Anti-BACE1 抗体 (ab10716)
Key features and details
- Rabbit polyclonal to BACE1
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-BACE1 antibody
BACE1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to BACE1 -
由来種
Rabbit -
特異性
Detects transfected beta-secretase 1. -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Guinea pig, Cow
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免疫原
Synthetic peptide corresponding to Human BACE1 aa 485-501 (C terminal).
Sequence:CLRQQHDDFADDISLLK
(Peptide available asab7883) -
ポジティブ･コントロール
- WB: U87-MG, HeLa and Mouse brain cell lysate. ICC/IF: SH-SY5Y cells, Neuro-2a cells, HeLa cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.1% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab10716の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000. Predicted molecular weight: 56 kDa.
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| ICC/IF |
1/20 - 1/200.
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| 特記事項 |
|---|
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WB
1/1000. Predicted molecular weight: 56 kDa. |
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ICC/IF
1/20 - 1/200. |
ターゲット情報
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機能
Responsible for the proteolytic processing of the amyloid precursor protein (APP). Cleaves at the N-terminus of the A-beta peptide sequence, between residues 671 and 672 of APP, leads to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase. -
組織特異性
Expressed at high levels in the brain and pancreas. In the brain, expression is highest in the substantia nigra, locus coruleus and medulla oblongata. -
配列類似性
Belongs to the peptidase A1 family. -
ドメイン
The transmembrane domain is necessary for its activity. It determines its late Golgi localization and access to its substrate, APP. -
翻訳後修飾
Glycosylated. -
細胞内局在
Membrane. Golgi apparatus > trans-Golgi network. Endoplasmic reticulum. Endosome. Cell surface. Predominantly localized to the later Golgi/trans-Golgi network (TGN) and minimally detectable in the early Golgi compartments. A small portion is also found in the endoplasmic reticulum, endosomes and on the cell surface. - Information by UniProt
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参照データベース
- Entrez Gene: 614333 Cow
- Entrez Gene: 23621 Human
- Entrez Gene: 23821 Mouse
- Omim: 604252 Human
- SwissProt: Q2HJ40 Cow
- SwissProt: P56817 Human
- SwissProt: P56818 Mouse
- Unigene: 504003 Human
see all -
別名
- APP beta secretase antibody
- Asp 2 antibody
- ASP2 antibody
see all
画像
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Western blot - Anti-BACE1 antibody (ab10716)All lanes : Anti-BACE1 antibody (ab10716) at 1/1000 dilution
Lane 1 : U87-MG cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Mouse brain cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : HRP-conjugated secondary antibody
Predicted band size: 56 kDa
Observed band size: 42,56,70 kDa why is the actual band size different from the predicted?Western blot analysis of BACE1 was performed by loading Samples onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked at 4°C overnight. The membrane was probed with primary antibody overnight at 4°C, washed in TBST, and probed with secondary antibody for 1 hour at room temperature in the dark. Chemiluminescent detection was performed using Pierce ECL Plus Western Blotting Substrate.
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Immunocytochemistry/ Immunofluorescence - Anti-BACE1 antibody (ab10716)Immunocytochemistry/Immunofluorescent analysis of BACE1 (green) showing staining in the Golgi apparatus of SH-SY5Y cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a10716 in 3% BSA-PBS at a dilution of 1/100 and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
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Immunocytochemistry/ Immunofluorescence - Anti-BACE1 antibody (ab10716)Immunocytochemistry/Immunofluorescent analysis of BACE1 (green) showing staining in the Golgi apparatus of Neuro-2a cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with ab10716 in 3% BSA-PBS at a dilution of 1/100 and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
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Immunocytochemistry/ Immunofluorescence - Anti-BACE1 antibody (ab10716)Immunocytochemistry/Immunofluorescent analysis of BACE1 (green) showing staining in the Golgi apparatus of HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with ab10716 in 3% BSA-PBS at a dilution of 1/100 and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (7)
ab10716 は 7 報の論文で使用されています。
- Huang Y et al. Cellular cholesterol loss by DHCR24 knockdown leads to Aβ production by changing APP intracellular localization. J Lipid Res 64:100367 (2023). PubMed: 37011864
- Seki T et al. Galectin 3-binding protein suppresses amyloid-ß production by modulating ß-cleavage of amyloid precursor protein. J Biol Chem 295:3678-3691 (2020). PubMed: 31996371
- Quan Q et al. Ginsenoside Rg1 reduces β-amyloid levels by inhibiting CDΚ5-induced PPARγ phosphorylation in a neuron model of Alzheimer's disease. Mol Med Rep 22:3277-3288 (2020). PubMed: 32945455
- Al-Atrache Z et al. Astrocytes infected with Chlamydia pneumoniae demonstrate altered expression and activity of secretases involved in the generation of ß-amyloid found in Alzheimer disease. BMC Neurosci 20:6 (2019). PubMed: 30786875
- You M et al. Royal Jelly Alleviates Cognitive Deficits and ß-Amyloid Accumulation in APP/PS1 Mouse Model Via Activation of the cAMP/PKA/CREB/BDNF Pathway and Inhibition of Neuronal Apoptosis. Front Aging Neurosci 10:428 (2018). PubMed: 30687079
- Frontiñán-Rubio J et al. Sex-dependent co-occurrence of hypoxia and ß-amyloid plaques in hippocampus and entorhinal cortex is reversed by long-term treatment with ubiquinol and ascorbic acid in the 3?×?Tg-AD mouse model of Alzheimer's disease. Mol Cell Neurosci 92:67-81 (2018). IHC-Fr ; Mouse . PubMed: 29953929
- Schnöder L et al. Deficiency of Neuronal p38a MAPK Attenuates Amyloid Pathology in Alzheimer Disease Mouse and Cell Models through Facilitating Lysosomal Degradation of BACE1. J Biol Chem 291:2067-79 (2016). PubMed: 26663083