Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
リコンビナントRabMAb

Anti-Axin 2 抗体 [EPR2005(2)] (ab109307)

Reviews (5) Submit a question References (58)
Western blot - Anti-Axin 2 antibody [EPR2005(2)] (ab109307)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Axin 2 antibody [EPR2005(2)] (ab109307)
  • Immunocytochemistry/ Immunofluorescence - Anti-Axin 2 antibody [EPR2005(2)] (ab109307)
  • Western blot - Anti-Axin 2 antibody [EPR2005(2)] (ab109307)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Axin 2 antibody [EPR2005(2)] (ab109307)
  • Western blot - Anti-Axin 2 antibody [EPR2005(2)] (ab109307)
  • Immunocytochemistry/ Immunofluorescence - Anti-Axin 2 antibody [EPR2005(2)] (ab109307)
  • Immunocytochemistry/ Immunofluorescence - Anti-Axin 2 antibody [EPR2005(2)] (ab109307)
  • Anti-Axin 2 antibody [EPR2005(2)] (ab109307)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2005(2)] to Axin 2
  • Suitable for: ICC/IF, IHC-P, WB
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Alexa Fluor® 750 Carrier Free

製品の概要

  • 製品名

    Anti-Axin 2 antibody [EPR2005(2)]
    Axin 2 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR2005(2)] to Axin 2

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: ICC/IF, IHC-P, WBmore details

  • 種交差性

    交差種: Mouse, Rat, Human
    交差が予測される動物種: Pig

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: C6, PC-12, NIH/3T3, MCF7, SW480, and PC3 cell lysates. IHC-P: Human lung carcinoma tissue. ICC/IF: LnCap cells.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab109307の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ICC/IF (2)
1/150.
IHC-P (2)
1/150. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB (1)
1/1000 - 1/2000. Detects a band of approximately 95 kDa (predicted molecular weight: 94 kDa).
特記事項
ICC/IF
1/150.
IHC-P
1/150. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
1/1000 - 1/2000. Detects a band of approximately 95 kDa (predicted molecular weight: 94 kDa).

ターゲット情報

  • 機能

    Inhibitor of the Wnt signaling pathway. Down-regulates beta-catenin. Probably facilitate the phosphorylation of beta-catenin and APC by GSK3B.

  • 組織特異性

    Expressed in brain and lymphoblast.

  • 関連疾患

    Defects in AXIN2 are involved in colorectal cancer (CRC) [MIM:114500]. They appear to be specifically associated with defective mismatch repair.
    Defects in AXIN2 are the cause of oligodontia-colorectal cancer syndrome (ODCRCS) [MIM:608615]. Affected individuals manifest severe tooth agenesis and colorectal cancer or precancerous lesions of variable types.

  • 配列類似性

    Contains 1 DIX domain.
    Contains 1 RGS domain.

  • ドメイン

    The tankyrase-binding motif (also named TBD) is required for interaction with tankyrase TNKS and TNKS2.

  • 翻訳後修飾

    Probably phosphorylated by GSK3B and dephosphorylated by PP2A.
    ADP-ribosylated by tankyrase TNKS and TNKS2. Poly-ADP-ribosylated protein is recognized by RNF146, followed by ubiquitination and subsequent activation of the Wnt signaling pathway.
    Ubiquitinated by RNF146 when poly-ADP-ribosylated, leading to its degradation and subsequent activation of the Wnt signaling pathway. Deubiquitinated by USP34, deubiquitinated downstream of beta-catenin stabilization step: deubiquitination is important Wnt signaling to positively regulate beta-catenin (CTNBB1)-mediated transcription.

  • 細胞内局在

    Cytoplasm.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • Axil antibody
    • Axin like protein antibody
    • Axin-2 antibody
    • Axin-like protein antibody
    • Axin2 antibody
    • AXIN2_HUMAN antibody
    • Axis inhibition protein 2 antibody
    • Conductin antibody
    • DKFZp781B0869 antibody
    • MGC10366 antibody
    • MGC126582 antibody
    see all

画像

  • Western blot - Anti-Axin 2 antibody [EPR2005(2)] (ab109307)
    Western blot - Anti-Axin 2 antibody [EPR2005(2)] (ab109307)
    All lanes : Anti-Axin 2 antibody [EPR2005(2)] (ab109307) at 1/1000 dilution (unpurified)

    Lane 1 : SW480 cell lysate
    Lane 2 : C6 cell lysate
    Lane 3 : PC-12 cell lysate
    Lane 4 : NIH/3T3 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 94 kDa
    Observed band size: 94 kDa



    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Axin 2 antibody [EPR2005(2)] (ab109307)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Axin 2 antibody [EPR2005(2)] (ab109307)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue labelling Axin 2 with unpurified ab109307 at 1/150. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

  • Immunocytochemistry/ Immunofluorescence - Anti-Axin 2 antibody [EPR2005(2)] (ab109307)
    Immunocytochemistry/ Immunofluorescence - Anti-Axin 2 antibody [EPR2005(2)] (ab109307)This image is courtesy of an anonymous Abreview

    Unpurified ab109307 staining Axin 2 in 293T cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 1% Triton X-100 and blocked with 3% BSA for 1 hour at room temperature. Samples were incubated with primary antibody (1/50 in PBS + 3% BSA) for 16 hours. An Alexa Fluor® 488-conjugated donkey anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.

