AB10312

Anti-ATR 抗体

Name: Anti-ATR 抗体 (ab10312) | Abcam
Brand: Abcam Limited
SKU: AB10312
Price: 78000 JPY
Availability: InStock

Anti-ATR antibody

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(13 Publications)

Rabbit Polyclonal ATR antibody. Suitable for WB and reacts with Human samples. Cited in 13 publications. Immunogen corresponding to Synthetic Peptide within Human ATR aa 400-450.

別名を表示する

FRP1, ATR, Serine/threonine-protein kinase ATR, Ataxia telangiectasia and Rad3-related protein, FRAP-related protein 1

2 Images

Unknown

Western blot - Anti-ATR antibody (AB10312)

Detection of human ATR by Western Blot. Samples : Whole cell lysate (20 μg) from HeLa and U2OS cells separated on a 3 to 8% tris-acetate gel. Antibody : ab10312 used at 0.07 mg/ml. Detection : Chemiluminescence with 15 second exposure. Detection of human ATR by Western Blot. Samples : Whole cell lysate (20 µg) from HeLa and U2OS cells separated on a 3 to 8% tris-acetate gel. Antibody : ab10312 used at 0.07 mg/ml. Detection : Chemiluminescence with 15 second exposure.

All lanes:

Western blot - Anti-ATR antibody (ab10312)

Predicted band size: 301 kDa

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Lab

Western blot - Anti-ATR antibody (AB10312)

Western blot : Anti-ATR antibody staining at 1/15000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red.
In Western blot, ab10312 was shown to bind specifically to ATR. A band was observed at 260 kDa in wild-type A549 cell lysates with a reduction in signal observed at this size in ATR heterozygous knockout cell line ab276104 (knockout cell lysate ab277987). To generate this image, wild-type and ATR heterozygous knockout A549 cell lysates were analysed.
Nitrocellulose membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again then imaged.

Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.

All lanes:

Western blot - Anti-ATR antibody (ab10312) at 1/15000 dilution

Lane 1:

Wild-type A549 cell lysate at 20 µg

Lane 2:

ATR knockout A549 cell lysate at 20 µg

Lane 2:

Western blot - Human ATR knockout (hetero) A549 cell line (<a href='/products/cell-lines/human-atr-knockout-hetero-a549-cell-line-ab276104'>ab276104</a>)

Lane 3:

HeLa cell lysate at 20 µg

Predicted band size: 301 kDa

Observed band size: 260 kDa

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Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

applications

免疫原

Synthetic Peptide within Human ATR aa 400-450. The exact immunogen used to generate this antibody is proprietary information.

Q13535

特異性

We have data to indicate that this antibody may not cross react with Xenopus laevis. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/15000 - 1/30000", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

バッファー組成

pH: 7 - 8 Preservative: 0.1% Sodium azide Constituents: PBS, 1.815% Tris, 1.764% Sodium citrate

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

ATR also known as Ataxia Telangiectasia and Rad3-related protein is a serine/threonine kinase with a molecular weight of approximately 301 kDa. This protein localizes mainly in the nucleus where it functions as an important component in the cellular response to DNA damage and replication stress. ATR detects DNA strand breaks and ssDNA coated with RPA and becomes activated to phosphorylate several downstream targets initiating the DNA damage response. High expression of ATR occurs in proliferative tissues emphasizing its role in cell cycle regulation.

Biological function summary

ATR plays an essential role in maintaining genomic stability. It is part of a larger protein complex that includes ATRIP (ATR-interacting protein) which helps in localizing ATR to sites of DNA damage. Once activated ATR phosphorylates various substrates including CHK1 a critical checkpoint kinase involved in cell cycle arrest during DNA repair processes. The ability of ATR to coordinate with these proteins helps cells manage DNA damage effectively and prevent genomic instability.

Pathways

ATR functions centrally in the DNA damage response and repair mechanisms particularly the ATR-Chk1 pathway. This pathway interacts closely with the ATM (Ataxia Telangiectasia Mutated) pathway which also responds to DNA damage but usually to double-strand breaks. ATR primarily acts in response to replication stress and its activation leads to the arrest of the cell cycle allowing DNA repair to occur. This cooperation between ATR and ATM highlights their complementary roles in safeguarding genomic integrity under stress.

