Anti-Axl 抗体 [EPR19880]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Axl antibody [EPR19880] (AB219651)

Immunohistochemical analysis of paraffin-embedded human lung tissue labeling Axl with ab219651 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Sporadic cytoplasmic staining on human lung.

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Axl antibody [EPR19880] (AB219651)

Immunohistochemical analysis of paraffin-embedded human adipose tissue labeling Axl with ab219651 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Negative control : No staining on human adipose tissues (PMID : 26573603).

Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Axl antibody [EPR19880] (AB219651)

Immunohistochemical analysis of paraffin-embedded human breast and breast carcinoma tissue labeling Axl with ab219651 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on cancer cells of the human breast carcinoma, and the pericarcinous tissue was nearly negative (magnification x150) (PMID : 25337673).

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Axl antibody [EPR19880] (AB219651)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue labeling Axl with ab219651 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on cancer cells of human lung carcinoma (PMID : 25337673).

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• WB

Lab

Western blot - Anti-Axl antibody [EPR19880] (AB219651)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

ab219651 exhibits low sensitivity in western blot. It is recommended to use ab259831 or ab215205 as alternatives or to optimize experimental conditions by increasing the sample loading amount, using a lower antibody dilution ratio, and employing femtogram-level sensitivity substrates.

Lanes 1 - 3:

Western blot - Anti-Axl antibody [EPR19880] (ab219651) at 1/1000 dilution

Lanes 5 - 7:

Western blot - Anti-Axl antibody [EPR23892-15] (<a href='/products/primary-antibodies/axl-antibody-epr23892-15-ab259831'>ab259831</a>) at 1/1000 dilution

Lanes 9 - 11:

Western blot - Anti-Axl antibody [EPR21107] (<a href='/products/primary-antibodies/axl-antibody-epr21107-ab215205'>ab215205</a>) at 1/1000 dilution

Lanes 1, 5 and 9:

HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 20 µg

Lanes 2, 6 and 9:

HUVEC (human umbilical vein endothelial cell line) whole cell lysate 20 µg

Lanes 3, 7 and 11:

A549 (Human lung carcinoma epithelial cell) whole cell lysate 20 µg

Lanes 4, 8 and 12:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 98 kDa

Observed band size: 80 kDa,140 kDa

false

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-Axl antibody [EPR19880] (AB219651)

All lanes:

Western blot - Anti-AXL (phospho Y702) + MERTK (phospho Y753) + TYRO3 (phospho Y685) antibody [EPR26140-66] (<a href='/products/primary-antibodies/axl-phospho-y702-mertk-phospho-y753-tyro3-phospho-y685-antibody-epr26140-66-ab302718'>ab302718</a>) at 1/1000 dilution

Lane 1:

Untreated NCI-H1299 whole cell lysate (Untreated membrane)

Lane 2:

NCI-H1299 treated with 100 ng/ml Gas6 for 10 minutes, whole cell lysate (Untreated membrane)

Lane 3:

NCI-H1299 treated with 10mM pervanadate for 10 minutes, whole cell lysate (Untreated membrane) at 40 µg

Lane 4:

NCI-H1299 treated with 100 ng/ml Gas6 and 10mM pervanadate for 10 minutes, whole cell lysate (Untreated membrane) at 40 µg

Lane 5:

Untreated NCI-H1299 whole cell lysate (Phosphatase treated membrane)

Lane 6:

NCI-H1299 treated with 100 ng/ml Gas6 for 10 minutes, whole cell lysate (Phosphatase treated membrane)

Lane 7:

NCI-H1299 treated with 10mM pervanadate for 10 minutes, whole cell lysate (Phosphatase treated membrane)

Lane 8:

NCI-H1299 treated with 100 ng/ml Gas6 and 10mM pervanadate for 10 minutes, whole cell lysate (Phosphatase treated membrane)

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 138 kDa

true

Exposure time: 136s

• WB

Supplier Data

Western blot - Anti-Axl antibody [EPR19880] (AB219651)

Blocking/Dilution buffer : 5% NFDM/TBST.

Detection of endogenous expression of Axl in WB will need optimization. Under our experimental conditions we could not detect endogenous Axl in human cell and tissue lysates.

All lanes:

Western blot - Anti-Axl antibody [EPR19880] (ab219651) at 1/10000 dilution

Lane 1:

293T (Human epithelial cell line from embryonic kidney) transfected with an empty expression vector, whole cell lysate at 10 µg

Lane 2:

293T (Human epithelial cell line from embryonic kidney) transfected with a His-tagged human Axl construct whole cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase at 1/100000 dilution

Predicted band size: 98 kDa

Observed band size: 138 kDa

false

Exposure time: 5s

• WB

Supplier Data

Western blot - Anti-Axl antibody [EPR19880] (AB219651)

All lanes:

Western blot - Anti-AXL (phospho Y702) + MERTK (phospho Y753) + TYRO3 (phospho Y685) antibody [EPR26140-66] (<a href='/products/primary-antibodies/axl-phospho-y702-mertk-phospho-y753-tyro3-phospho-y685-antibody-epr26140-66-ab302718'>ab302718</a>) at 1/1000 dilution

Lane 1:

Untreated 293T cells transfected with a human AXL expression vector containing a myc-His-tag® lysate

Lane 2:

Gas6/pervanadate treated 293T cells transfected with a human AXL expression vector containing a myc-His-tag®

Lane 3:

Untreated 293T cells transfected with a human AXL(Y702A mutant) expression vector containing a myc-His-tag® lysate

Lane 4:

Gas6/pervanadate 293T cells transfected with a human AXL(Y702A mutant) expression vector containing a myc-His-tag®

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 138 kDa

false

Exposure time: 180s