    See Abreview

  • Western blot - Anti-Axin 2 antibody [EPR2005(2)] (ab109307)
    Western blot - Anti-Axin 2 antibody [EPR2005(2)] (ab109307)
    All lanes : Anti-Axin 2 antibody [EPR2005(2)] (ab109307) at 1/1000 dilution (purified)

    Lane 1 : SW480 cell lysate
    Lane 2 : C6 cell lysate
    Lane 3 : PC-12 cell lysate
    Lane 4 : NIH/3T3 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 94 kDa
    Observed band size: 94 kDa



    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Axin 2 antibody [EPR2005(2)] (ab109307)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Axin 2 antibody [EPR2005(2)] (ab109307)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue labelling Axin 2 with purified ab109307 at 1/150. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

  • Western blot - Anti-Axin 2 antibody [EPR2005(2)] (ab109307)
    Western blot - Anti-Axin 2 antibody [EPR2005(2)] (ab109307)
    All lanes : Anti-Axin 2 antibody [EPR2005(2)] (ab109307) at 1/1000 dilution (unpurified)

    Lane 1 : MCF7 cell lysates
    Lane 2 : SW480 cell lysates
    Lane 3 : PC3 cell lysates

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 94 kDa
    Observed band size: 95 kDa why is the actual band size different from the predicted?

  • Immunocytochemistry/ Immunofluorescence - Anti-Axin 2 antibody [EPR2005(2)] (ab109307)
    Immunocytochemistry/ Immunofluorescence - Anti-Axin 2 antibody [EPR2005(2)] (ab109307)

    Immunocytochemistry/Immunofluorescence analysis of LnCap cells labelling Axin 2 with unpurified ab109307 at 1/150. Cells were fixed with 4% paraformaldehyde. An Alexa Fluor® 555-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain.

    Control: primary antibody (1/150) and secondary antibody, ab150113, an Alexa Fluor® 488-conjugated goat anti-mouse IgG (1/500).

  • Immunocytochemistry/ Immunofluorescence - Anti-Axin 2 antibody [EPR2005(2)] (ab109307)
    Immunocytochemistry/ Immunofluorescence - Anti-Axin 2 antibody [EPR2005(2)] (ab109307)

    Immunocytochemistry/Immunofluorescence analysis of LnCap cells labelling Axin 2 with purified ab109307 at 1/150. Cells were fixed with 4% paraformaldehyde. An Alexa Fluor® 555-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain.

    Control: primary antibody (1/150) and secondary antibody, ab150113, an Alexa Fluor® 488-conjugated goat anti-mouse IgG (1/500).

  • Anti-Axin 2 antibody [EPR2005(2)] (ab109307)
    Anti-Axin 2 antibody [EPR2005(2)] (ab109307)

参考文献 (58)

ab109307 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab109307 は 58 報の論文で使用されています。

  • He T  et al. Suppression of preadipocyte determination by SOX4 limits white adipocyte hyperplasia in obesity. iScience 26:106289 (2023). PubMed: 36968079
  • Wong TL  et al. ADAR1-mediated RNA editing of SCD1 drives drug resistance and self-renewal in gastric cancer. Nat Commun 14:2861 (2023). PubMed: 37208334
  • Ou Z  et al. Hypoxia mediates immune escape of pancreatic cancer cells by affecting miR-1275/AXIN2 in natural killer cells. Front Immunol 14:1271603 (2023). PubMed: 38035113
  • Lukosevicius R  et al. miRNome Profiling and Functional Analysis Reveal Involvement of hsa-miR-1246 in Colon Adenoma-Carcinoma Transition by Targeting AXIN2 and CFTR. Int J Mol Sci 23:N/A (2022). PubMed: 35216222
  • Zhou L  et al. The E3 Ubiquitin Ligase TRIM11 Facilitates Gastric Cancer Progression by Activating the Wnt/β-Catenin Pathway via Destabilizing Axin1 Protein. J Oncol 2022:8264059 (2022). PubMed: 35237324
View all Publications for this product

レビューと Q&A

Show All レビュー Q&A
レビューを送る 質問を送る

1-5 of 5 Abreviews or Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Axin 2 antibody [EPR2005(2)]

 Average
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (MDA-MB-231 breast cancer xenograft)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9
Permeabilization
No
Specification
MDA-MB-231 breast cancer xenograft
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 15% · Temperature: 20°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Dec 14 2015

Immunocytochemistry/ Immunofluorescence abreview for Anti-Axin 2 antibody [EPR2005(2)]

 Excellent
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Sample
Human Cell (293T)
Specification
293T
Permeabilization
Yes - Triton 1%
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Nov 20 2014

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Axin 2 antibody [EPR2005(2)]

 Good
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
(agent) for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate pH6
Sample
Mouse Tissue sections (MOUSE EMBRYO 20DPC)
Specification
MOUSE EMBRYO 20DPC
Permeabilization
No
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Sep 09 2013

Western blot abreview for Anti-Axin 2 antibody [EPR2005(2)]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Pig Cell lysate - whole cell (PAE cell line (Pig Aortic Endothelial))
Loading amount
70 µg
Specification
PAE cell line (Pig Aortic Endothelial)
Gel Running Conditions
Reduced Denaturing (10)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Raul Peña

Verified customer

投稿 Dec 17 2012

Immunocytochemistry/ Immunofluorescence abreview for Anti-Axin 2 antibody [EPR2005(2)]

 Good
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Embryonic Stem Cells)
Specification
Embryonic Stem Cells
Fixative
Paraformaldehyde
Permeabilization
Yes - PBS 0,3% triton
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MRS. XXXXXX XXXXXX

Verified customer

投稿 Nov 27 2012

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com