Anti-AZI1 抗体 (ab84864)
Key features and details
- Rabbit polyclonal to AZI1
- Suitable for: ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-AZI1 antibody
AZI1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to AZI1 -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Chimpanzee, Gorilla, Orangutan
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免疫原
Synthetic peptide, corresponding to a region between residue 1033 and 1083 of human AZI1 (EAW89632.1)
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ポジティブ･コントロール
- HeLa Cells.
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特記事項
Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Purified using an epitope specific to AZI1 immobilized on solid support. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab84864の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
| 特記事項 |
|---|
追加情報
ICC/IF: 1/100 - 1/500. Formaldehyde-Fixed or Acetone fixed cells.
Permeabilization with Triton-X 100 is recommended.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Permeabilization with Triton-X 100 is recommended.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
ターゲット情報
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機能
May play a role in spermatogenesis. -
配列類似性
Contains 1 IQ domain. -
翻訳後修飾
Phosphorylated upon DNA damage, probably by ATM or ATR. -
細胞内局在
Cytoplasm > cytoskeleton > centrosome. - Information by UniProt
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参照データベース
- Entrez Gene: 22994 Human
- Omim: 613479 Human
- SwissProt: Q9UPN4 Human
- Unigene: 514578 Human
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別名
- 5 azacytidine induced 1 antibody
- 5-azacytidine-induced protein 1 antibody
- AZ1 antibody
see all
画像
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Immunocytochemistry/ Immunofluorescence - Anti-AZI1 antibody (ab84864)ab84864 at 1/250 dilution staining AZI1 in asynchronous HeLa cells by Immunocytochemistry, NBF-fixed. Detection: Red-fluorescent goat anti-rabbit IgG highly cross-adsorbed Antibody Hilyte PlusTM 555 used at a dilution of 1/100.
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Immunocytochemistry/ Immunofluorescence - Anti-AZI1 antibody (ab84864)ab84864 at 1/250 dilution staining AZI1 in asynchronous HeLa cells by Immunocytochemistry, NBF-fixed. Detection: Red-fluorescent goat anti-rabbit IgG highly cross-adsorbed Antibody Hilyte PlusTM 555 used at a dilution of 1/100.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (10)
ab84864 は 10 報の論文で使用されています。
- Wang J et al. CEP131 knockdown inhibits cell proliferation by inhibiting the ERK and AKT signaling pathways in non-small cell lung cancer. Oncol Lett 19:3145-3152 (2020). PubMed: 32218865
- Kyun ML et al. Wnt3a Stimulation Promotes Primary Ciliogenesis through ß-Catenin Phosphorylation-Induced Reorganization of Centriolar Satellites. Cell Rep 30:1447-1462.e5 (2020). PubMed: 32023461
- Holdgaard SG et al. Selective autophagy maintains centrosome integrity and accurate mitosis by turnover of centriolar satellites. Nat Commun 10:4176 (2019). PubMed: 31519908
- Krastev DB et al. Coupling bimolecular PARylation biosensors with genetic screens to identify PARylation targets. Nat Commun 9:2016 (2018). PubMed: 29789535
- Nielsen JC et al. Osmotic Stress Blocks Mobility and Dynamic Regulation of Centriolar Satellites. Cells 7:N/A (2018). PubMed: 29932434
- Hoang-Minh LB et al. PCM1 Depletion Inhibits Glioblastoma Cell Ciliogenesis and Increases Cell Death and Sensitivity to Temozolomide. Transl Oncol 9:392-402 (2016). ICC/IF ; Human . PubMed: 27661404
- Tollenaere MA et al. p38- and MK2-dependent signalling promotes stress-induced centriolar satellite remodelling via 14-3-3-dependent sequestration of CEP131/AZI1. Nat Commun 6:10075 (2015). IP ; Human . PubMed: 26616734
- Staples CJ et al. Ccdc13 is a novel human centriolar satellite protein required for ciliogenesis and genome stability. J Cell Sci 127:2910-9 (2014). ICC/IF . PubMed: 24816561
- Villumsen BH et al. A new cellular stress response that triggers centriolar satellite reorganization and ciliogenesis. EMBO J 32:3029-40 (2013). PubMed: 24121310
- Staples CJ et al. The centriolar satellite protein Cep131 is important for genome stability. J Cell Sci 125:4770-9 (2012). PubMed: 22797915