ATR mutations and dysregulation have strong associations with cancer and Seckel syndrome. In the context of cancer ATR often works in concert with ATM to manage DNA repair and cancer cells frequently overexpress ATR to cope with high levels of replication stress. This makes ATR a potential target for cancer therapy where its inhibition could sensitize tumor cells to chemotherapy. In Seckel syndrome ATR mutations result in developmental anomalies showcasing the important role ATR plays in cellular replication and repair processes.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Serine/threonine protein kinase which activates checkpoint signaling upon genotoxic stresses such as ionizing radiation (IR), ultraviolet light (UV), or DNA replication stalling, thereby acting as a DNA damage sensor (PubMed : 10597277, PubMed : 10608806, PubMed : 10859164, PubMed : 11721054, PubMed : 12791985, PubMed : 12814551, PubMed : 14657349, PubMed : 14729973, PubMed : 14742437, PubMed : 15210935, PubMed : 15496423, PubMed : 16260606, PubMed : 21144835, PubMed : 21777809, PubMed : 23273981, PubMed : 25083873, PubMed : 27723717, PubMed : 27723720, PubMed : 30139873, PubMed : 33848395, PubMed : 37788673, PubMed : 37832547, PubMed : 9427750, PubMed : 9636169). Recognizes the substrate consensus sequence [ST]-Q (PubMed : 10597277, PubMed : 10608806, PubMed : 10859164, PubMed : 11721054, PubMed : 12791985, PubMed : 12814551, PubMed : 14657349, PubMed : 14729973, PubMed : 14742437, PubMed : 15210935, PubMed : 15496423, PubMed : 16260606, PubMed : 21144835, PubMed : 23273981, PubMed : 27723717, PubMed : 27723720, PubMed : 33848395, PubMed : 9427750, PubMed : 9636169). Phosphorylates BRCA1, CHEK1, MCM2, RAD17, RBBP8, RPA2, SMC1 and p53/TP53, which collectively inhibit DNA replication and mitosis and promote DNA repair, recombination and apoptosis (PubMed : 11114888, PubMed : 11418864, PubMed : 11865061, PubMed : 21777809, PubMed : 23273981, PubMed : 25083873, PubMed : 9925639). Phosphorylates 'Ser-139' of histone variant H2AX at sites of DNA damage, thereby regulating DNA damage response mechanism (PubMed : 11673449). Required for FANCD2 ubiquitination (PubMed : 15314022). Critical for maintenance of fragile site stability and efficient regulation of centrosome duplication (PubMed : 12526805). Acts as a regulator of the S-G2 transition by restricting the activity of CDK1 during S-phase to prevent premature entry into G2 (PubMed : 30139873). Acts as a regulator of the nuclear envelope integrity in response to DNA damage and stress (PubMed : 25083873, PubMed : 37788673, PubMed : 37832547). Acts as a mechanical stress sensor at the nuclear envelope : relocalizes to the nuclear envelope in response to mechanical stress and mediates a checkpoint via phosphorylation of CHEK1 (PubMed : 25083873). Also promotes nuclear envelope rupture in response to DNA damage by mediating phosphorylation of LMNA at 'Ser-282', leading to lamin disassembly (PubMed : 37832547). Involved in the inflammatory response to genome instability and double-stranded DNA breaks : acts by localizing to micronuclei arising from genome instability and catalyzing phosphorylation of LMNA at 'Ser-395', priming LMNA for subsequent phosphorylation by CDK1 and micronuclei envelope rupture (PubMed : 37788673). The rupture of micronuclear envelope triggers the cGAS-STING pathway thereby activating the type I interferon response and innate immunity (PubMed : 37788673). Positively regulates the restart of stalled replication forks following activation by the KHDC3L-OOEP scaffold complex (By similarity).

See full target information ATR

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文献 (13)

Recent publications for all applications. Explore the full list and refine your search

British journal of cancer 133:381-393 PubMed40442403

2025

Sequential ATR and PARP inhibition overcomes acquired DNA damaging agent resistance in pancreatic ductal adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Katharine J Herbert,Rosie Upstill-Goddard,Stephan B Dreyer,Selma Rebus,Christian Pilarsky,Mukhopadhyay Debabrata,Christopher J Lord,Andrew V Biankin,Fieke E M Froeling,David K Chang

NPJ precision oncology 9:15 PubMed39809873

2025

A targetable OSGIN1 - AMPK - SLC2A3 axis controls the vulnerability of ovarian cancer to ferroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Mengqi Deng,Fan Tang,Xiangyu Chang,Yanqin Zhang,Penglin Liu,Xuechao Ji,Yubo Zhang,Ruiye Yang,Junyi Jiang,Junqi He,Jinwei Miao

Applied microbiology and biotechnology 109:1 PubMed39747723

2025

Targeted knockdown of ATM, ATR, and PDEδ increases Gag HIV-1 VLP production in HEK293 cells.

