Anti-B3GALT4 抗体 [EPR11650] (ab169759)
![Western blot - Anti-B3GALT4 antibody [EPR11650] (ab169759)](https://www.abcam.co.jp/ps/products/169/ab169759/Images/ab169759-168580-anti-b3galt4-antibody-epr11650-western-blot.jpg "Western blot - Anti-B3GALT4 antibody [EPR11650] (ab169759)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11650] to B3GALT4
- Suitable for: Flow Cyt (Intra), IHC-P, WB
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-B3GALT4 antibody [EPR11650]
B3GALT4 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR11650] to B3GALT4 -
由来種
Rabbit -
アプリケーション
適用あり: Flow Cyt (Intra), IHC-P, WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat
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免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- Fetal heart, PC-3, Jurkat and U87-MG lysates ; Human breast carcinoma and Human muscle tissues ; Jurkat cells..
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR11650 -
アイソタイプ
IgG
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab169759の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt (Intra) |
1/10.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
| IHC-P |
1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
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| WB |
1/1000. Predicted molecular weight: 42 kDa.
|
| 特記事項 |
|---|
|
Flow Cyt (Intra)
1/10. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
IHC-P
1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
WB
1/1000. Predicted molecular weight: 42 kDa. |
ターゲット情報
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機能
Involved in GM1/GD1B/GA1 ganglioside biosynthesis. -
組織特異性
Highly expressed in heart, skeletal muscle and pancreas and, to a lesser extent, in brain, placenta, kidney, liver and lung. -
パスウェイ
Protein modification; protein glycosylation. -
配列類似性
Belongs to the glycosyltransferase 31 family. -
細胞内局在
Golgi apparatus membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 8705 Human
- Entrez Gene: 54218 Mouse
- Entrez Gene: 171079 Rat
- Omim: 603095 Human
- SwissProt: O96024 Human
- SwissProt: Q9Z0F0 Mouse
- SwissProt: O88178 Rat
- Unigene: 534375 Human
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別名
- 3-galactosyltransferase 4 antibody
- 3-galactosyltransferase antibody
- 3-GalTase 4 antibody
see all
画像
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Western blot - Anti-B3GALT4 antibody [EPR11650] (ab169759)All lanes : Anti-B3GALT4 antibody [EPR11650] (ab169759) at 1/10000 dilution
Lane 1 : Fetal tissue lysate
Lane 2 : PC-3 cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : U87-MG cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 42 kDa -
![Flow Cytometry (Intracellular) - Anti-B3GALT4 antibody [EPR11650] (ab169759)](https://www.abcam.co.jp/ps/products/169/ab169759/Images/ab169759-168579-anti-b3galt4-antibody-epr11650-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-B3GALT4 antibody [EPR11650] (ab169759)Intracellular flow cytometric analysis of permeabilized Jurkat cells labeling B3GALT4 with ab169759 at 1/10 dilution (red) or a rabbit IgG (negative) (green).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-B3GALT4 antibody [EPR11650] (ab169759)](https://www.abcam.co.jp/ps/products/169/ab169759/Images/ab169759-168578-anti-b3galt4-antibody-epr11650-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-B3GALT4 antibody [EPR11650] (ab169759)Immunohistochemical analysis of paraffin-embedded Human muscle tissue labeling B3GALT4 with ab169759 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-B3GALT4 antibody [EPR11650] (ab169759)](https://www.abcam.co.jp/ps/products/169/ab169759/Images/ab169759-168577-anti-b3galt4-antibody-epr11650-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-B3GALT4 antibody [EPR11650] (ab169759)Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling B3GALT4 with ab169759 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Anti-B3GALT4 antibody [EPR11650] (ab169759)](https://www.abcam.co.jp/ps/products/169/ab169759/Images/ab169759-5-benefits-of-recombinant-antibodies.png)
Anti-B3GALT4 antibody [EPR11650] (ab169759)
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab169759 は論文での使用が確認できていません。