Anti-Aurora B 抗体 (ab70238)
Key features and details
- Rabbit polyclonal to Aurora B
- Suitable for: WB, IP, ICC
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Aurora B antibody
Aurora B 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Aurora B -
由来種
Rabbit -
アプリケーション
適用あり: WB, IP, ICCmore details -
種交差性
交差種: Human
交差が予測される動物種: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
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免疫原
Synthetic peptide corresponding to Human Aurora B (C terminal).
Database link: Q96GD4 -
ポジティブ･コントロール
- IP: Lysate from nocodazole treated HeLa cells; WB: Lysates from nocodazole treated HeLa cells; ICC: HeLa cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab70238の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/5000 - 1/15000. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa).
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| IP |
Use at 2-10 µg/mg of lysate.
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| ICC |
1/500 - 1/2000.
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| 特記事項 |
|---|
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WB
1/5000 - 1/15000. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa). |
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IP
Use at 2-10 µg/mg of lysate. |
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ICC
1/500 - 1/2000. |
ターゲット情報
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機能
May be directly involved in regulating the cleavage of polar spindle microtubules and is a key regulator for the onset of cytokinesis during mitosis. Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Phosphorylates 'Ser-10' and 'Ser-28' of histone H3 during mitosis. Required for kinetochore localization of BUB1 and SGOL1. Interacts with INCENP. -
組織特異性
High level expression seen in the thymus. It is also expressed in the spleen, lung, testis, colon, placenta and fetal liver. Expressed during S and G2/M phase and expression is up-regulated in cancer cells during M phase. -
関連疾患
Note=Disruptive regulation of expression is a possibile mechanism of the perturbation of chromosomal integrity in cancer cells through its dominant-negative effect on cytokinesis. -
配列類似性
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily.
Contains 1 protein kinase domain. -
翻訳後修飾
Ubiquitinated by different BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complexes. Ubiquitinated by the BCR(KLHL9-KLHL13) E3 ubiquitin ligase complex, ubiquitination leads to removal from mitotic chromosomes and is required for cytokinesis. During anaphase, the BCR(KLHL21) E3 ubiquitin ligase complex recruits the CPC complex from chromosomes to the spindle midzone and mediates the ubiquitination of AURKB. Ubiquitination of AURKB by BCR(KLHL21) E3 ubiquitin ligase complex may not lead to its degradation by the proteasome. -
細胞内局在
Nucleus. Chromosome. Chromosome > centromere. Cytoplasm > cytoskeleton > spindle. Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Colocalized with gamma tubulin in the mid-body. - Information by UniProt
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参照データベース
- Entrez Gene: 9212 Human
- Omim: 604970 Human
- SwissProt: Q96GD4 Human
- Unigene: 442658 Human
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別名
- AIK2 antibody
- AIM-1 antibody
- AIM1 antibody
see all
画像
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Immunoprecipitation - Anti-Aurora B antibody (ab70238)
Immunoprecipitation of nocodaze-treated HeLa whole cell lysate
Lane 1: ab70238 current lot
Lane 2: ab70238 previous lot
Lane 3: Control IgG
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Western blot - Anti-Aurora B antibody (ab70238)All lanes : Anti-Aurora B antibody (ab70238) at 0.1 µg/ml
Lane 1 : HeLa cells treated with Nocodazole at 50 µg
Lane 2 : HeLa cells mock treated at 50 µg
Lane 3 : HeLa cells treated with Nocodazole at 15 µg
Lane 4 : HeLa cells mock treated at 15 µg
Lane 5 : HeLa cells treated with Nocodazole at 5 µg
Lane 6 : HeLa cells mock treated at 5 µg
Predicted band size: 39 kDa
Exposure time: 3 minutes -
Immunocytochemistry - Anti-Aurora B antibody (ab70238)Immunocytochemistry/Immunofluorescence analysis of asynchronous HeLa cells labelling Aurora B with ab70238 at 0.5µg/ml in A and at 2µg/ml in B. A FITC-conjugated anti-rabbit IgG was used as the secondary antibody in A. A Texas Red-conugated anti-rabbit IgG was used as the secondary antibody in B.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab70238 は 4 報の論文で使用されています。
- Farooq U et al. Polydatin Inhibits Hepatocellular Carcinoma Cell Proliferation and Sensitizes Doxorubicin and Cisplatin through Targeting Cell Mitotic Machinery. Cells 12:N/A (2023). PubMed: 36672157
- Zhu Q et al. Viral-Mediated AURKB Cleavage Promotes Cell Segregation and Tumorigenesis. Cell Rep 26:3657-3671.e5 (2019). PubMed: 30917319
- Martz MK et al. Leukemia-associated RhoGEF (LARG) is a novel RhoGEF in cytokinesis and required for the proper completion of abscission. Mol Biol Cell 24:2785-94 (2013). PubMed: 23885121
- Wang E et al. Aurora B dynamics at centromeres create a diffusion-based phosphorylation gradient. J Cell Biol 194:539-49 (2011). ICC/IF ; Human . PubMed: 21844210