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AB113745

Anti-ATP6V1A 抗体 [8B1F3]

Anti-ATP6V1A antibody [8B1F3]

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(2 Publications)

Mouse Monoclonal ATP6V1A antibody. Suitable for WB and reacts with Saccharomyces cerevisiae samples. Cited in 2 publications.

別名を表示する

CLS8, TFP1, YDL185W, D1286, VMA1, V-type proton ATPase catalytic subunit A, V-ATPase subunit A, Vacuolar proton pump subunit A

1 Images
Western blot - Anti-ATP6V1A antibody [8B1F3] (AB113745)
  • WB

Unknown

Western blot - Anti-ATP6V1A antibody [8B1F3] (AB113745)

All lanes:

Western blot - Anti-ATP6V1A antibody [8B1F3] (ab113745) at 1 µg/mL

All lanes:

Yeast Whole Cell at 15 µg

Predicted band size: 68 kDa

false

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

8B1F3

アイソタイプ

IgG2a

軽鎖のタイプ

kappa

キャリアフリー

No

交差種

Saccharomyces cerevisiae

アプリケーション

WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Saccharomyces cerevisiae": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-1 µg/mL", "WB-species-notes": "<p></p>" } } }
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製品の詳細

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Immunogen
精製に関する特記事項
Near homogeneity. The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by chemical fractionation.
バッファー組成
pH: 7.5 Preservative: 0.02% Sodium azide Constituents: HEPES buffered saline
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
+4°C
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

ATP6V1A also known as V-ATPase subunit A is a component of the V1 domain of the vacuolar ATPase (V-ATPase) which is a large enzyme responsible for acidifying intracellular compartments. This protein has a mass of approximately 70 kDa and operates in cellular processes by hydrolyzing ATP to drive protons across membranes. ATP6V1A displays significant expression in several cell types including neurons epithelial cells and osteoclasts. The protein enables V-ATPase functionality important for maintaining the pH balance inside cells and organelles facilitating various physiological processes.
Biological function summary

ATP6V1A is an integral part of the V1 domain of the V-ATPase complex a multi-subunit assembly critical for the acidification of intracellular organelles such as lysosomes endosomes and the Golgi apparatus. This activity supports processes like protein degradation receptor-mediated endocytosis and membrane trafficking. The V-ATPase containing ATP6V1A uses energy from ATP hydrolysis to pump protons which is essential for cellular homeostasis and ion transport.

Pathways

ATP6V1A plays an important role in the endocytosis and autophagy pathways. These pathways depend on the acidification of intracellular compartments enabling processes such as the breakdown and recycling of cellular components. ATP6V1A is related to proteins like V-ATPase subunit c (ATP6V1C) and other subunits that form the V-ATPase enzyme. These protein interactions allow the modulation and execution of cellular transport and hormone regulation pathways.

Mutations or dysregulation of ATP6V1A have been linked to renal tubular acidosis and osteoporosis where improper acidification impacts bone resorption and kidney function. The protein connections include its interaction with other V-ATPase subunits like ATP6V0A3 which can also result in osteopetrosis when defective. Such conditions demonstrate how ATP6V1A's role in acidification processes is important for normal physiological function and how its dysfunction leads to disease.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Catalytic subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons (PubMed : 18055462, PubMed : 2139027). V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments (PubMed : 18055462, PubMed : 2139027).. PI-SceI is an endonuclease that can cleave at a site present in a VMA1 allele that lacks the derived endonuclease segment of the open reading frame; cleavage at this site only occurs during meiosis and initiates 'homing', a genetic event that converts a VMA1 allele lacking VDE into one that contains it.
See full target information VMA1
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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of cell science 130:2317-2328 PubMed28576969

2017

Redox regulation of the yeast voltage-gated Ca channel homolog Cch1p by glutathionylation of specific cysteine residues.

Applications

Unspecified application

Species

Unspecified reactive species

Avinash Chandel,Anand K Bachhawat

Molecular biology of the cell 27:3913-3925 PubMed27708136

2016

Glutathione depletion activates the yeast vacuolar transient receptor potential channel, Yvc1p, by reversible glutathionylation of specific cysteines.

Applications

IF

Species

Unspecified reactive species

Avinash Chandel,Krishna K Das,Anand K Bachhawat
View all publications
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