Anti-Axin 2 抗体 (ab32197)
Key features and details
- Rabbit polyclonal to Axin 2
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
-
製品名
Anti-Axin 2 antibody
Axin 2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Axin 2 -
由来種
Rabbit -
特異性
Milk blocking not recommended -
アプリケーション
適用あり: IHC-P, WBmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Chicken -
免疫原
Synthetic peptide corresponding to Mouse Axin 2 aa 800 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab32196) -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
-
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Conjugation kits
-
Isotype control
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab32197の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
IHC-P | (5) |
Use a concentration of 0.1 - 1 µg/ml.
|
WB | (4) |
Use a concentration of 1 µg/ml. Detects a band of approximately 94 kDa (predicted molecular weight: 94 kDa) in endogenous lysates. Ab32197 gave a positive signal against the tagged partial recombinant protein which has an expected molecular weight of 37 kDa.
|
特記事項 |
---|
IHC-P
Use a concentration of 0.1 - 1 µg/ml. |
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 94 kDa (predicted molecular weight: 94 kDa) in endogenous lysates. Ab32197 gave a positive signal against the tagged partial recombinant protein which has an expected molecular weight of 37 kDa. |
ターゲット情報
-
機能
Inhibitor of the Wnt signaling pathway. Down-regulates beta-catenin. Probably facilitate the phosphorylation of beta-catenin and APC by GSK3B. -
組織特異性
Expressed in brain and lymphoblast. -
関連疾患
Defects in AXIN2 are involved in colorectal cancer (CRC) [MIM:114500]. They appear to be specifically associated with defective mismatch repair.
Defects in AXIN2 are the cause of oligodontia-colorectal cancer syndrome (ODCRCS) [MIM:608615]. Affected individuals manifest severe tooth agenesis and colorectal cancer or precancerous lesions of variable types. -
配列類似性
Contains 1 DIX domain.
Contains 1 RGS domain. -
ドメイン
The tankyrase-binding motif (also named TBD) is required for interaction with tankyrase TNKS and TNKS2. -
翻訳後修飾
Probably phosphorylated by GSK3B and dephosphorylated by PP2A.
ADP-ribosylated by tankyrase TNKS and TNKS2. Poly-ADP-ribosylated protein is recognized by RNF146, followed by ubiquitination and subsequent activation of the Wnt signaling pathway.
Ubiquitinated by RNF146 when poly-ADP-ribosylated, leading to its degradation and subsequent activation of the Wnt signaling pathway. Deubiquitinated by USP34, deubiquitinated downstream of beta-catenin stabilization step: deubiquitination is important Wnt signaling to positively regulate beta-catenin (CTNBB1)-mediated transcription. -
細胞内局在
Cytoplasm. - Information by UniProt
-
参照データベース
- Entrez Gene: 8313 Human
- Entrez Gene: 12006 Mouse
- Entrez Gene: 29134 Rat
- Omim: 604025 Human
- SwissProt: Q9Y2T1 Human
- SwissProt: O88566 Mouse
- SwissProt: O70240 Rat
- Unigene: 156527 Human
see all -
別名
- Axil antibody
- Axin like protein antibody
- Axin-2 antibody
see all
画像
-
Anti-Axin 2 antibody (ab32197) at 1 µg/ml + AXIN2 partial recombinant tagged protein at 0.1 µg
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 94 kDaab32197 gave a positive signal against the tagged partial recombinant protein which has an expected molecular weight of 37 kDa.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Axin 2 antibody (ab32197)IHC image of ab32197 stained area of normal tissue in a human colorectal cancer FFPE section. Section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30 seconds at 125°C. Section was incubated with ab32197 at a dilution of 1:500 for 1h at room temperature and detected using an HRP conjugated polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
-
All lanes : Anti-Axin 2 antibody (ab32197) at 1 µg/ml
Lane 1 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate
Lane 2 : Colon (Mouse) Tissue Lysate
Lane 3 : HCT 116 (Human Colorectal Carcinoma) Whole Cell Lysate
Lane 4 : LS147T (Colon cancer cells) (Human) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) (ab65484) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 94 kDa
Observed band size: 94 kDa
Additional bands at: 32 kDa, 75 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 16 minutes
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (92)
ab32197 は 92 報の論文で使用されています。
- Sun L et al. Bile salt hydrolase in non-enterotoxigenic Bacteroides potentiates colorectal cancer. Nat Commun 14:755 (2023). PubMed: 36765047
- Bala P et al. Aberrant cell state plasticity mediated by developmental reprogramming precedes colorectal cancer initiation. Sci Adv 9:eadf0927 (2023). PubMed: 36989360
- Kagioka T et al. Lithium carbonate accelerates the healing of apical periodontitis. Sci Rep 13:7886 (2023). PubMed: 37193735
- Huang B et al. Scutellarin ameliorates diabetic nephropathy via TGF-β1 signaling pathway. Biol Pharm Bull N/A:N/A (2023). PubMed: 37853616
- Liu CT et al. Inhibition of β-catenin signaling attenuates arteriovenous fistula thickening in mice by suppressing myofibroblasts. Mol Med 28:7 (2022). PubMed: 35062862