Anti-ATP5D 抗体 [4D11] (ab174438)
![Western blot - Anti-ATP5D antibody [4D11] (ab174438)](https://www.abcam.co.jp/ps/products/174/ab174438/Images/ab174438-199865-ab1744385.jpg "Western blot - Anti-ATP5D antibody [4D11] (ab174438)")
Key features and details
- Mouse monoclonal [4D11] to ATP5D
- Suitable for: WB
- Reacts with: Mouse, Rat, Cow, Human
- Isotype: IgG1
製品の概要
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製品名
Anti-ATP5D antibody [4D11]
ATP5D 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [4D11] to ATP5D -
由来種
Mouse -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse, Rat, Cow, Human -
免疫原
Tissue, cells or virus. This information is considered to be commercially sensitive.
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ポジティブ･コントロール
- Rat and mouse heart and liver homogenates, rat H4IIE lysate and mouse MEF cell lysate.
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特記事項
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: 99.9% HEPES buffered saline -
Concentration information loading... -
ポリ/モノ
モノクローナル -
クローン名
4D11 -
アイソタイプ
IgG1 -
軽鎖の種類
kappa -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab174438の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 17 kDa.
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| 特記事項 |
|---|
|
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 17 kDa. |
ターゲット情報
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機能
Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP turnover in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(1) domain and of the central stalk which is part of the complex rotary element. Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits. -
配列類似性
Belongs to the ATPase epsilon chain family. -
細胞内局在
Mitochondrion. Mitochondrion inner membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 338081 Cow
- Entrez Gene: 513 Human
- Entrez Gene: 66043 Mouse
- Entrez Gene: 245965 Rat
- Omim: 603150 Human
- SwissProt: P05630 Cow
- SwissProt: P30049 Human
- SwissProt: Q9D3D9 Mouse
see all -
別名
- ATP synthase subunit delta, mitochondrial antibody
- ATP synthase subunit delta, mitochondrial antibody
- ATP synthase, H+ transporting, mitochondrial F1 complex, delta subunit antibody
see all
画像
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Western blot - Anti-ATP5D antibody [4D11] (ab174438)All lanes : Anti-ATP5D antibody [4D11] (ab174438) at 1 µg/ml (5% milk PBST)
Lane 1 : Human Heart Homogenate at 10 µg
Lane 2 : Human Liver Homogenate at 10 µg
Lane 3 : Rat Liver Homogenate at 10 µg
Lane 4 : Mouse Liver Homogenate at 10 µg
Lane 5 : Bovine Heart Homogenate at 10 µg
Lane 6 : Human HepG2 cell lysate at 20 µg
Lane 7 : Rat H4IIE cell lysate at 20 µg
Lane 8 : Mouse MEF cell lysate at 20 µg
Secondary
All lanes : Goat anti Mouse IR700 in 5% milk/PBST at 1/5000 dilution
Predicted band size: 17 kDa
データシートおよび資料
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Datasheet download
参考文献 (0)
ab174438 は論文での使用が確認できていません。
