Anti-B4GALT3 抗体 (ab74825)
Key features and details
- Rabbit polyclonal to B4GALT3
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-B4GALT3 antibody
B4GALT3 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to B4GALT3 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat
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免疫原
Synthesized peptide derived from an internal region of human B4GALT3
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ポジティブ･コントロール
- WB: extracts from HepG2 cells, HeLa cells and Jurkat cells IHC-P: human liver carcinoma tissue
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab74825の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/1000. Predicted molecular weight: 44 kDa.
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| IHC-P |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
|
WB
1/500 - 1/1000. Predicted molecular weight: 44 kDa. |
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IHC-P
Use at an assay dependent concentration. |
ターゲット情報
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機能
Responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids. -
組織特異性
Found in various tissues. Highest expression in placenta, prostate, testis, ovary, intestine and muscle, and in fetal brain. -
パスウェイ
Protein modification; protein glycosylation. -
配列類似性
Belongs to the glycosyltransferase 7 family. -
細胞内局在
Golgi apparatus > Golgi stack membrane. Trans cisternae of Golgi stack. - Information by UniProt
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参照データベース
- Entrez Gene: 8702 Human
- Entrez Gene: 8703 Human
- Entrez Gene: 8704 Human
- Entrez Gene: 53418 Mouse
- Entrez Gene: 56375 Mouse
- Entrez Gene: 57370 Mouse
- Entrez Gene: 303923 Rat
- Entrez Gene: 494342 Rat
see all -
別名
- 4-galactosyltransferase 3 antibody
- 4-galactosyltransferase antibody
- 4-GalTase 3 antibody
see all
画像
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Western blot - Anti-B4GALT3 antibody (ab74825)All lanes : Anti-B4GALT3 antibody (ab74825) at 1/500 dilution
Lane 1 : extracts from HepG2 cells
Lane 2 : extracts from HeLa cells
Lane 3 : extracts from Jurkat cells
Lane 4 : extracts from Jurkat cells with immunizing peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 44 kDa
Observed band size: 44 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-B4GALT3 antibody (ab74825)ab74825, at a dilution of 1/50, staining B4GALT3 in paraffin embedded human liver carcinoma tissue sections by Immunohistochemistry in the absence (left image) or presence (right image) of the immunizing peptide.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab74825 は 1 報の論文で使用されています。
- Zhou H et al. B4GALT family mediates the multidrug resistance of human leukemia cells by regulating the hedgehog pathway and the expression of p-glycoprotein and multidrug resistance-associated protein 1. Cell Death Dis 4:e654 (2013). WB ; Human . PubMed: 23744354