AB74825

Anti-B4GALT3 抗体

Name: Anti-B4GALT3 抗体 (ab74825) | Abcam
Brand: Abcam Limited
SKU: AB74825
Price: 74000 JPY
Availability: InStock

Anti-B4GALT3 antibody

Be the first to review this product! Submit a review

(1 Publication)

Rabbit Polyclonal B4GALT3 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human Beta-1,4-galactosyltransferase 3.

別名を表示する

Beta4Gal-T3, b4Gal-T3, N-acetyllactosamine synthase, Nal synthase, B4GALT3

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-B4GALT3 antibody (AB74825)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-B4GALT3 antibody (AB74825)

ab74825, at a dilution of 1/50, staining B4GALT3 in paraffin embedded human liver carcinoma tissue sections by Immunohistochemistry in the absence (left image) or presence (right image) of the immunizing peptide.

Unknown

Western blot - Anti-B4GALT3 antibody (AB74825)

All lanes:

Western blot - Anti-B4GALT3 antibody (ab74825) at 1/500 dilution

Lane 1:

extracts from HepG2 cells at 30 µg

Lane 2:

extracts from HeLa cells at 30 µg

Lane 3:

extracts from Jurkat cells at 30 µg

Lane 4:

extracts from Jurkat cells at 30 µg with immunizing peptide

Predicted band size: 44 kDa

Observed band size: 44 kDa

false

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

WB, IHC-P

applications

免疫原

Synthetic Peptide within Human Beta-1,4-galactosyltransferase 3. The exact immunogen used to generate this antibody is proprietary information.

O60512

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

style

left-column

style

left-column

出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

精製に関する特記事項

The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.

バッファー組成

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

style

left-column

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

B4GALT3 also known as Beta-14-galactosyltransferase 3 is an enzyme that plays an important role in transferring galactose from UDP-galactose to N-acetylglucosamine on polypeptides forming N-linked oligosaccharides. The enzyme is about 43 kDa in mass. It is mainly expressed in the Golgi apparatus of cells influencing the biosynthesis of glycoconjugates. B4GALT3 activity is evident across various tissues showing a broad expression pattern.

Biological function summary

B4GALT3 contributes significantly to the biosynthesis and modification of glycoproteins and glycolipids. It is a part of the glycosyltransferase family which functions in forming glycosidic bonds. This enzyme does not work alone; it often associates with other enzymes within the Golgi complex supporting diverse cellular processes like cell adhesion and receptor-ligand interactions by creating complex glycans.

Pathways

B4GALT3 is involved in the N-glycan biosynthesis and glycosaminoglycan biosynthesis pathways. In these pathways B4GALT3 interacts with proteins such as GlcNAcT glycosyltransferases aiding in the structural diversification of glycoconjugates. These pathways are essential for proper cell surface receptor function and signaling linking B4GALT3 to processes necessary for normal cell communication and structural integrity.

Alterations in B4GALT3 activity have associations with congenital disorders of glycosylation and certain cancers. Changes in glycosylation patterns by B4GALT3 can influence tumor progression and metastasis by impacting cellular recognition and adhesion. The enzyme also interacts with proteins like α3β1 integrin in cancer influencing cell movement and survival. Understanding these links could help target B4GALT3 in therapeutic strategies for these conditions.

style

left-column

style

left-column

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

ターゲットの情報

Responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids.

See full target information Beta-1,4-galactosyltransferase 3

style

left-column

文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 4:e654 PubMed23744354

2013

B4GALT family mediates the multidrug resistance of human leukemia cells by regulating the hedgehog pathway and the expression of p-glycoprotein and multidrug resistance-associated protein 1.

Applications

Species

Human

H Zhou,H Ma,W Wei,D Ji,X Song,J Sun,J Zhang,L Jia

View all publications

style

left-column

style

left-column

style

right-column

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-B4GALT3 抗体

Anti-B4GALT3 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-B4GALT3 antibody (AB74825)

Western blot - Anti-B4GALT3 antibody (AB74825)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

精製に関する特記事項

バッファー組成

出荷温度

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (1)

組合せ製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise