Anti-B1R 抗体 (ab75148)
Key features and details
- Rabbit polyclonal to B1R
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-B1R antibody
B1R 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to B1R -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to Human B1R (internal sequence).
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ポジティブ･コントロール
- Extracts from K562 cells. LOVO cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab75148の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (1) |
1/500 - 1/1000. Predicted molecular weight: 40 kDa.
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| ICC/IF |
1/500 - 1/1000.
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| 特記事項 |
|---|
|
WB
1/500 - 1/1000. Predicted molecular weight: 40 kDa. |
|
ICC/IF
1/500 - 1/1000. |
ターゲット情報
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機能
This is a receptor for bradykinin. Could be a factor in chronic pain and inflammation. -
配列類似性
Belongs to the G-protein coupled receptor 1 family. Bradykinin receptor subfamily. BDKRB1 sub-subfamily. -
細胞内局在
Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 623 Human
- Omim: 600337 Human
- SwissProt: P46663 Human
- Unigene: 525572 Human
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別名
- B1 bradykinin receptor antibody
- B1BKR antibody
- B1R antibody
see all
画像
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Western blot - Anti-B1R antibody (ab75148)All lanes : Anti-B1R antibody (ab75148) at 1/500 dilution
Lane 1 : Extracts from K562 cells
Lane 2 : Extracts from K562 cells with immunising peptide at 5 µg
Lysates/proteins at 25 µg per lane.
Predicted band size: 40 kDa
Observed band size: 40 kDa
Additional bands at: 48 kDa, 50 kDa, 82 kDa. We are unsure as to the identity of these extra bands. -
Immunocytochemistry/ Immunofluorescence - Anti-B1R antibody (ab75148)Immunofluorescence analysis of B1R in LOVO cells using ab75148, at 1/500 dilution, with (right panel) or without (left panel) preincubation with the immunizing peptide.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab75148 は 2 報の論文で使用されています。
- Meng X et al. Involvement of bradykinin and bradykinin B1 receptor in patients with endometriosis. Exp Ther Med 22:1240 (2021). PubMed: 34539836
- Ghebrehiwet B et al. Soluble gC1qR is an autocrine signal that induces B1R expression on endothelial cells. J Immunol 192:377-84 (2014). WB . PubMed: 24319267