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AB61000

Anti-B MyB (phospho S577 + S581) 抗体

Anti-B MyB (phospho S577 + S581) antibody

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(2 Publications)

Rabbit Polyclonal B MyB phospho S577 + S581 antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human MYBL2 pS577 + S581.

別名を表示する

BMYB, MYBL2, Myb-related protein B, B-Myb, Myb-like protein 2

1 Images
Western blot - Anti-B MyB (phospho S577 + S581) antibody (AB61000)
  • WB

Unknown

Western blot - Anti-B MyB (phospho S577 + S581) antibody (AB61000)

All lanes:

Western blot - Anti-B MyB (phospho S577 + S581) antibody (ab61000) at 1/500 dilution

Lane 1:

extracts from K562 cells

Lane 2:

extracts from K562 cells with immunizing phosphopeptide

Predicted band size: 79 kDa

Observed band size: 79 kDa

false

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

No

交差種

Human

アプリケーション

WB

applications

免疫原

Synthetic Peptide within Human MYBL2 pS577 + S581. The exact immunogen used to generate this antibody is proprietary information.

P10244

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代替製品

Primary Antibodies

AB114055

Anti-B MyB antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-B MyB antibody (AB114055)

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  • 0
Primary Antibodies

AB12296

Anti-B MyB antibody

Western blot - Anti-B MyB antibody (AB12296)

  • 5
  • 1
Primary Antibodies

AB232521

Anti-B MyB (phospho T487) antibody [EPR2204Y] - BSA and Azide free

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-B MyB (phospho T487) antibody [EPR2204Y] - BSA and Azide free (AB232521)

  • 0
  • 0
Primary Antibodies

AB76009

Anti-B MyB (phospho T487) antibody [EPR2204Y]

20ul selling size|BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-B MyB (phospho T487) antibody [EPR2204Y] (AB76009)

  • 5
  • 1

組合せ製品

Primary Antibodies

AB76009

Anti-B MyB (phospho T487) antibody [EPR2204Y]

20ul selling size|BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-B MyB (phospho T487) antibody [EPR2204Y] (AB76009)

  • 5
  • 1
Primary Antibodies

AB45150

Anti-c-Myb (phospho S11) antibody [EP769Y]

20ul selling size|RabMAb|Recombinant

Western blot - Anti-c-Myb (phospho S11) antibody [EP769Y] (AB45150)

  • 5
  • 2
Primary Antibodies

AB52195

Anti-Cyclin E1 (phospho T395) antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin E1 (phospho T395) antibody (AB52195)

  • 4
  • 1
Primary Antibodies

AB317436

Anti-Cyclin E1 antibody [EPR25394-23]

20ul selling size|Recombinant|RabMAb

Immunocytochemistry/ Immunofluorescence - Anti-Cyclin E1 antibody [EPR25394-23] (AB317436)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Immunogen
精製に関する特記事項
Affinity purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
バッファー組成
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
-20°C
保管に関する情報
Stable for 12 months at -20°C
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

The B MyB protein also known as MYBL2 or B-MYB is a transcription factor involved in cell cycle regulation. It belongs to the MYB family of proteins and has a molecular weight of approximately 92 kDa. This protein is expressed in a range of tissues including proliferating cells. B MyB's activity as a transcription factor involves binding to specific DNA sequences to regulate gene expression essential for cell cycle progression particularly in the S phase where it plays a role in DNA replication and mitosis.
Biological function summary

MYBL2 participates in processes important for normal cell cycle and proliferation. It does not appear to form part of a larger protein complex but regulates genes necessary for cell division independently. B MyB significantly impacts the expression of factors that facilitate the transition from G1 to S phase of the cell cycle impacting cellular growth and replication. Its function ensures that the cells adequately prepare for DNA synthesis and subsequent mitotic division.

Pathways

Various cell cycle regulatory pathways rely on MYBL2 for their correct functioning. B MyB influences the G1/S phase transition and is associated with the E2F transcription factor pathway. In this context it regulates E2F target genes necessary for DNA synthesis. B MyB interacts with other proteins such as CDK2 and cyclins to orchestrate a synchronized cell cycle progression ensuring that cells divide correctly and effectively.

B MyB is frequently associated with cancer development and progression notably in contexts where there is rapid cell proliferation such as in breast cancer and leukemia. Dysregulation of MYBL2 expression or its downstream targets can lead to uncontrolled cell division thereby contributing to oncogenesis. It associates with proteins such as p53 a pivotal tumor suppressor where alterations in their pathways can lead to malignancy. Researchers evaluate B MyB's expression and activity as potential markers for cancer diagnosis and as targets for therapeutic intervention.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Transcription factor involved in the regulation of cell survival, proliferation, and differentiation. Transactivates the expression of the CLU gene.
See full target information MYBL2 pS577 + S581
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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

FEBS letters 594:4266-4279 PubMed32979888

2020

Intramolecular interaction of B-MYB is regulated through Ser-577 phosphorylation.

Applications

Unspecified application

Species

Unspecified reactive species

Eugen Werwein,Abhiruchi Biyanee,Karl-Heinz Klempnauer

Cell death & disease 4:e511 PubMed23449447

2013

B-Myb switches from Cyclin/Cdk-dependent to Jnk- and p38 kinase-dependent phosphorylation and associates with SC35 bodies after UV stress.

Applications

ICC/IF, ICC/IF

Species

Mouse, Human

E Werwein,M Dzuganova,C Usadel,K-H Klempnauer
View all publications
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