Anti-BACE1 抗体 (ab2077)

Thank you for contacting us.

Your credit note ID is xxxxx.

I am sorry that this antibody did not perform as stated on the datasheet, I have asked our Finance department to issue a credit note for you. The credit note may be used in one of the following ways:

(1) Redeemed against the original invoice if this hasn't already been paid.
(2) Held on the account for use against a future order.
(3) A full refund can be offered where no other invoices are outstanding.

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I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service should you require further expert advice

Thank you for your message and for providing this further information.

I am sorry to confirm that we can not make any further suggestions in this case to improve the results. It seems that you regrettably received a bad vial. For that reason I would be pleased to arrange a refund, credit note or free of charge replacement in compensation.

I look forward to hearing from you with details of how you would like to proceed.

Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from our Anti-BACE1 antibody (ab2077) .

The details you have kindly provided will enable us to investigate this case for you and this is also helpful in our records for monitoring of quality.

I would like to reassure you that ab2077 is tested and covered by our 6 month guarantee for use in WB and mouse as well as human samples. In the event that a product is not functioning in the applications cited on the product data sheet, we will be pleased to provide a credit note or free of charge replacement.

Regrettably reviewing all the details there are not many suggestions we can make to improve the protocol. However, I would like to investigate the case a bit further for you. Before deciding how to proceed with this particular case, I would appreciate if you can confirm some details of the procedure.

1. Could you please confirm if a loading control was included and what the results were? A loading control assures that the samples used are of good quality.

2. We have some data that suggest that the BACE1 protein is difficult to transfer to the membrane in WB application. I would appreciate if you could confirm if the transfer of protein onto the membrane was assased and what was the result?

3. Could you confirm the isotype that is recognised by the secondary antibody and weather it is known to work well with other primary antibodies?

Thank you very much for your cooperation. Please do not hesitate to contact me again with the further requested details. I look forward to your answer and am sure we will be able to find a solution.

Thank you for your reply.

I am sending you a new vial of ab2077, your new order number is ** and you should have the new antibody by 10.30am tomorrow morning. If there is anything else I can help you with, or if you have any issues with the new vial of the antibody please let me know.

I hope your experiments go well.

Thank you for your reply.

If sending you a new aliquot of the cell lysate would help you to complete your experiments, then I would be happy to do so. However, I am confused as to why you think that the current vial of ab2077 that you have is ok when it is not giving the expected signal. I was hoping that if I send you a different antibody against the same target, then you would be able to detect your band of interest.

As I mentioned previously, I would be happy to send a new vial of cell lysate, I just wanted to confirm with you the course of action that you wish to take and to double check that you did not want to try a different primary antibody.

I look forward to your reply.

Thank you for your reply.

I am sorry about the issues you have been having with ab2077 in western blot. I cannot send you all of the reagents that you are requesting, I can either send 1 free antibody as a replacement for ab2077, or I can process a refund for the cost of the original purchase.

Please let me know how you would like to proceed.

Thank you for calling Abcam yesterday.

I am sorry about the issues you have been having with ab2077 in western blot. Having talked to my colleagues, I think the best way to move forward with this is to either replace the antibody that you currently have or to refund the cost of the original purchase.

I do not think that there are any protocol tips that I could give that would help resolve the banding pattern that you are seeing and can only assume that the vial of ab2077 that you received is not working correctly.

A few alternative BACE1 antibodies to consider are:

https://www.abcam.com/BACE1-antibody-EPR3956-ab108394.html
https://www.abcam.com/BACE1-antibody-ab63954.html



If either of these antibodies would work for you (or if there is another BACE1 antibody that you would like to try), please let me know and I will be happy to send it out to you.

I look forward to your reply.

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued the free of charge blocking peptide for this product with the order number I have also included our protocol for blocking with the immunizing peptide for your review. To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research.