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AB245549

Anti-BACH1.3 抗体

Anti-BACH1.3 antibody

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(1 Publication)

Rabbit Polyclonal BACH1.3 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human Transcription regulator protein BACH1 aa 650-700.

別名を表示する

Transcription regulator protein BACH1, BTB and CNC homolog 1, HA2303, BACH1

2 Images
Immunoprecipitation - Anti-BACH1.3 antibody (AB245549)
  • IP

Supplier Data

Immunoprecipitation - Anti-BACH1.3 antibody (AB245549)

BACH1.3 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab245549 at 6 μg/mg lysate. Western blot was performed from the immunoprecipitate using ab245549 at 1 μg/ml.

Lane 1 : ab245549 IP in HeLa whole cell lysate.
Lane 2 : Control IgG IP in HeLa whole cell lysate.

Detection : Chemiluminescence with exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-BACH1.3 antibody (ab245549)

Predicted band size: 82 kDa

false

Western blot - Anti-BACH1.3 antibody (AB245549)
  • WB

Supplier Data

Western blot - Anti-BACH1.3 antibody (AB245549)

All lanes:

Western blot - Anti-BACH1.3 antibody (ab245549) at 0.1 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Lane 4:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Predicted band size: 82 kDa

true

Exposure time: 3min

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

No

交差種

Human

アプリケーション

WB, IP

applications

免疫原

Synthetic Peptide within Human Transcription regulator protein BACH1 aa 650-700. The exact immunogen used to generate this antibody is proprietary information.

O14867

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" } } }
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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Immunogen
精製に関する特記事項
ab245549 was affinity purified using an epitope specific to BACH1.3 immobilized on solid support.
バッファー組成
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

The BACH1.3 gene encodes a transcription regulator protein also known as BTB and CNC homology 1 basic leucine zipper transcription factor 1. The protein weighs approximately 105 kDa and you can find it in various tissues including the liver lungs and kidneys. It binds to the antioxidant response elements (ARE) in DNA regulating the transcription of genes involved in oxidative stress response. BACH1.3 functions by repressing gene activation through heterodimerization with small Maf proteins which helps maintain cellular redox balance.
Biological function summary

BACH1.3 modulates gene expression by forming complexes with small Maf proteins which are necessary for its regulatory activity. This protein plays a role in cellular oxidative stress management and heme metabolism. Through its function in stress response regulation BACH1.3 influences the expression of several genes such as heme oxygenase-1 (HO-1) important for detoxifying reactive oxygen species. By controlling these pathways BACH1.3 supports cell protection and adaptation to environmental oxidative stress.

Pathways

BACH1.3 participates in the oxidative stress response and heme degradation pathways. It partners with the KEAP1-NRF2 pathway where it acts as a negative regulator of NRF2 target genes by competing with NRF2 for binding to ARE sequences. This action impacts the transcription of antioxidant genes. BACH1.3 indirectly affects the expression of proteins like HO-1 and NAD(P)H:quinone oxidoreductase 1 (NQO1) moderating their protective effects in the cell.

Altered BACH1.3 function associates with cancer and cardiovascular diseases. Its involvement in improper oxidative stress response can lead to tumor progression and resistance to therapeutic agents in some cancers. Additionally in cardiovascular diseases BACH1.3 connects to abnormal arterial remodeling due to its regulation of genes involved in oxidative stress. This target interacts with proteins like KEAP1 and NRF2 modifying disease outcomes through changes in redox-sensitive signaling and gene expression.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Transcriptional regulator that acts as a repressor or activator, depending on the context. Binds to NF-E2 DNA binding sites. Plays important roles in coordinating transcription activation and repression by MAFK (By similarity). Together with MAF, represses the transcription of genes under the control of the NFE2L2 oxidative stress pathway (PubMed : 24035498).
See full target information Transcription regulator protein BACH1
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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Cellular & molecular immunology 19:516-526 PubMed34983952

2022

Enhancing the HSV-1-mediated antitumor immune response by suppressing Bach1.

Applications

Unspecified application

Species

Unspecified reactive species

Chaohu Pan,Qiaomei Cai,Xiaorong Li,Lili Li,Liping Yang,Yu Chen,Junxiao Liu,Wancheng Liu,Meiling Gao,Tianqi Sui,Xiaoyang Wang,Huiming Fan,Jiayin Ruan,Yueyue Shi,Saihua Chen,Lucy S Cheng,Jiayong Liu,Heng Yang,Genhong Cheng
View all publications
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代替製品

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