Anti-ATR 抗体 (ab2905)
Key features and details
- Rabbit polyclonal to ATR
- Suitable for: ChIP, WB, ICC/IF, IP
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-ATR antibody
ATR 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to ATR -
由来種
Rabbit -
アプリケーション
適用あり: ChIP, WB, ICC/IF, IPmore details -
種交差性
交差種: Mouse, Human -
免疫原
Fusion protein, corresponding to amino acids 400-460 of Human ATR.
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ポジティブ･コントロール
- WB: K562 lysate
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.05% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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ChIP Related Products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab2905の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ChIP |
Use at an assay dependent concentration. PubMed: 20647539
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| WB | (17) |
1/1000. Detects a band of approximately 305 kDa (predicted molecular weight: 309 kDa). (in lysate from UV irradiated K562 cells).
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| ICC/IF | (3) |
Use at an assay dependent concentration.
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| IP | (2) |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
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ChIP
Use at an assay dependent concentration. PubMed: 20647539 |
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WB
1/1000. Detects a band of approximately 305 kDa (predicted molecular weight: 309 kDa). (in lysate from UV irradiated K562 cells). |
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ICC/IF
Use at an assay dependent concentration. |
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IP
Use at an assay dependent concentration. |
ターゲット情報
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機能
Serine/threonine protein kinase which activates checkpoint signaling upon genotoxic stresses such as ionizing radiation (IR), ultraviolet light (UV), or DNA replication stalling, thereby acting as a DNA damage sensor. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates BRCA1, CHEK1, MCM2, RAD17, RPA2, SMC1 and p53/TP53, which collectively inhibit DNA replication and mitosis and promote DNA repair, recombination and apoptosis. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX at sites of DNA damage, thereby regulating DNA damage response mechanism. Required for FANCD2 ubiquitination. Critical for maintenance of fragile site stability and efficient regulation of centrosome duplication. -
組織特異性
Ubiquitous, with highest expression in testis. Isoform 2 is found in pancreas, placenta and liver but not in heart, testis and ovary. -
関連疾患
Defects in ATR are a cause of Seckel syndrome type 1 (SCKL1) [MIM:210600]. SCKL1 is a rare autosomal recessive disorder characterized by growth retardation, microcephaly with mental retardation, and a characteristic 'bird-headed' facial appearance. -
配列類似性
Belongs to the PI3/PI4-kinase family. ATM subfamily.
Contains 1 FAT domain.
Contains 1 FATC domain.
Contains 2 HEAT repeats.
Contains 1 PI3K/PI4K domain. -
翻訳後修飾
Phosphorylated; autophosphorylates in vitro. -
細胞内局在
Nucleus. Nucleus > PML body. Depending on the cell type, it can also be found in PML nuclear bodies. Recruited to chromatin during S-phase. Redistributes to discrete nuclear foci upon DNA damage, hypoxia or replication fork stalling. - Information by UniProt
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参照データベース
- Entrez Gene: 545 Human
- Entrez Gene: 245000 Mouse
- Omim: 601215 Human
- SwissProt: Q13535 Human
- SwissProt: Q9JKK8 Mouse
- Unigene: 271791 Human
- Unigene: 212462 Mouse
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別名
- Ataxia telangiectasia and Rad3 related antibody
- Ataxia telangiectasia and Rad3-related protein antibody
- ATR antibody
see all
画像
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Western blot - Anti-ATR antibody (ab2905)Western blot on ATR in K562 whole cell lysate using ab2905.
データシートおよび資料
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Datasheet download
参考文献 (47)
ab2905 は 47 報の論文で使用されています。
- Qi F et al. The ribonuclease domain function is dispensable for SLFN11 to mediate cell fate decision during replication stress response. Genes Cells 28:663-673 (2023). PubMed: 37469008
- Zhang C et al. BMAL1 collaborates with CLOCK to directly promote DNA double-strand break repair and tumor chemoresistance. Oncogene 42:967-979 (2023). PubMed: 36725890
- Nelson TJ & Xu Y Sting and p53 DNA repair pathways are compromised in Alzheimer's disease. Sci Rep 13:8304 (2023). PubMed: 37221295
- Heyza JR et al. ATR inhibition overcomes platinum tolerance associated with ERCC1- and p53-deficiency by inducing replication catastrophe. NAR Cancer 5:zcac045 (2023). PubMed: 36644397
- Niwa Y et al. Cyclin D1 Binding Protein 1 Responds to DNA Damage through the ATM-CHK2 Pathway. J Clin Med 11:N/A (2022). PubMed: 35160302
