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AB25921

Anti-Avian Influenza Nucleoprotein 抗体

Anti-Avian Influenza Nucleoprotein antibody

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(1 Publication)

Rabbit Polyclonal NCAP antibody. Suitable for WB and reacts with Recombinant full length protein - Influenza A samples. Cited in 1 publication.

別名を表示する

Nucleoprotein, Nucleocapsid protein, Protein N, NP

1 Images
Western blot - Anti-Avian Influenza Nucleoprotein antibody (AB25921)
  • WB

Unknown

Western blot - Anti-Avian Influenza Nucleoprotein antibody (AB25921)

All lanes:

Western blot - Anti-Avian Influenza Nucleoprotein antibody (ab25921) at 0.5 µg/mL

Lane 1:

Recombinant fusion protein containing amino acids 58-77 of Avian flu nucleoprotein at 0.1 µg

Lane 2:

Fusion partner without these amino acids

Observed band size: 31 kDa,56 kDa,59 kDa,66 kDa

false

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

No

交差種

Influenza A

アプリケーション

WB

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Influenza A": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Recombinant full length protein - Influenza A": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "<p></p>" } } }
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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein G
バッファー組成
pH: 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 0.05% BSA
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

The Avian Influenza Nucleoprotein also known as NP is a structural protein found in influenza viruses particularly avian strains. It functions mechanically as a part of the ribonucleoprotein (RNP) complex encapsulating the viral RNA in a helical structure therefore facilitating its replication and transcription. The molecular weight of the nucleoprotein is approximately 56 kDa. It expresses predominantly within the host cell's cytoplasm participating in viral assembly and eventually packaging.
Biological function summary

The nucleoprotein functions as an important player in viral replication and interaction with host cell machinery. Within the RNP complex it binds to viral RNA and polymerase subunits ensuring the stability of viral genome replication. Additionally the nucleoprotein interacts with host nuclear import and export pathways to shuttle viral components across the nuclear membrane efficiently important for viral propagation within host cells.

Pathways

Avian Influenza Nucleoprotein influences viral RNA synthesis and export pathways. It associates with viral polymerase proteins such as PA PB1 and PB2 during the viral RNA replication process. Moreover its interaction with nuclear export factors like exportin-1 (CRM1) integrates it into the host's nuclear export pathway facilitating the movement of viral RNA from nucleus to cytoplasm which is essential for the completion of the viral life cycle.

Avian Influenza Nucleoprotein is central to the pathogenicity of avian influenza infections which can result in avian influenza virus (AIV) outbreaks. This relationship with AIV highlights its importance in zoonotic transmission and potential emergence of avian influenza in humans. Its association with proteins involved in the immune response such as interferon-inducible proteins also highlights its role in modulating host immune evasion a factor that complicates the control of influenza outbreaks.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Encapsidates the negative strand viral RNA, protecting it from nucleases. The encapsidated genomic RNA is termed the ribonucleoprotein (RNP) and serves as template for transcription and replication. The RNP needs to be localized in the host nucleus to start an infectious cycle, but is too large to diffuse through the nuclear pore complex. NP comprises at least 2 nuclear localization signals that are responsible for the active RNP import into the nucleus through cellular importin alpha/beta pathway. Later in the infection, nclear export of RNPs are mediated through viral proteins NEP interacting with M1 which binds nucleoproteins. It is possible that nucleoprotein binds directly host exportin-1/XPO1 and plays an active role in RNPs nuclear export. M1 interaction with RNP seems to hide nucleoprotein's nuclear localization signals. Soon after a virion infects a new cell, M1 dissociates from the RNP under acidification of the virion driven by M2 protein. Dissociation of M1 from RNP unmasks nucleoprotein's nuclear localization signals, targeting the RNP to the nucleus.
See full target information NP
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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in microbiology 13:1042916 PubMed36458187

2022

A replication-deficient H9N2 influenza virus carrying H5 hemagglutinin conferred protection against H9N2 and H5N1 influenza viruses in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Weigang Ren,Shuli Pei,Wenming Jiang,Meixia Zhao,Le Jiang,Honggang Liu,Yongxiang Yi,Mizhou Hui,Junwei Li
View all publications
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