Anti-ATP6V1B2 抗体 (ab183887)
Key features and details
- Rabbit polyclonal to ATP6V1B2
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-ATP6V1B2 antibody
ATP6V1B2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to ATP6V1B2 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WBmore details -
種交差性
交差種: Mouse, Rat, Human
交差が予測される動物種: Cow
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免疫原
Recombinant fragment within Human ATP6V1B2 aa 37-320. The exact sequence is proprietary.
Database link: P21281 -
ポジティブ･コントロール
- U87-MG, SK-N-SH, IMR32 and SK-N-AS whole cell lysate/extract. Mouse and rat brain lysate/extract. Mouse kidney tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab183887の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
1/100 - 1/1000.
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| WB |
1/500 - 1/10000. Predicted molecular weight: 57 kDa.
|
| 特記事項 |
|---|
|
IHC-P
1/100 - 1/1000. |
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WB
1/500 - 1/10000. Predicted molecular weight: 57 kDa. |
ターゲット情報
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機能
Non-catalytic subunit of the peripheral V1 complex of vacuolar ATPase. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells. -
配列類似性
Belongs to the ATPase alpha/beta chains family. -
細胞内局在
Endomembrane system. Melanosome. Endomembrane. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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参照データベース
- Entrez Gene: 338082 Cow
- Entrez Gene: 526 Human
- Entrez Gene: 11966 Mouse
- Entrez Gene: 117596 Rat
- Omim: 606939 Human
- SwissProt: P31408 Cow
- SwissProt: P21281 Human
- SwissProt: P62814 Mouse
see all -
別名
- ATP6B1B2 antibody
- ATP6B2 antibody
- ATP6V1 B2 antibody
see all
画像
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Western blot - Anti-ATP6V1B2 antibody (ab183887)All lanes : Anti-ATP6V1B2 antibody (ab183887) at 1/1000 dilution
Lane 1 : U87-MG whole cell lysate
Lane 2 : SK-N-SH whole cell lysate
Lane 3 : IMR32 whole cell lysate
Lane 4 : SK-N-AS whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 57 kDa7.5% SDS PAGE
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP6V1B2 antibody (ab183887)Immunohistochemical analysis of paraffin embedded mouse kidney labeling ATP6V1B2 with ab183887 at 1/500 dilution, showing cytosol staining.
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Western blot - Anti-ATP6V1B2 antibody (ab183887)Anti-ATP6V1B2 antibody (ab183887) at 1/5000 dilution + Rat brain at 50 µg
Predicted band size: 57 kDa7.5% SDS PAGE
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Western blot - Anti-ATP6V1B2 antibody (ab183887)Anti-ATP6V1B2 antibody (ab183887) at 1/5000 dilution + Mouse brain lysate at 50 µg
Predicted band size: 57 kDa7.5% SDS PAGE
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab183887 は 1 報の論文で使用されています。
- Kontaxi C et al. Epilepsy-Related CDKL5 Deficiency Slows Synaptic Vesicle Endocytosis in Central Nerve Terminals. J Neurosci 43:2002-2020 (2023). PubMed: 36759195