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リコンビナントRabMAb

Anti-AUH 抗体 [EPR11086(B)] (ab157453)

Submit a review Submit a question References (3)
Western blot - Anti-AUH antibody [EPR11086(B)] (ab157453)
  • Immunocytochemistry/ Immunofluorescence - Anti-AUH antibody [EPR11086(B)] (ab157453)
  • Anti-AUH antibody [EPR11086(B)] (ab157453)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11086(B)] to AUH
  • Suitable for: WB, ICC/IF
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-AUH antibody [EPR11086(B)]
    AUH 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR11086(B)] to AUH

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, ICC/IFmore details
    適用なし: IHC-P or IP

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • Mouse brain, Rat brain, Rat heart and Rat kidney cell lysates; SH-SY5Y cells.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab157453の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/1000 - 1/10000. Predicted molecular weight: 36 kDa.
ICC/IF
1/100 - 1/250.
特記事項
WB
1/1000 - 1/10000. Predicted molecular weight: 36 kDa.
ICC/IF
1/100 - 1/250.
追加情報
Is unsuitable for IHC-P or IP.

ターゲット情報

  • 関連性

    AUH (3-methylglutaconyl-CoA hydratase) catalyzes the conversion of 3-methylglutaconyl-CoA to 3-hydroxy-3-methylglutaryl-CoA and has very low enoyl-CoA hydratase activity. Deletion or mutation of the AUH gene causes the metabolic disease 3-methylglutaconic aciduria type I (MGA1). MGA type I is characterized by an abnormal organic acid profile in which there is excessive urinary excretion of 3-methylglutaconic acid, 3-methylglutaric acid and 3-hydroxyisovaleric acid. AUH is also an RNA-binding protein that binds in vitro to clustered 5'-AUUUA-3' motifs.

  • 細胞内局在

    Mitochondrial

  • 参照データベース

    • 別名

      • 3 methylglutaconyl CoA hydratase antibody
      • AU binding protein/enoyl CoA hydratase antibody
      • AU RNA binding protein/enoyl CoA hydratase antibody
      • AU RNA binding protein/enoyl Coenzyme A hydratase antibody
      • AU specific RNA binding antibody
      • AU specific RNA binding enoyl CoA hydratase antibody
      • Methylglutaconyl CoA hydratase antibody
      • Methylglutaconyl CoA hydratase mitochondrial antibody
      • MGA Type I antibody
      see all

    画像

    • Western blot - Anti-AUH antibody [EPR11086(B)] (ab157453)
      Western blot - Anti-AUH antibody [EPR11086(B)] (ab157453)
      All lanes : Anti-AUH antibody [EPR11086(B)] (ab157453) at 1/1000 dilution

      Lane 1 : Mouse brain cell lysate
      Lane 2 : Rat brain cell lysate
      Lane 3 : Rat heart cell lysate
      Lane 4 : Rat kidney cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 36 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-AUH antibody [EPR11086(B)] (ab157453)
      Immunocytochemistry/ Immunofluorescence - Anti-AUH antibody [EPR11086(B)] (ab157453)
      Immunofluorescent analysis of SH-SY5Y cells labeling AUH with ab157453 at 1/100 dilution.
    • Anti-AUH antibody [EPR11086(B)] (ab157453)
      Anti-AUH antibody [EPR11086(B)] (ab157453)

    参考文献 (3)

    ab157453 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab157453 は 3 報の論文で使用されています。

    • Son SM  et al. Leucine regulates autophagy via acetylation of the mTORC1 component raptor. Nat Commun 11:3148 (2020). PubMed: 32561715
    • Son SM  et al. Leucine Signals to mTORC1 via Its Metabolite Acetyl-Coenzyme A. Cell Metab 29:192-201.e7 (2019). PubMed: 30197302
    • Zhang Y  et al. PINK1 Inhibits Local Protein Synthesis to Limit Transmission of Deleterious Mitochondrial DNA Mutations. Mol Cell 73:1127-1137.e5 (2019). PubMed: 30772175

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