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Anti-ATP6V0D1/P39 抗体 [2G12] (ab56441)

Reviews (1)Q&A (1)References (19)
Western blot - Anti-ATP6V0D1/P39 antibody [2G12] (ab56441)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP6V0D1/P39 antibody [2G12] (ab56441)
  • Immunocytochemistry/ Immunofluorescence - Anti-ATP6V0D1/P39 antibody [2G12] (ab56441)
  • Flow Cytometry - Anti-ATP6V0D1/P39 antibody [2G12] (ab56441)

Key features and details

  • Mouse monoclonal [2G12] to ATP6V0D1/P39
  • Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
  • Reacts with: Human
  • Isotype: IgG1

製品の概要

  • 製品名

    Anti-ATP6V0D1/P39 antibody [2G12]
    ATP6V0D1/P39 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [2G12] to ATP6V0D1/P39

  • 由来種

    Mouse

  • アプリケーション

    適用あり: WB, IHC-P, ICC/IF, Flow Cytmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Recombinant fragment corresponding to Human ATP6V0D1/P39 aa 200-350.
    Database link: P61421

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • 特記事項

    This product was changed from ascites to tissue culture supernatant on 13th Feb 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

  • バッファー

    pH: 7.4
  • Concentration information loading...

  • 精製度

    Tissue culture supernatant

  • ポリ/モノ

    モノクローナル

  • クローン名

    2G12

  • アイソタイプ

    IgG1

  • 軽鎖の種類

    kappa

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab56441の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB (1)
Use at an assay dependent concentration. Predicted molecular weight: 40 kDa.
IHC-P
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
特記事項
WB
Use at an assay dependent concentration. Predicted molecular weight: 40 kDa.
IHC-P
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能

    Subunit of the integral membrane V0 complex of vacuolar ATPase. Vacuolar ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells, thus providing most of the energy required for transport processes in the vacuolar system. May play a role in coupling of proton transport and ATP hydrolysis (By similarity). May play a role in cilium biogenesis through regulation of the transport and the localization of proteins to the cilium.

  • 組織特異性

    Ubiquitous.

  • 配列類似性

    Belongs to the V-ATPase V0D/AC39 subunit family.

  • 細胞内局在

    Membrane. Localizes to centrosome and the base of the cilium.
  • Information by UniProt

  • 参照データベース

    • 別名

      • 32 kDa accessory protein antibody
      • ATP6D antibody
      • ATP6DV antibody
      • ATP6V0D1 antibody
      • ATPase H+ transporting lysosomal (vacuolar proton pump) member D antibody
      • ATPase H+ transporting lysosomal 38kD V0 subunit d antibody
      • ATPase H+ transporting lysosomal 38kDa V0 subunit d1 antibody
      • ATPase H+ transporting lysosomal V0 subunit d1 antibody
      • H(+) transporting two sector ATPase subunit D antibody
      • p39 antibody
      • V ATPase 40 KDa accessory protein antibody
      • V ATPase AC39 subunit antibody
      • V ATPase subunit d 1 antibody
      • V ATPase subunit D antibody
      • V-ATPase 40 kDa accessory protein antibody
      • V-ATPase AC39 subunit antibody
      • V-ATPase subunit d 1 antibody
      • V-type proton ATPase subunit d 1 antibody
      • VA0D1_HUMAN antibody
      • Vacuolar ATP synthase subunit d 1 antibody
      • Vacuolar proton pump subunit d 1 antibody
      • VATX antibody
      • VMA 6 antibody
      • VMA6 antibody
      • VPATPD antibody
      see all

    画像

    • Western blot - Anti-ATP6V0D1/P39 antibody [2G12] (ab56441)
      Western blot - Anti-ATP6V0D1/P39 antibody [2G12] (ab56441)

      ATP6V0D1/P39 antibody (ab56441) at 1ug/lane + HeLa cell lysate at 25ug/lane.

      This image was generated using the ascites version of the product.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP6V0D1/P39 antibody [2G12] (ab56441)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP6V0D1/P39 antibody [2G12] (ab56441)

      ATP6V0D1/P39 antibody (ab56441) used in immunohistochemistry at 5ug/ml on formalin fixed and paraffin embedded human stomach.

      This image was generated using the ascites version of the product.

    • Immunocytochemistry/ Immunofluorescence - Anti-ATP6V0D1/P39 antibody [2G12] (ab56441)
      Immunocytochemistry/ Immunofluorescence - Anti-ATP6V0D1/P39 antibody [2G12] (ab56441)

      ICC/IF image of ab56441 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab56441, 10µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

      This image was generated using the ascites version of the product.

    • Flow Cytometry - Anti-ATP6V0D1/P39 antibody [2G12] (ab56441)
      Flow Cytometry - Anti-ATP6V0D1/P39 antibody [2G12] (ab56441)

      Overlay histogram showing HeLa cells stained with ab56441 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab56441, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was a mix of mouse IgG1 [ICIGG1], (ab91353, 2μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4%PFA/permeabilized in 0.1% PBS-Tween used under the same conditions.

      This image was generated using the ascites version of the product.

    参考文献 (19)

    ab56441 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab56441 は 19 報の論文で使用されています。

    • Hooper KM  et al. V-ATPase is a universal regulator of LC3-associated phagocytosis and non-canonical autophagy. J Cell Biol 221:N/A (2022). PubMed: 35511089
    • Liu N  et al. ATP6V0d2 Suppresses Alveoli Macrophage Alternative Polarization and Allergic Asthma via Degradation of PU.1. Allergy Asthma Immunol Res 13:479-497 (2021). PubMed: 33733641
    • Li B  et al. Genome-wide CRISPR screen identifies host dependency factors for influenza A virus infection. Nat Commun 11:164 (2020). PubMed: 31919360
    • Larramona-Arcas R  et al. Sex-dependent calcium hyperactivity due to lysosomal-related dysfunction in astrocytes from APOE4 versus APOE3 gene targeted replacement mice. Mol Neurodegener 15:35 (2020). PubMed: 32517777
    • Huff TC  et al. Oscillatory cAMP signaling rapidly alters H3K4 methylation. Life Sci Alliance 3:N/A (2020). PubMed: 31882444
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-2 of 2 Abreviews or Q&A

    Western blot abreview for Anti-ATP6V0D1 antibody

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Pig Cell lysate - other (LLC-PK1)
    Gel Running Conditions
    Non-reduced Denaturing (4-15%)
    Loading amount
    10 µg
    Specification
    LLC-PK1
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 1.5% · Temperature: 21°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Christina Mcguire

    Verified customer

    投稿 Dec 22 2015

    Question

    Customer kindly contacted us as they were unable to see any signal when using the product in the following conditions:

    Sample:HeLa cells.  4% formaldehyde fixed (10 min)

    Blocking and Permeablization: 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h.

    Primary AB: incubated with the antibody (ab56441, 10µg/ml) overnight at +4°C.

    Secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L)

    These were the exact conditions listed on the datasheet. Further the customer tried with their own protocol, also with no result.

    Read More
    Answer

    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number xxxx.

    Read More

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