Anti-Avian Influenza A Hemagglutinin 抗体 (ab62587)
Key features and details
- Goat polyclonal to Avian Influenza A Hemagglutinin
- Suitable for: ELISA, WB
- Isotype: IgG
製品の概要
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製品名
Anti-Avian Influenza A Hemagglutinin antibody
Avian Influenza A Hemagglutinin 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to Avian Influenza A Hemagglutinin -
由来種
Goat -
特異性
ab62587 can be used for the detection of the Hemagglutinin protein from the H5N1 strain of avian influenza A in ELISA and WB. It will detect 10 ng of free peptide at 1 μg/mL.
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アプリケーション
適用あり: ELISA, WBmore details -
種交差性
交差種: H5N1 subtype -
免疫原
Synthetic peptide corresponding to 12 amino acids near the amino terminus of the Avian influenza A Hemagglutinin 1 protein. Efforts were made to use relatively conserved regions of the viral sequence as the antigen.
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ポジティブ･コントロール
- WB: H5N1 Hemagglutinin recombinant protein. ELISA: H5N1 Hemagglutinin recombinant protein.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab62587の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ELISA | ||
| WB |
| 特記事項 |
|---|
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 64 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
ターゲット情報
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関連性
Influenza A virus is a major public health threat, killing more than 30,000 people per year in the USA. Novel influenza virus strains caused by genetic drift and viral recombination emerge periodically to which humans have little or no immunity, resulting in devastating pandemics. Influenza A can exist in a variety of animals; however it is in birds that all subtypes can be found. These subtypes are classified based on the combination of the virus coat glycoproteins hemagglutinin (HA) and neuraminidase (NA) subtypes. HA interacts with cell surface proteins containing oligosaccharides with terminal sialyl residues. Virus isolated from a human infected with the H5N1 strain in 1997 could bind to oligosaccharides from human as well as avian sources, indicating its species-jumping ability. -
細胞内局在
Cell Membrane -
別名
- HA1 antibody
- HA2 antibody
- Hemagglutinin antibody
see all
画像
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Western blot - Anti-Avian Influenza A Hemagglutinin antibody (ab62587)Lane 1 : Anti-Avian Influenza A Hemagglutinin antibody (ab62587) at 0.5 µg/ml
Lane 2 : Anti-Avian Influenza A Hemagglutinin antibody (ab62587) at 1 µg/ml
All lanes : 30 ng of H5N1 Hemagglutinin recombinant protein
Secondary
All lanes : Rabbit anti-goat IgG HRP conjugate at 1/10000 dilution
Predicted band size: 64 kDa1h incubation at RT in 5% NFDM/TBST.
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ELISA - Anti-Avian Influenza A Hemagglutinin antibody (ab62587)Validation with H5N1 HA Protein Coating Antigen: H5N1 Hemagglutinin recombinant protein, 2 μg/mL, incubated at 4 ˚C overnight. Detection Antibodies: ab62587, dilution: 0.3-1000 ng/mL, incubated at RT for 1 hr. Secondary Antibodies: Rabbit anti-goat HRP at 1/10000, incubated at RT for 1 hr.
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Western blot - Anti-Avian Influenza A Hemagglutinin antibody (ab62587)All lanes : Anti-Avian Influenza A Hemagglutinin antibody (ab62587) at 1 µg/ml
Lane 1 : 5 ng of Recombinant HA1
Lane 2 : 25 ng of Recombinant HA1
Predicted band size: 64 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?
Additional bands at: 33 kDa, 35 kDa, 40 kDa. We are unsure as to the identity of these extra bands.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab62587 は論文での使用が確認できていません。