Anti-B MyB (phospho S577 + S581) 抗体 (ab61000)
Key features and details
- Rabbit polyclonal to B MyB (phospho S577 + S581)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-B MyB (phospho S577 + S581) antibody
B MyB 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to B MyB (phospho S577 + S581) -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Rat
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免疫原
Synthetic peptide corresponding to Human B MyB (phospho S577 + S581).
Database link: P10244 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Affinity purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab61000の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/1000. Detects a band of approximately 79 kDa (predicted molecular weight: 79 kDa).
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| 特記事項 |
|---|
|
WB
1/500 - 1/1000. Detects a band of approximately 79 kDa (predicted molecular weight: 79 kDa). |
ターゲット情報
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機能
Transcription factor involved in the regulation of cell survival, proliferation, and differentiation. Transactivates the expression of the CLU gene. -
配列類似性
Contains 3 HTH myb-type DNA-binding domains. -
翻訳後修飾
Phosphorylated by cyclin A/CDK2 during S-phase. Phosphorylation at Thr-520 is probably involved in transcriptional activity. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 4605 Human
- Entrez Gene: 296344 Rat
- Omim: 601415 Human
- SwissProt: P10244 Human
- Unigene: 179718 Human
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別名
- B-Myb antibody
- BMyB antibody
- MGC15600 antibody
see all
画像
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Western blot - Anti-B MyB (phospho S577 + S581) antibody (ab61000)All lanes : Anti-B MyB (phospho S577 + S581) antibody (ab61000) at 1/500 dilution
Lane 1 : extracts from K562 cells
Lane 2 : extracts from K562 cells with immunizing phosphopeptide
Predicted band size: 79 kDa
Observed band size: 79 kDa
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab61000 は 2 報の論文で使用されています。
- Werwein E et al. Intramolecular interaction of B-MYB is regulated through Ser-577 phosphorylation. FEBS Lett 594:4266-4279 (2020). PubMed: 32979888
- Werwein E et al. B-Myb switches from Cyclin/Cdk-dependent to Jnk- and p38 kinase-dependent phosphorylation and associates with SC35 bodies after UV stress. Cell Death Dis 4:e511 (2013). ICC/IF ; Mouse, Human . PubMed: 23449447