Anti-Avidin 抗体

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Biological function summary

Avidin binds biotin with high specificity and affinity forming one of the strongest known non-covalent interactions. Avidin is not usually part of a cellular complex but functions independently as a biotin-binding molecule. It plays a role in the regulation of biotin availability which is a critical cofactor for carboxylase reactions. The availability of biotin affects various metabolic processes since biotin is necessary for enzymes that play roles in gluconeogenesis fatty acid synthesis and the catabolism of branched-chain amino acids.

Pathways

The strong avidin-biotin binding is exploited in pathways related to signal amplification and detection. Avidin often in combination with peroxidase conjugates enhances the sensitivity and precision in biotechnological applications like ELISA and Western blot through its interaction with biotinylated molecules. Avidin's interaction with biotinylated chemical species allows cleaning and enrichment of biotinylated proteins from biological samples. Though avidin itself is not directly involved in cellular pathways the avidin-biotin system is instrumental for tagging and detecting molecular interactions.