Anti-Aurora A 抗体 [35C1] (ab13824)

Thank you for your reply.

I apologize for the confusion, there was a difference in the numerical order of amino acids listed in the alignment I was performing. You are correct, ab115883 should not be chosen as the immunogen sequence does not overlap with the Chlamy Aurora A sequence.

I reran the search using the proper numerical organization and came up with the following antibody:
ab61114 (https://www.abcam.com/Aurora-A-antibody-ab61114.html): the immunogen sequence is around the human Aurora A amino acid 288. The human and Chlamy sequences are quite homologous in this region, as amino acids 285 and 288 are the only two mismatched amino acids from 275-301.

I'm actually confident enough in the homology within this region to offer our Abtrial program for testing ab61114 in your Chlamy sequences. Information regarding this program is given below:

To our knowledge, ab61114 has not been tested in C. reinhardtii. However by participating in our AbTrial program you can now use our products in an untested application or species without financial risk.

Simply follow these easy steps below to apply for our AbTrial Program:

1. Reply to this email, letting us know you are interested in testing this product.

2. Our scientists will email you an inactive personal discount code for the value of the product.

3. Purchase and test the product at the regular price.

4. Submit your results, including your discount code in the additional notes section of your Abreview.

5. Once the Abreview is submitted, the discount code will become active.

6. Apply your discount code on your next order to receive that value off.

The Terms and Conditions of this offer can be found at: https://www.abcam.com/abtrial

Additionally, until 12/16/12 we are running a rabbit monoclonal antibody promotion, our "RabMab" promotion, which entitles you to a free rabbit monoclonal antibody with purchase of a primary antibody. I found a rabbit monoclonal antibody ab108353 (https://www.abcam.com/Aurora-A-antibody-EPR5026-ab108353.html) that has 54% homology with the Chlamy sequence and might be worth a try, otherwise you could choose a different Rabbit monoclonal antibody that you desire. Details of the RabMab promotion are given below:
https://www.abcam.com/index.html?pageconfig=resource&rid=15447

Please let me know if you have any questions about this offer and I would be happy to help you further.

Thank you for contacting Abcam.

We discussed potential anti-Aurora A antibodies to detect Chlamydomonas reinhardtii Aurora A. Since C. reinhardtii Aurora A has 67.2% homology with human Aurora A, this does not qualify for our AbTrial program, but a positive or negative Abreview can be submitted explaining your experience to give you AbPoints which can be used for an amount off of a future purchase or Amazon gift cards.

I searched through our anti-Aurora A antibodies, and ab115883 is likely your best option should you choose to proceed
https://www.abcam.com/aurora-a-antibody-ab115883.html

The immunogen for this antibody is from amino acids 268-277, which has a fair amount of alignment between human and C. reinhardtii Aurora A.
Human: http://www.uniprot.org/uniprot/O14965
C. reinhardtii: http://www.uniprot.org/uniprot/A8ISU1

Additionally, we currently have a RabMab promotion running until 12/16/12, which entitles you to a Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last. Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

A paper regarding use of the antibody in IP is Cremet JY et al., 2003 which is the last one on the
publication list on our datasheet for ab13824. http://www.ncbi.nlm.nih.gov/pubmed/12619897?dopt=Abstract
This also appears to be the licensors original publication regarding this clone so there might be some extra information in it that will
be helpful.

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

Thank you for your enquiry. I am sorry to hear that you are experiencing difficulties with this product ab13824. Often it is possible to make suggestions that help resolve problems. We will happily offer technical support and in the event that a product is not functioning in the applications cited on the product data sheet (and the problem has been reported within 120 days of purchase), and if it appears that the antibody is at fault, a replacement/credit note/refund will be offered. I have looked at the protocols you used and have a few questions and suggestions that might help you determine the cause of the no staining results. I would therefore appreciate if you can please clarify the following items. Since you are using mouse cell samples, then fixation in 4% PFA should be done not more than 15 minutes, otherwise your epitopes will be masked. Also, we have a customer who previous used this product and mentions that this antibody only recognizes a centrosome associated epitope in methanol-fixed cells and does not recognize anything in a paraformaldehyde-fixed cell. Please see the abreview by Kirk McManus submitted on 19 April 2007 on the datasheet. Can you confirm that the second antibody also managed to produce good results with other primary antibodies? In some cases, the problem may be with the secondary antibody not working properly. I hope the above recommendations may already help you. If you have already tried the above suggestions and still experience problems, please do not hesitate to contact me with details of your order (purchase order number, batch number, shipping address/purchasing agent information). Also please advice me on how you would like to proceed with your enquiry, so that I can immediately arrange for a replacement or refund to you.