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Anti-ATPB 抗体 [10/ATP] - BSA and Azide free (ab289897)

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Immunocytochemistry/ Immunofluorescence - Anti-ATPB antibody [10/ATP] - BSA and Azide free (ab289897)
  • Western blot - Anti-ATPB antibody [10/ATP] - BSA and Azide free (ab289897)
  • Immunocytochemistry/ Immunofluorescence - Anti-ATPB antibody [10/ATP] - BSA and Azide free (ab289897)
  • Immunocytochemistry/ Immunofluorescence - Anti-ATPB antibody [10/ATP] - BSA and Azide free (ab289897)
  • Anti-ATPB antibody [10/ATP] - BSA and Azide free (ab289897)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [10/ATP] to ATPB - BSA and Azide free
  • Suitable for: ICC/IF, WB
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Unconjugated

製品の概要

  • 製品名

    Anti-ATPB antibody [10/ATP] - BSA and Azide free
    ATPB 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [10/ATP] to ATPB - BSA and Azide free

  • 由来種

    Mouse

  • アプリケーション

    適用あり: ICC/IF, WBmore details

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Jurkat, HeLa, A549, MCF7, RAW264.7, NIH/3T3, C6 and PC-12 whole cell lysates. ICC/IF: A549, RAW 264.7 and PC-12 cells.

  • 特記事項

    ab289897 is a carrier-free version of ab289890. 

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab289897の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ICC/IF
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 52 kDa (predicted molecular weight: 56 kDa).
特記事項
ICC/IF
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 52 kDa (predicted molecular weight: 56 kDa).

ターゲット情報

  • 機能

    Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F(1). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits.

  • 配列類似性

    Belongs to the ATPase alpha/beta chains family.

  • 細胞内局在

    Mitochondrion. Mitochondrion inner membrane. Peripheral membrane protein.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • ATP 5B antibody
    • ATP synthase H+ transporting mitochondrial F1 complex beta polypeptide antibody
    • ATP synthase subunit beta mitochondrial antibody
    • ATP synthase subunit beta, mitochondrial antibody
    • atp5b antibody
    • ATPB antibody
    • ATPB_HUMAN antibody
    • ATPMB antibody
    • ATPSB antibody
    • Epididymis secretory protein Li 271 antibody
    • HEL-S-271 antibody
    • Mitochondrial ATP synthase beta subunit antibody
    • Mitochondrial ATP Synthase Subunit Beta antibody
    • Mitochondrial ATP synthetase beta subunit antibody
    see all

画像

  • Immunocytochemistry/ Immunofluorescence - Anti-ATPB antibody [10/ATP] - BSA and Azide free (ab289897)
    Immunocytochemistry/ Immunofluorescence - Anti-ATPB antibody [10/ATP] - BSA and Azide free (ab289897)

    This data was developed using ab289890, the same antibody clone in a different buffer formulation. 

    Immunofluorescent analysis of 100% methanol-fixed, 0.1% Triton X-100 permeabilized A549（human lung carcinoma epithelial cell line) cells labeling ATPB with ab289890 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150117) secondary antibody at 1/1000 dilution (green). Confocal image showing mitochondria staining in A549 cell line. The nuclear counter stain is DAPI (blue).

    ab186735 Anti-TOMM20 Rabbit monoclonal antibody - Mitochondrial Marker was used at 1/500 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150080) secondary antibody at 1/500 dilution (red).

    The negative controls are as follows:
    -ve control 1: ab289890 at 1/100 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150080) secondary antibody at 1/500 dilution.
    -ve control 2: ab186735 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150117) secondary antibody at 1/1000 dilution.

  • Western blot - Anti-ATPB antibody [10/ATP] - BSA and Azide free (ab289897)
    Western blot - Anti-ATPB antibody [10/ATP] - BSA and Azide free (ab289897)
    All lanes : Anti-ATPB antibody [10/ATP] (ab289890) at 1/1000 dilution

    Lane 1 : Jurkat (human T cell leukemia T lymphocyte), whole cell lysate
    Lane 2 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
    Lane 3 : A549 (human lung carcinoma epithelial cell), whole cell lysate
    Lane 4 : MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate
    Lane 5 : RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate
    Lane 6 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate
    Lane 7 : C6 (rat glial tumor glial cell), whole cell lysate
    Lane 8 : PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/100000 dilution

    Predicted band size: 56 kDa
    Observed band size: 52 kDa why is the actual band size different from the predicted?



    This data was developed using ab289890, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST. 

    Eposure times: 

    Lanes 1-6: 6 seconds
    Lanes 7-8: 114 seconds

     

  • Immunocytochemistry/ Immunofluorescence - Anti-ATPB antibody [10/ATP] - BSA and Azide free (ab289897)
    Immunocytochemistry/ Immunofluorescence - Anti-ATPB antibody [10/ATP] - BSA and Azide free (ab289897)

    This data was developed using ab289890, the same antibody clone in a different buffer formulation. 

    Immunofluorescent analysis of 100% methanol-fixed, 0.1% Triton X-100 permeabilized RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage cell line) cells labeling ATPB with ab289890 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150117) secondary antibody at 1/1000 dilution (green). Confocal image showing mitochondrial staining in RAW 264.7 cell line. The nuclear counter stain is DAPI (blue).

    ab186735 Anti-TOMM20 Rabbit monoclonal antibody - Mitochondrial Marker was used at 1/500 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150080) secondary antibody at 1/500 dilution (red).

    The negative controls are as follows:
    -ve control 1: ab289890 at 1/100 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150080) secondary antibody at 1/500 dilution.
    -ve control 2: ab186735 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150117) secondary antibody at 1/1000 dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-ATPB antibody [10/ATP] - BSA and Azide free (ab289897)
    Immunocytochemistry/ Immunofluorescence - Anti-ATPB antibody [10/ATP] - BSA and Azide free (ab289897)

    This data was developed using ab289890, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 80% methanol-fixed, 0.1% Triton X-100 permeabilized PC-12 (rat adrenal gland pheochromocytoma cell line) cells labeling ATPB with ab289890 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150117) secondary antibody at 1/1000 dilution (green). Confocal image showing mitochondria staining in RAW PC-12 cell line. The nuclear counter stain is DAPI (blue).

    ab186735 Anti-TOMM20 Rabbit monoclonal antibody - Mitochondrial Marker was used at 1/500 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150080) secondary antibody at 1/500 dilution (red).

    The negative controls are as follows:
    -ve control 1: ab289890 at 1/100 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150080) secondary antibody at 1/500 dilution.
    -ve control 2: ab186735 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150117) secondary antibody at 1/1000 dilution. 

  • Anti-ATPB antibody [10/ATP] - BSA and Azide free (ab289897)
    Anti-ATPB antibody [10/ATP] - BSA and Azide free (ab289897)

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (0)

ab289897 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab289897 は論文での使用が確認できていません。

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