Applications

Unspecified application

Species

Unspecified reactive species

Andy Díaz-Maneh,Pol Pérez-Rubio,Cristina Rigau Granes,Laia Bosch-Molist,Jesús Lavado-García,Francesc Gòdia,Laura Cervera

JCI insight 9: PubMed39499573

2024

LMAN1 serves as a cargo receptor for thrombopoietin.

Applications

Unspecified application

Species

Unspecified reactive species

Lesley A Everett,Zesen Lin,Ann Friedman,Vi T Tang,Greggory Myers,Ginette Balbin-Cuesta,Richard King,Guojing Zhu,Beth McGee,Rami Khoriaty

Cell reports 31:107723 PubMed32492431

2020

Cas12a Base Editors Induce Efficient and Specific Editing with Low DNA Damage Response.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao Wang,Chengfeng Ding,Wenxia Yu,Ying Wang,Siting He,Bei Yang,Yi-Chun Xiong,Jia Wei,Jifang Li,Jiayi Liang,Zongyang Lu,Wei Zhu,Jing Wu,Zhi Zhou,Xingxu Huang,Zhen Liu,Li Yang,Jia Chen

The Journal of investigative dermatology 139:2016-2028.e7 PubMed30878676

2019

Loss of Epidermal HIF-1α Blocks UVB-Induced Tumorigenesis by Affecting DNA Repair Capacity and Oxidative Stress.

Applications

Unspecified application

Species

Unspecified reactive species

Walid Mahfouf,Mohsen Hosseini,Elodie Muzotte,Martin Serrano-Sanchez,Lea Dousset,François Moisan,Walid Rachidi,Alain Taieb,Jana Rudolf,Hamid Reza Rezvani

Nature communications 10:910 PubMed30796221

2019

Overexpression of Claspin and Timeless protects cancer cells from replication stress in a checkpoint-independent manner.

Applications

Unspecified application

Species

Unspecified reactive species

Julien N Bianco,Valérie Bergoglio,Yea-Lih Lin,Marie-Jeanne Pillaire,Anne-Lyne Schmitz,Julia Gilhodes,Amelie Lusque,Julien Mazières,Magali Lacroix-Triki,Theodoros I Roumeliotis,Jyoti Choudhary,Jérôme Moreaux,Jean-Sébastien Hoffmann,Hélène Tourrière,Philippe Pasero

Oncology reports 39:1485-1493 PubMed29344638

2018

Garcinone C exerts antitumor activity by modulating the expression of ATR/Stat3/4E‑BP1 in nasopharyngeal carcinoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yudui Xia,Xia Liu,Chunlin Zou,Shixiu Feng,Hongwei Guo,Yeguo Yang,Yong Lei,Jian Zhang,Yi Lu

Nature cell biology 16:864-75 PubMed25086746

2014

ATM-mediated stabilization of ZEB1 promotes DNA damage response and radioresistance through CHK1.

Applications

Unspecified application

Species

Unspecified reactive species

Peijing Zhang,Yongkun Wei,Li Wang,Bisrat G Debeb,Yuan Yuan,Jinsong Zhang,Jingsong Yuan,Min Wang,Dahu Chen,Yutong Sun,Wendy A Woodward,Yongqing Liu,Douglas C Dean,Han Liang,Ye Hu,K Kian Ang,Mien-Chie Hung,Junjie Chen,Li Ma

eLife 3:e02001 PubMed24843013

2014

Repair of naturally occurring mismatches can induce mutations in flanking DNA.

Applications

Unspecified application

Species

Unspecified reactive species

Jia Chen,Brendan F Miller,Anthony V Furano

View all publications

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Abcam product promise

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Anti-ATR 抗体

Anti-ATR antibody

Western blot - Anti-ATR antibody (AB10312)

Western blot - Anti-ATR antibody (AB10312)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

特異性

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (13)

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Abcam product promise