Anti-Avian Influenza Nucleoprotein 抗体 (ab25921)
Key features and details
- Rabbit polyclonal to Avian Influenza Nucleoprotein
- Suitable for: WB
- Reacts with: Influenza A
- Isotype: IgG
製品の概要
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製品名
Anti-Avian Influenza Nucleoprotein antibody
Avian Influenza Nucleoprotein 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Avian Influenza Nucleoprotein -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Influenza A -
免疫原
Synthetic peptide corresponding to Avian Influenza Nucleoprotein aa 58-77.
Sequence:KLSDYEGRLIQNSITIERMV
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ポジティブ・コントロール
- Recombinant fusion protein of avian flu nucleoprotein.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
バッファー
pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: 0.05% BSA, PBS -
Concentration information loading...
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精製度
Protein G purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab25921の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
特記事項 |
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Please note from the image legend that a recombinant fusion protein was used to generate the western blot image.
The molecular weight shown is the molecular weight of the fusion protein (e.g nucleoprotein amino acids 58-77 + fusion protein) not the Avian Influenza Nucleoprotein.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
ターゲット情報
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関連性
Encapsidates the negative strand viral RNA, protecting it from nucleases. The encapsidated genomic RNA is termed the ribonucleoprotein (RNP) and serves as template for transcription and replication. The RNP needs to be localized in the nucleus to start an infectious cycle, but is too large to diffuse through the nuclear pore complex. NP comprises at least 2 nuclear localization signals and is responsible of the active RNP import into the nucleus through the cellular importin alpha/beta pathway. Later in the infection, nucleus export of RNP are mediated through viral proteins NEP interacting with M1 which binds nucleoproteins. It is possible that the nucleoprotein binds directly exportin-1 (XPO1) and plays an active role in RNP nuclear export. M1 interaction with RNP seems to hide nucleoprotein's nuclear localization signals. Soon after a virion infects a new cell, M1 dissociates from the RNP under acidification of the virion driven by M2 protein. Dissociation of M1 from RNP unmask nucleoprotein's nuclear localization signals, targeting the RNP to the nucleus -
細胞内局在
Nuclear -
参照データベース
- SwissProt: P68881 Influenza A
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別名
- NP antibody
- Nucleocapsid protein antibody
- Nucleoprotein antibody
- Protein N antibody
画像
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All lanes : Anti-Avian Influenza Nucleoprotein antibody (ab25921) at 0.5 µg/ml
Lane 1 : Recombinant fusion protein containing amino acids 58-77 of Avian flu nucleoprotein at 0.1 µg
Lane 2 : Fusion partner without these amino acids
Observed band size: 31 kDa why is the actual band size different from the predicted?
Additional bands at: 56 kDa, 59 kDa, 66 kDa. We are unsure as to the identity of these extra bands.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab25921 は論文での使用が確認できていません。