. Cytoplasm. Upon phosphorylation, locates to the cytoplasm.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • AI325008 antibody
    • BAD antibody
    • BAD_HUMAN antibody
    • BBC 2 antibody
    • BBC2 antibody
    • BBC6 antibody
    • Bcl 2 Antagonist of Cell Death antibody
    • Bcl 2 Binding Component 6 antibody
    • BCL X / BCL 2 Binding Protein antibody
    • BCL X Binding Protein antibody
    • Bcl XL/Bcl 2 Associated Death Promoter antibody
    • Bcl-2-binding component 6 antibody
    • Bcl-2-like protein 8 antibody
    • Bcl-XL/Bcl-2-associated death promoter antibody
    • Bcl2 antagonist of cell death antibody
    • BCL2 antagonist of cell death protein antibody
    • BCL2 associated agonist of cell death antibody
    • Bcl2 Associated Death Promoter antibody
    • BCL2 binding component 6 antibody
    • BCL2 binding protein antibody
    • Bcl2 Like 8 Protein antibody
    • Bcl2-L-8 antibody
    • BCL2L8 antibody
    • Proapoptotic BH3 Only Protein antibody
    see all

画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bad (phospho S99) antibody (ab28824)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bad (phospho S99) antibody (ab28824)
    Immunohistochemical analysis of paraffin embedded breast carcinoma, using ab28824. Left: Untreated. Right: Treated with synthesized phosphopeptide.
  • Western blot - Anti-Bad (phospho S99) antibody (ab28824)
    Western blot - Anti-Bad (phospho S99) antibody (ab28824)
    All lanes : Anti-Bad (phospho S99) antibody (ab28824) at 1/500 dilution

    Lane 1 : MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell Lysate
    Lane 2 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Predicted band size: 19 kDa
    Observed band size: 26 kDa why is the actual band size different from the predicted?

参考文献 (16)

ab28824 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab28824 は 16 報の論文で使用されています。

  • Chen K  et al. Endogenous Cyclin D1 Promotes the Rate of Onset and Magnitude of Mitogenic Signaling via Akt1 Ser473 Phosphorylation. Cell Rep 42:112595 (2023). PubMed: 37224013
  • Narla ST  et al. AKT Signaling Downstream of KGF Is Necessary and Sufficient for Blocking Cyclophosphamide Bladder Injury. Am J Pathol 192:604-612 (2022). PubMed: 35063403
  • Narla ST  et al. FGF7 peptide (FGF7p) mimetic mitigates bladder urothelial injury from cyclophosphamide. Physiol Rep 10:e15241 (2022). PubMed: 35388988
  • Zhang B  et al. The Dual Dose-Dependent Effects of Corticosterone on Hippocampal Cell Apoptosis After Traumatic Brain Injury Depend on the Activation Ratio of Mineralocorticoid Receptors to Glucocorticoid Receptors. Front Pharmacol 12:713715 (2021). PubMed: 34381366
  • Chen K  et al. Endogenous Cyclin D1 Promotes the Rate of Onset and Magnitude of Mitogenic Signaling via Akt1 Ser473 Phosphorylation. Cell Rep 32:108151 (2020). PubMed: 32937140
View all Publications for this product

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1-3 of 3 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-Bad (phospho S136) antibody

 Good
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Oral squamous cell carcinoma)
Permeabilization
Yes - PBS containing 0,25% Triton X-100
Specification
Oral squamous cell carcinoma
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 24°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

87andressaduarte@gmail.com

Verified customer

投稿 Sep 29 2015

Question

Dear Sr/ Madam, I am a senior scientist working on signal transduction areas. Previously I have published several papers using BAD, phospho Ser112 and phosphoSer136 antibodies that were obtained from Cell Signaling Technology. This time my friend suggested me to use the above antibodies from abcam. I wonder if can send us a free sample to check the consistacne of these abs in our hands. If they find suitable, we repeatedly order these atnibodies alongwith several other antibodies such as Akt, pAkt, ERK, pERK, etc. thanks

Read More
Answer

Thank you for your enquiry and interest in our products.

I am sorry to confirm that because we carry over 70,000 products, it is regrettably not feasible for us to keep small sample sizes to send to customers.

We are happy to reassure our customers that all of our products are covered by our Abpromise, which guarantees that the product will work in the applications and species specified on the datasheet, or we will offer a replacement, credit, or refund within 6 months of purchase.

If the product is to be used in an untested species or application, you may be eligible for our testing discount program if the antibody has not yet been purchased. Please do not hesitate to contact me again by replying to this email prior to purchase and I will be pleased to send you more information.

With regards to which antibodies you would like to use, do you already have product catalog code numbers? If you would like some assistance to find suitable antibodies, I would appreciate if you are able to provide information on the application and species you will be testing. I can then find some suitably tested antibodies covered by our 6 month guarantee for your experiments.

I hope this information is helpful. I look forward to hearing from you with the requested information.

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Question

Thanks for take a look over this inquiry. attached please find our answers to the questions you asked.  I hope it to be of assistance for you the provide us with good and helpful support. I look forward to your reply soon, and a possible replacement for this item expected. Best regard

Read More
Answer

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement (ab1893) with the order number XXXXXXX. To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research.  

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